BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Eca_sc000058.1_g2810.1
         (317 letters)

Database: Araport11_genes.201606.pep 
           48,359 sequences; 20,855,782 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G10250.5 | histidine-tRNA ligase | Chr3:3168371-3170418 REVER...   350   e-121
AT3G10250.4 | histidine-tRNA ligase | Chr3:3168371-3170418 REVER...   350   e-121
AT3G10250.2 | histidine-tRNA ligase | Chr3:3168371-3170418 REVER...   350   e-121
AT3G10250.1 | histidine-tRNA ligase | Chr3:3168371-3170418 REVER...   350   e-121
AT3G10250.3 | histidine-tRNA ligase | Chr3:3168781-3170418 REVER...   298   e-100

>AT3G10250.5 | histidine-tRNA ligase | Chr3:3168371-3170418 REVERSE
           LENGTH=324 | 201606
          Length = 324

 Score =  350 bits (899), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 187/325 (57%), Positives = 238/325 (73%), Gaps = 9/325 (2%)

Query: 1   MSSGEVRRVSRQDIQLVQNLIERCLQLYMNQKEVVETLLVEAKIEPGFTELVWQKLEEEN 60
           MSSG VRRVSRQDIQLVQNLIERCLQLYMNQKEVV+TLL +AKIEPGFTELVWQKLEEEN
Sbjct: 1   MSSGTVRRVSRQDIQLVQNLIERCLQLYMNQKEVVDTLLEQAKIEPGFTELVWQKLEEEN 60

Query: 61  REFFNAYNVRLVVKHQIMVFNELLGKQVELMRKTSPVGLGSLPMSNGSNSSSLHQRP-SY 119
           REFF AY +RL+VKHQIM FN+LL +QV  MR+  P G+ S+  +NGS+  S++Q+   Y
Sbjct: 61  REFFKAYYLRLMVKHQIMEFNKLLEQQVHHMRQMHPTGVASVQNTNGSHLQSMNQKQLCY 120

Query: 120 VSQQRMQPSSLENMHLTVGPSSRPS--NGRPAVHRSLQTANDTSTQTRKMDVSPNMLPAQ 177
            S+   Q    E+ H  +  S   +  NG   ++ ++ ++ + ST  R++D SPNML +Q
Sbjct: 121 PSEHTDQSLKSESAHHPMASSLSNAFLNGSSTLNTNVPSSINISTHARRVDASPNMLSSQ 180

Query: 178 NSHMSMMQGLNGTVIKTESGYSNSSPFAYGLEGNFLETRPTIGDAAVASFRSVEPNAQTL 237
            ++M MMQG+NG +IK+E+ ++N + F YG E N LE    +GD ++ +F S E N Q L
Sbjct: 181 TTNMPMMQGMNGGMIKSETAFTNPASFMYGGERNALEGHSAVGDTSIPNF-SNESNNQPL 239

Query: 238 DTSLLETDASSFGFLGQISRNFSLSDLTADFSQSSDILETYSRSPF----TDDFLDSPGR 293
              LLE +AS+FGFLGQI RNFSLSDLTADFSQSS+ILE+Y RSPF     ++FLDS  R
Sbjct: 240 SDPLLEAEASTFGFLGQIPRNFSLSDLTADFSQSSEILESYDRSPFLVPNAENFLDSRDR 299

Query: 294 -DCQGDNKRLDTISEGLSFEEFGSD 317
            + QGDNKRLDTISEG S++  GS+
Sbjct: 300 GEYQGDNKRLDTISEGFSYDNIGSE 324


>AT3G10250.4 | histidine-tRNA ligase | Chr3:3168371-3170418 REVERSE
           LENGTH=324 | 201606
          Length = 324

 Score =  350 bits (899), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 187/325 (57%), Positives = 238/325 (73%), Gaps = 9/325 (2%)

Query: 1   MSSGEVRRVSRQDIQLVQNLIERCLQLYMNQKEVVETLLVEAKIEPGFTELVWQKLEEEN 60
           MSSG VRRVSRQDIQLVQNLIERCLQLYMNQKEVV+TLL +AKIEPGFTELVWQKLEEEN
Sbjct: 1   MSSGTVRRVSRQDIQLVQNLIERCLQLYMNQKEVVDTLLEQAKIEPGFTELVWQKLEEEN 60

Query: 61  REFFNAYNVRLVVKHQIMVFNELLGKQVELMRKTSPVGLGSLPMSNGSNSSSLHQRP-SY 119
           REFF AY +RL+VKHQIM FN+LL +QV  MR+  P G+ S+  +NGS+  S++Q+   Y
Sbjct: 61  REFFKAYYLRLMVKHQIMEFNKLLEQQVHHMRQMHPTGVASVQNTNGSHLQSMNQKQLCY 120

