BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000081.1_g2550.1
(302 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G13710.2 | sterol methyltransferase 1 | Chr5:4424048-4426866 ... 507 0.0
AT5G13710.1 | sterol methyltransferase 1 | Chr5:4424048-4426866 ... 507 0.0
AT1G20330.1 | sterol methyltransferase 2 | Chr1:7038968-7040053 ... 244 1e-78
AT1G76090.1 | sterol methyltransferase 3 | Chr1:28550592-2855167... 238 1e-76
AT1G73600.1 | S-adenosyl-L-methionine-dependent methyltransferas... 74 2e-14
>AT5G13710.2 | sterol methyltransferase 1 | Chr5:4424048-4426866
REVERSE LENGTH=336 | 201606
Length = 336
Score = 507 bits (1306), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 245/335 (73%), Positives = 271/335 (80%), Gaps = 42/335 (12%)
Query: 7 LDLASGVGGKIEKKEVLSAVDKYEKYHVSYGGEESERKANYTDMVNKYYDLVTSFYEYGW 66
+DLAS +GGKI+K +VL+AV+KYE+YHV +GG E ERKANYTDMVNKYYDL TSFYEYGW
Sbjct: 1 MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60
Query: 67 GESFHFAHRWKGESLRESIKRHEHFLALNLELKPGQKVLDVGCGIGGPLREIARFSSASV 126
GESFHFA RWKGESLRESIKRHEHFLAL L ++PGQKVLDVGCGIGGPLREIARFS++ V
Sbjct: 61 GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120
Query: 127 TGLNNNEYQITRGKELNRIAGVDKTCDFVKADFMKMPFSDNTFDAVYAVEATCHAPDALG 186
TGLNNNEYQITRGKELNR+AGVDKTC+FVKADFMKMPF +N+FDAVYA+EATCHAPDA G
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180
Query: 187 CYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQDHQKIKAAIELGDGLPDIRLTTQCLEALK 246
CYKEIYRVLKPGQCFAAYEWCMTD+FDP+N +HQKIK IE+GDGLPDIRLTT+CLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240
Query: 247 QAGFE------------------------------------------VKGLEYVGLAPAG 264
QAGFE VK LEY+ LAP G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300
Query: 265 SLRVQMFLEEAAEGLVGGGKKEIFTPMYFFLARKP 299
S RV FLE+AAEGLV GG++EIFTPMYFFLARKP
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335
>AT5G13710.1 | sterol methyltransferase 1 | Chr5:4424048-4426866
REVERSE LENGTH=336 | 201606
Length = 336
Score = 507 bits (1306), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 245/335 (73%), Positives = 271/335 (80%), Gaps = 42/335 (12%)
Query: 7 LDLASGVGGKIEKKEVLSAVDKYEKYHVSYGGEESERKANYTDMVNKYYDLVTSFYEYGW 66
+DLAS +GGKI+K +VL+AV+KYE+YHV +GG E ERKANYTDMVNKYYDL TSFYEYGW
Sbjct: 1 MDLASNLGGKIDKSDVLTAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGW 60
Query: 67 GESFHFAHRWKGESLRESIKRHEHFLALNLELKPGQKVLDVGCGIGGPLREIARFSSASV 126
GESFHFA RWKGESLRESIKRHEHFLAL L ++PGQKVLDVGCGIGGPLREIARFS++ V
Sbjct: 61 GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVV 120
Query: 127 TGLNNNEYQITRGKELNRIAGVDKTCDFVKADFMKMPFSDNTFDAVYAVEATCHAPDALG 186
TGLNNNEYQITRGKELNR+AGVDKTC+FVKADFMKMPF +N+FDAVYA+EATCHAPDA G
Sbjct: 121 TGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYG 180
Query: 187 CYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQDHQKIKAAIELGDGLPDIRLTTQCLEALK 246
CYKEIYRVLKPGQCFAAYEWCMTD+FDP+N +HQKIK IE+GDGLPDIRLTT+CLEALK
Sbjct: 181 CYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALK 240
Query: 247 QAGFE------------------------------------------VKGLEYVGLAPAG 264
QAGFE VK LEY+ LAP G
Sbjct: 241 QAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQG 300
Query: 265 SLRVQMFLEEAAEGLVGGGKKEIFTPMYFFLARKP 299
S RV FLE+AAEGLV GG++EIFTPMYFFLARKP
Sbjct: 301 SQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 335
>AT1G20330.1 | sterol methyltransferase 2 | Chr1:7038968-7040053
REVERSE LENGTH=361 | 201606
Length = 361
Score = 244 bits (622), Expect = 1e-78, Method: Compositional matrix adjust.
