BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000058.1_g1510.1
(218 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G53490.1 | valine-tRNA ligase | Chr3:19832666-19834024 FORWAR... 216 5e-71
AT5G02720.2 | valine-tRNA ligase | Chr5:613782-615039 REVERSE LE... 192 8e-62
AT5G02720.1 | valine-tRNA ligase | Chr5:613782-614313 REVERSE LE... 117 3e-33
>AT3G53490.1 | valine-tRNA ligase | Chr3:19832666-19834024 FORWARD
LENGTH=225 | 201606
Length = 225
Score = 216 bits (550), Expect = 5e-71, Method: Compositional matrix adjust.
Identities = 120/217 (55%), Positives = 155/217 (71%), Gaps = 13/217 (5%)
Query: 12 FDVFFFCSLFLFFLPEGILSSGVVTLESIQIFKTHEWINNKPTVYFRCQGENKTILPDVK 71
F F C +F LP IL+ VTL+S+QIF TH+W + KPTV+F+C+GENKT+LPDVK
Sbjct: 12 FRGFLLCFIFFSSLPGTILTQ-EVTLDSVQIFTTHDWFSTKPTVFFQCKGENKTVLPDVK 70
Query: 72 KAHFLYTFKEEESWQPLTELD-KKCKRCGLYEEDTIETDDIFDAWEFCPSDFSAPNGNYT 130
+ + Y+F EESWQPLTEL KCKRCG+YE+D ++ D FD WE CPSDF+A G+Y
Sbjct: 71 RTNVSYSFNGEESWQPLTELQGTKCKRCGIYEDDPLKY-DTFDEWELCPSDFTA-EGSYK 128
Query: 131 RFKDKEFNATFLCPKCVGIGAGVFKGK---------GMNVLIVIVITALVSAISIIGIIY 181
RFK+KEFNATFLC C +GAG K GM+ IV++I L+ + +G++
Sbjct: 129 RFKEKEFNATFLCHGCSQVGAGSNKESGTEKEEQKGGMHPGIVVLIVVLLLGVVAVGLLV 188
Query: 182 GYRYWQKKKRQQDQARFLKLFEDDDDLEDELGLGSVI 218
GY+YW+KKKRQQ+QARFLKLFED DD+EDELGL + +
Sbjct: 189 GYKYWRKKKRQQEQARFLKLFEDGDDIEDELGLENTL 225
>AT5G02720.2 | valine-tRNA ligase | Chr5:613782-615039 REVERSE
LENGTH=225 | 201606
Length = 225
Score = 192 bits (489), Expect = 8e-62, Method: Compositional matrix adjust.
Identities = 95/222 (42%), Positives = 139/222 (62%), Gaps = 14/222 (6%)
Query: 9 PKPFDVFFFCSLFLFFLPEGILSSGVVTLESIQIFKTHEWINNKPTVYFRCQGENKTILP 68
P + FC + ++P G++ +TL SI+IF H+W K TVYF+C GENKT+LP
Sbjct: 6 PMIWRNLLFCYIIHMWIP-GLVRGQFITLNSIEIFTKHDWFKLKHTVYFQCNGENKTVLP 64
Query: 69 DVKKAHFLYTFKEEESWQPLTEL-DKKCKRCGLYEEDTIETDDIFDAWEFCPSDFSAPNG 127
DV K LYTF+ +ESWQP+T + +KCKRCG+YE+ ++ +D FD WE CP+DFSA
Sbjct: 65 DVTKTGVLYTFRGQESWQPMTGIGGEKCKRCGIYEQGSLVSDKEFDVWEVCPTDFSASQ- 123
Query: 128 NYTRFKDKEFNATFLCPKCVGIGAGVFKG-----------KGMNVLIVIVITALVSAISI 176
Y FK+KE NATF+C C + K G+ +I I+ L + + +
Sbjct: 124 VYMHFKEKEINATFVCHGCAKFHSVSSKAVAASSPQEEGYNGLTFMIAIIAGVLCTTLVV 183
Query: 177 IGIIYGYRYWQKKKRQQDQARFLKLFEDDDDLEDELGLGSVI 218
+G ++ +++ Q+ K+Q+DQARF++LFE+ D+ EDELGL VI
Sbjct: 184 VGGVFMFKHTQRMKKQRDQARFMQLFEESDEPEDELGLDPVI 225
>AT5G02720.1 | valine-tRNA ligase | Chr5:613782-614313 REVERSE
LENGTH=142 | 201606
Length = 142
Score = 117 bits (292), Expect = 3e-33, Method: Compositional matrix adjust.
Identities = 58/137 (42%), Positives = 87/137 (63%), Gaps = 12/137 (8%)
Query: 93 KKCKRCGLYEEDTIETDDIFDAWEFCPSDFSAPNGNYTRFKDKEFNATFLCPKCV----- 147
+KCKRCG+YE+ ++ +D FD WE CP+DFSA Y FK+KE NATF+C C
Sbjct: 7 EKCKRCGIYEQGSLVSDKEFDVWEVCPTDFSASQ-VYMHFKEKEINATFVCHGCAKFHSV 65
Query: 148 ---GIGAGVFKGKGMNVL---IVIVITALVSAISIIGIIYGYRYWQKKKRQQDQARFLKL 201
+ A + +G N L I I+ L + + ++G ++ +++ Q+ K+Q+DQARF++L
Sbjct: 66 SSKAVAASSPQEEGYNGLTFMIAIIAGVLCTTLVVVGGVFMFKHTQRMKKQRDQARFMQL 125
Query: 202 FEDDDDLEDELGLGSVI 218
FE+ D+ EDELGL VI
Sbjct: 126 FEESDEPEDELGLDPVI 142