BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Eca_sc000058.1_g4240.1
         (167 letters)

Database: Araport11_genes.201606.pep 
           48,359 sequences; 20,855,782 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G10020.1 | hydroxysteroid dehydrogenase 5 | Chr4:6268363-6270...   213   9e-69
AT5G50700.1 | hydroxysteroid dehydrogenase 1 | Chr5:20623259-206...    87   1e-20
AT5G50600.1 | hydroxysteroid dehydrogenase 1 | Chr5:20589917-205...    87   1e-20
AT5G50770.1 | hydroxysteroid dehydrogenase 6 | Chr5:20646790-206...    82   8e-19
AT5G50690.1 | hydroxysteroid dehydrogenase 7 | Chr5:20621330-206...    65   6e-13

>AT4G10020.1 | hydroxysteroid dehydrogenase 5 | Chr4:6268363-6270179
           FORWARD LENGTH=389 | 201606
          Length = 389

 Score =  213 bits (543), Expect = 9e-69,   Method: Compositional matrix adjust.
 Identities = 102/163 (62%), Positives = 132/163 (80%), Gaps = 4/163 (2%)

Query: 1   MSLYAAAKAAVINFYETLRFEVGDEVGITVATHGWIGSELAGGRLMLEEGAAEMQWREER 60
           MSLY+AAKAA++NFYETLRFE+  +VGIT+ATHGWIGSE++GG+ MLEEGA EMQW+EER
Sbjct: 196 MSLYSAAKAALVNFYETLRFELNGDVGITIATHGWIGSEMSGGKFMLEEGA-EMQWKEER 254

Query: 61  EAPPNNGHVEEFAKLIVSGACRGDPYVKLPNWYNIFTLYRVFAPNVLTWTFRILFASNGS 120
           E P N G +EEFAK+IV+GACRGD YVK PNWY++F LYRVF PNVL WTF++L ++ G+
Sbjct: 255 EVPANGGPLEEFAKMIVAGACRGDAYVKFPNWYDVFLLYRVFTPNVLRWTFKLLLSTEGT 314

Query: 121 SRRAISYMGTGRPLLESSSPRKL-IEAPPPRRLVAAAPLTFSS 162
            R ++  +G+G P+ ESSS  KL +E  PPR  V A+P  +++
Sbjct: 315 RRSSLVGVGSGMPVDESSSQMKLMLEGGPPR--VPASPPRYTA 355


>AT5G50700.1 | hydroxysteroid dehydrogenase 1 |
           Chr5:20623259-20624995 REVERSE LENGTH=349 | 201606
          Length = 349

 Score = 87.0 bits (214), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 44/119 (36%), Positives = 68/119 (57%), Gaps = 2/119 (1%)

Query: 1   MSLYAAAKAAVINFYETLRFEVGDEVGITVATHGWIGSELAGGRLMLEEG--AAEMQWRE 58
           MS Y A+KAA+++F+ET+R E+G +V IT+ T G+I SEL  G+    EG        R+
Sbjct: 194 MSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFSGEGELIVNQDMRD 253

Query: 59  EREAPPNNGHVEEFAKLIVSGACRGDPYVKLPNWYNIFTLYRVFAPNVLTWTFRILFAS 117
            +  P         AK IV+G CR   YV  P+W+ +  L++V  P ++ W  R+L+ +
Sbjct: 254 VQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPELIEWGCRLLYMT 312


>AT5G50600.1 | hydroxysteroid dehydrogenase 1 |
           Chr5:20589917-20591653 REVERSE LENGTH=349 | 201606
          Length = 349

 Score = 87.0 bits (214), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 44/119 (36%), Positives = 68/119 (57%), Gaps = 2/119 (1%)

Query: 1   MSLYAAAKAAVINFYETLRFEVGDEVGITVATHGWIGSELAGGRLMLEEG--AAEMQWRE 58
           MS Y A+KAA+++F+ET+R E+G +V IT+ T G+I SEL  G+    EG        R+
Sbjct: 194 MSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFSGEGELIVNQDMRD 253

Query: 59  EREAPPNNGHVEEFAKLIVSGACRGDPYVKLPNWYNIFTLYRVFAPNVLTWTFRILFAS 117
            +  P         AK IV+G CR   YV  P+W+ +  L++V  P ++ W  R+L+ +
Sbjct: 254 VQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPELIEWGCRLLYMT 312


>AT5G50770.1 | hydroxysteroid dehydrogenase 6 |
           Chr5:20646790-20648696 REVERSE LENGTH=342 | 201606
          Length = 342

 Score = 82.0 bits (201), Expect = 8e-19,   Method: Compositional matrix adjust.
 Identities = 41/114 (35%), Positives = 62/114 (54%), Gaps = 2/114 (1%)

Query: 1   MSLYAAAKAAVINFYETLRFEVGDEVGITVATHGWIGSELAGGRLMLEEG--AAEMQWRE 58
           +S Y A+KAAVI FYETLR E G ++G+T+   G + SE++ G+ M ++G    + + R+
Sbjct: 194 LSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMTKDGKLVVDKELRD 253

Query: 59  EREAPPNNGHVEEFAKLIVSGACRGDPYVKLPNWYNIFTLYRVFAPNVLTWTFR 112
            + +       E  AK I+   CRGD Y+  P+W     L +VF      W  R
Sbjct: 254 VQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSEATEWVAR 307


>AT5G50690.1 | hydroxysteroid dehydrogenase 7 |
           Chr5:20621330-20622638 FORWARD LENGTH=299 | 201606
          Length = 299

 Score = 65.5 bits (158), Expect = 6e-13,   Method: Compositional matrix adjust.
 Identities = 40/107 (37%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query: 1   MSLYAAAKAAVINFYETLRFEVGDEVGITVATHGWIGSELAGGRLMLEEGAAEMQWREER 60
           MS+YAA+KAA+INFYETLR E+  EVG+T+   G I +      L+ E+         E 
Sbjct: 194 MSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLAEKQDWSQVVTIES 253

Query: 61  EAPPNNGHVEEFAKLIVSGACRGDPYVKLPNWYNIFTLYRVFAPNVL 107
            A        E AK +V+G CRG  +V  P+W  +        P +L
Sbjct: 254 AA--------ECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPELL 292


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