Query: 120 VSQQRMQPSSLENMHLTVGPSSRPS--NGRPAVHRSLQTANDTSTQTRKMDVSPNMLPAQ 177
            S+   Q    E+ H  +  S   +  NG   ++ ++ ++ + ST  R++D SPNML +Q
Sbjct: 121 PSEHTDQSLKSESAHHPMASSLSNAFLNGSSTLNTNVPSSINISTHARRVDASPNMLSSQ 180

Query: 178 NSHMSMMQGLNGTVIKTESGYSNSSPFAYGLEGNFLETRPTIGDAAVASFRSVEPNAQTL 237
            ++M MMQG+NG +IK+E+ ++N + F YG E N LE    +GD ++ +F S E N Q L
Sbjct: 181 TTNMPMMQGMNGGMIKSETAFTNPASFMYGGERNALEGHSAVGDTSIPNF-SNESNNQPL 239

Query: 238 DTSLLETDASSFGFLGQISRNFSLSDLTADFSQSSDILETYSRSPF----TDDFLDSPGR 293
              LLE +AS+FGFLGQI RNFSLSDLTADFSQSS+ILE+Y RSPF     ++FLDS  R
Sbjct: 240 SDPLLEAEASTFGFLGQIPRNFSLSDLTADFSQSSEILESYDRSPFLVPNAENFLDSRDR 299

Query: 294 -DCQGDNKRLDTISEGLSFEEFGSD 317
            + QGDNKRLDTISEG S++  GS+
Sbjct: 300 GEYQGDNKRLDTISEGFSYDNIGSE 324


>AT3G10250.2 | histidine-tRNA ligase | Chr3:3168371-3170418 REVERSE
           LENGTH=324 | 201606
          Length = 324

 Score =  350 bits (899), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 187/325 (57%), Positives = 238/325 (73%), Gaps = 9/325 (2%)

Query: 1   MSSGEVRRVSRQDIQLVQNLIERCLQLYMNQKEVVETLLVEAKIEPGFTELVWQKLEEEN 60
           MSSG VRRVSRQDIQLVQNLIERCLQLYMNQKEVV+TLL +AKIEPGFTELVWQKLEEEN
Sbjct: 1   MSSGTVRRVSRQDIQLVQNLIERCLQLYMNQKEVVDTLLEQAKIEPGFTELVWQKLEEEN 60

Query: 61  REFFNAYNVRLVVKHQIMVFNELLGKQVELMRKTSPVGLGSLPMSNGSNSSSLHQRP-SY 119
           REFF AY +RL+VKHQIM FN+LL +QV  MR+  P G+ S+  +NGS+  S++Q+   Y
Sbjct: 61  REFFKAYYLRLMVKHQIMEFNKLLEQQVHHMRQMHPTGVASVQNTNGSHLQSMNQKQLCY 120

Query: 120 VSQQRMQPSSLENMHLTVGPSSRPS--NGRPAVHRSLQTANDTSTQTRKMDVSPNMLPAQ 177
            S+   Q    E+ H  +  S   +  NG   ++ ++ ++ + ST  R++D SPNML +Q
Sbjct: 121 PSEHTDQSLKSESAHHPMASSLSNAFLNGSSTLNTNVPSSINISTHARRVDASPNMLSSQ 180

Query: 178 NSHMSMMQGLNGTVIKTESGYSNSSPFAYGLEGNFLETRPTIGDAAVASFRSVEPNAQTL 237
            ++M MMQG+NG +IK+E+ ++N + F YG E N LE    +GD ++ +F S E N Q L
Sbjct: 181 TTNMPMMQGMNGGMIKSETAFTNPASFMYGGERNALEGHSAVGDTSIPNF-SNESNNQPL 239

Query: 238 DTSLLETDASSFGFLGQISRNFSLSDLTADFSQSSDILETYSRSPF----TDDFLDSPGR 293
              LLE +AS+FGFLGQI RNFSLSDLTADFSQSS+ILE+Y RSPF     ++FLDS  R
Sbjct: 240 SDPLLEAEASTFGFLGQIPRNFSLSDLTADFSQSSEILESYDRSPFLVPNAENFLDSRDR 299

Query: 294 -DCQGDNKRLDTISEGLSFEEFGSD 317
            + QGDNKRLDTISEG S++  GS+
Sbjct: 300 GEYQGDNKRLDTISEGFSYDNIGSE 324


>AT3G10250.1 | histidine-tRNA ligase | Chr3:3168371-3170418 REVERSE
           LENGTH=324 | 201606
          Length = 324

 Score =  350 bits (899), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 187/325 (57%), Positives = 238/325 (73%), Gaps = 9/325 (2%)

