BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000058.1_g4050.1
(255 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G16010.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family prote... 288 3e-98
AT2G38050.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family prote... 73 6e-15
AT3G55360.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family prote... 67 8e-13
AT1G72590.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family prote... 65 5e-12
AT2G16530.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase family prote... 65 6e-12
>AT5G16010.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein
| Chr5:5227982-5229012 FORWARD LENGTH=268 | 201606
Length = 268
Score = 288 bits (737), Expect = 3e-98, Method: Compositional matrix adjust.
Identities = 141/256 (55%), Positives = 183/256 (71%), Gaps = 8/256 (3%)
Query: 1 MSAISFISLANAGISEIKGKHLQYSKFWNANNNNSSQKSSSSKINKEEIKIPSRIGMLIL 60
MS + +LAN G SEI+G HL+YSKF ++++ QK I+ SR GML+L
Sbjct: 20 MSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERFGSIS-------SRNGMLLL 72
Query: 61 YTPAFLAGAISLLIFPNHDLRFLLVTSALTLHFFKRDFEVLFVHSYSGKMILDTTLMISL 120
YTPAFLA A S + P+ DLRFLL+ SAL LHFFKR FEVLF+H YSG M +D+ L IS
Sbjct: 73 YTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSGGMAIDSALTISS 132
Query: 121 SYFLSTVSMIYNQHLIQGIPDPPIDLKSVGIAVFLVGMIGNFYHHYILSTLRNKN-DKGY 179
SYF ST M+Y+Q+L G+ +P D+K G+ +F+VG++GN YHH +L+ LR ++ K Y
Sbjct: 133 SYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKLRKEDGKKEY 192
Query: 180 KIPKGGLFGFVVCPHYLFEILIFLGISLTSQTVYPFCFTIGTACYLTGRSYATKEWYLSK 239
KIPKGGLF ++CPHYLFEIL+F L SQT+Y F F +GT YL GRSYAT+ WYLSK
Sbjct: 193 KIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSYATRTWYLSK 252
Query: 240 FPDFPKHVKSIVPFLF 255
F DFPKH+K+++PF+F
Sbjct: 253 FDDFPKHIKALIPFVF 268
>AT2G38050.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein
| Chr2:15921303-15922176 REVERSE LENGTH=262 | 201606
Length = 262
Score = 72.8 bits (177), Expect = 6e-15, Method: Compositional matrix adjust.
Identities = 58/215 (26%), Positives = 99/215 (46%), Gaps = 17/215 (7%)
Query: 55 IGMLILYTPAFLAGAISLLIFP--NHDL--RFLLVTSALTLHFFKRD-------FEVLFV 103
I ++ +P ++LL+FP H L + LL+ S +H+F R F F
Sbjct: 51 IAWFVMESPTLW---LTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFP 107
Query: 104 HSYSGKMILDTTLMISLSYFLSTVSMIYNQHLIQGIPDPPIDLKS--VGIAVFLVGMIGN 161
+G I L + + + + H D +G+ VF+ GM N
Sbjct: 108 AGKNGFPITIAALAFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYIN 167
Query: 162 FYHHYILSTLRNKNDKGYKIPKGGLFGFVVCPHYLFEILIFLGISLTSQTVYPFCFTIGT 221
L L+ +N GY IP+GG F V CP+Y E + +LG ++ + + F + T
Sbjct: 168 ITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYT 227
Query: 222 ACYLTGRSYATKEWYLSKF-PDFPKHVKSIVPFLF 255
L R+ A+ +WY++KF ++PK K+++PF++
Sbjct: 228 CSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262
>AT3G55360.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein
| Chr3:20521186-20522856 REVERSE LENGTH=310 | 201606
Length = 310
Score = 67.4 bits (163), Expect = 8e-13, Method: Compositional matrix adjust.
Identities = 53/174 (30%), Positives = 84/174 (48%), Gaps = 13/174 (7%)
Query: 91 LHFFKRDFEVLFVHSYSGKMILDTTLMISLSYFLSTVSMI--YNQHLIQGIPDPPIDLK- 147
H+FKR E FVH +S + + +Y+ S + I Y H + P DL+
Sbjct: 140 FHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLY---TPVSDLQM 196
Query: 148 SVGIAVFLVGMIGNFYHHYILSTLRNKNDKG-YKIPKGGLFGFVVCPHYLFEILIFLGIS 206
+G LV + NFY H +L LR+ + G Y+IP+G LF V C +Y EI +LG +
Sbjct: 197 KIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFN 256
Query: 207 LTSQTVYPFCFTIGTACYLT----GRSYATKEWYLSK--FPDFPKHVKSIVPFL 254
+ +QT+ + F A +T G+ ++ + K P +P+ + PFL
Sbjct: 257 IATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL 310
>AT1G72590.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein
| Chr1:27335601-27336989 FORWARD LENGTH=320 | 201606
Length = 320
Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust.
Identities = 41/118 (34%), Positives = 57/118 (48%), Gaps = 10/118 (8%)
Query: 145 DLKSVGIAVFLVGMIGNFYHHYILSTLRN--KNDKGYKIPKGGLFGFVVCPHYLFEILIF 202
L+ +G A+FL G I H IL +LR K Y IP G F V CPH+L EI+++
Sbjct: 206 SLQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLY 265
Query: 203 LGISLTS-----QTVYPFCFTIGTACYLTGRSYATKEWYLSKFPDFPKHVKSIVPFLF 255
LG+ ++S F F Y G T WYL KF ++P +I P ++
Sbjct: 266 LGLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320
>AT2G16530.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein
| Chr2:7163276-7165064 REVERSE LENGTH=342 | 201606
Length = 342
Score = 65.1 bits (157), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 41/118 (34%), Positives = 60/118 (50%), Gaps = 10/118 (8%)
Query: 145 DLKSVGIAVFLVGMIGNFYHHYILSTLRNK--NDKGYKIPKGGLFGFVVCPHYLFEILIF 202
L+ +G A+FL G I H IL +LR K Y IP G FG V PH+L EI+++
Sbjct: 228 SLQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLY 287
Query: 203 LGISLTSQ----TVY-PFCFTIGTACYLTGRSYATKEWYLSKFPDFPKHVKSIVPFLF 255
G+ + S T++ F F Y G T WYL KF ++P + +I P+++
Sbjct: 288 AGLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342