Query: 1   MSSGEVRRVSRQDIQLVQNLIERCLQLYMNQKEVVETLLVEAKIEPGFTELVWQKLEEEN 60
           MSSG VRRVSRQDIQLVQNLIERCLQLYMNQKEVV+TLL +AKIEPGFTELVWQKLEEEN
Sbjct: 1   MSSGTVRRVSRQDIQLVQNLIERCLQLYMNQKEVVDTLLEQAKIEPGFTELVWQKLEEEN 60

Query: 61  REFFNAYNVRLVVKHQIMVFNELLGKQVELMRKTSPVGLGSLPMSNGSNSSSLHQRP-SY 119
           REFF AY +RL+VKHQIM FN+LL +QV  MR+  P G+ S+  +NGS+  S++Q+   Y
Sbjct: 61  REFFKAYYLRLMVKHQIMEFNKLLEQQVHHMRQMHPTGVASVQNTNGSHLQSMNQKQLCY 120

Query: 120 VSQQRMQPSSLENMHLTVGPSSRPS--NGRPAVHRSLQTANDTSTQTRKMDVSPNMLPAQ 177
            S+   Q    E+ H  +  S   +  NG   ++ ++ ++ + ST  R++D SPNML +Q
Sbjct: 121 PSEHTDQSLKSESAHHPMASSLSNAFLNGSSTLNTNVPSSINISTHARRVDASPNMLSSQ 180

Query: 178 NSHMSMMQGLNGTVIKTESGYSNSSPFAYGLEGNFLETRPTIGDAAVASFRSVEPNAQTL 237
            ++M MMQG+NG +IK+E+ ++N + F YG E N LE    +GD ++ +F S E N Q L
Sbjct: 181 TTNMPMMQGMNGGMIKSETAFTNPASFMYGGERNALEGHSAVGDTSIPNF-SNESNNQPL 239

Query: 238 DTSLLETDASSFGFLGQISRNFSLSDLTADFSQSSDILETYSRSPF----TDDFLDSPGR 293
              LLE +AS+FGFLGQI RNFSLSDLTADFSQSS+ILE+Y RSPF     ++FLDS  R
Sbjct: 240 SDPLLEAEASTFGFLGQIPRNFSLSDLTADFSQSSEILESYDRSPFLVPNAENFLDSRDR 299

Query: 294 -DCQGDNKRLDTISEGLSFEEFGSD 317
            + QGDNKRLDTISEG S++  GS+
Sbjct: 300 GEYQGDNKRLDTISEGFSYDNIGSE 324


>AT3G10250.3 | histidine-tRNA ligase | Chr3:3168781-3170418 REVERSE
           LENGTH=297 | 201606
          Length = 297

 Score =  298 bits (763), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 158/277 (57%), Positives = 202/277 (72%), Gaps = 4/277 (1%)

Query: 1   MSSGEVRRVSRQDIQLVQNLIERCLQLYMNQKEVVETLLVEAKIEPGFTELVWQKLEEEN 60
           MSSG VRRVSRQDIQLVQNLIERCLQLYMNQKEVV+TLL +AKIEPGFTELVWQKLEEEN
Sbjct: 1   MSSGTVRRVSRQDIQLVQNLIERCLQLYMNQKEVVDTLLEQAKIEPGFTELVWQKLEEEN 60

Query: 61  REFFNAYNVRLVVKHQIMVFNELLGKQVELMRKTSPVGLGSLPMSNGSNSSSLHQRP-SY 119
           REFF AY +RL+VKHQIM FN+LL +QV  MR+  P G+ S+  +NGS+  S++Q+   Y
Sbjct: 61  REFFKAYYLRLMVKHQIMEFNKLLEQQVHHMRQMHPTGVASVQNTNGSHLQSMNQKQLCY 120

Query: 120 VSQQRMQPSSLENMHLTVGPSSRPS--NGRPAVHRSLQTANDTSTQTRKMDVSPNMLPAQ 177
            S+   Q    E+ H  +  S   +  NG   ++ ++ ++ + ST  R++D SPNML +Q
Sbjct: 121 PSEHTDQSLKSESAHHPMASSLSNAFLNGSSTLNTNVPSSINISTHARRVDASPNMLSSQ 180

Query: 178 NSHMSMMQGLNGTVIKTESGYSNSSPFAYGLEGNFLETRPTIGDAAVASFRSVEPNAQTL 237
            ++M MMQG+NG +IK+E+ ++N + F YG E N LE    +GD ++ +F S E N Q L
Sbjct: 181 TTNMPMMQGMNGGMIKSETAFTNPASFMYGGERNALEGHSAVGDTSIPNF-SNESNNQPL 239

Query: 238 DTSLLETDASSFGFLGQISRNFSLSDLTADFSQSSDI 274
              LLE +AS+FGFLGQI RNFSLSDLTADFSQSS +
Sbjct: 240 SDPLLEAEASTFGFLGQIPRNFSLSDLTADFSQSSGL 276


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