Identities = 128/327 (39%), Positives = 184/327 (56%), Gaps = 39/327 (11%)
Query: 6 ALDLASGVGGKIEKKEVLSAVDKYEKYHVSYG-GEESERKANYTDMVNKYYDLVTSFYEY 64
A+DL+ GG I ++V D Y++Y + +E E D V+ +Y+LVT YE+
Sbjct: 34 AVDLS---GGSISAEKVQ---DNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEW 87
Query: 65 GWGESFHFAHRWKGESLRESIKRHEHFLALNLELKPGQKVLDVGCGIGGPLREIARFSSA 124
GWG+SFHF+ G+S +++ + HE +++KPGQK+LDVGCG+GGP+R IA S A
Sbjct: 88 GWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRA 147
Query: 125 SVTGLNNNEYQITRGKELNRIAGVDKTCDFVKADFMKMPFSDNTFDAVYAVEATCHAPDA 184
+V G+ NEYQ+ R + N+ AG+D C+ V +F++MPF DN+FD Y++EATCHAP
Sbjct: 148 NVVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKL 207
Query: 185 LGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQDHQKIKAAIELGDGLPDIRLTTQCLEA 244
Y EIYRVLKPG + +YEW T+ F + +H ++ IE GD LP +R E
Sbjct: 208 EEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAET 267
Query: 245 LKQAGFE--------------------------------VKGLEYVGLAPAGSLRVQMFL 272
K+ GFE V+ L VG+AP G++ V L
Sbjct: 268 AKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEML 327
Query: 273 EEAAEGLVGGGKKEIFTPMYFFLARKP 299
+ A+ L GG+ IF+PM+ L RKP
Sbjct: 328 FKTADYLTRGGETGIFSPMHMILCRKP 354
>AT1G76090.1 | sterol methyltransferase 3 | Chr1:28550592-28551671
REVERSE LENGTH=359 | 201606
Length = 359
Score = 238 bits (608), Expect = 1e-76, Method: Compositional matrix adjust.
Identities = 123/319 (38%), Positives = 177/319 (55%), Gaps = 36/319 (11%)
Query: 14 GGKIEKKEVLSAVDKYEKYHVSY-GGEESERKANYTDMVNKYYDLVTSFYEYGWGESFHF 72
GG I ++V D Y +Y + +E E D V+ +Y+LVT YE+GWG+SFHF
Sbjct: 39 GGSISAEKVK---DNYNQYWSFFRKPKEIESAEKVPDFVDTFYNLVTDIYEWGWGQSFHF 95
Query: 73 AHRWKGESLRESIKRHEHFLALNLELKPGQKVLDVGCGIGGPLREIARFSSASVTGLNNN 132
+ G+S +++ + HE +++KPGQK+LD GCG+GGP+R IA S A VTG+ N
Sbjct: 96 SPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAIAAHSKAQVTGITIN 155
Query: 133 EYQITRGKELNRIAGVDKTCDFVKADFMKMPFSDNTFDAVYAVEATCHAPDALGCYKEIY 192
EYQ+ R K N+ AG+D C+ V +F+KMPF +NTFD Y++EATCHAP Y EI+
Sbjct: 156 EYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYSIEATCHAPKLEEVYSEIF 215
Query: 193 RVLKPGQCFAAYEWCMTDSFDPNNQDHQKIKAAIELGDGLPDIRLTTQCLEALKQAGFEV 252
RV+KPG F +YEW T+ + ++++H+ + IE GD LP +R K+ GFEV
Sbjct: 216 RVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEV 275
Query: 253 KG--------------------------------LEYVGLAPAGSLRVQMFLEEAAEGLV 280
L +G+AP G++ V L + A+ L
Sbjct: 276 VKEKDLAKPPSKPWWNRLKMGRIAYWRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLT 335
Query: 281 GGGKKEIFTPMYFFLARKP 299
GG+ IF+PM+ L RKP
Sbjct: 336 RGGETGIFSPMHMILCRKP 354
>AT1G73600.1 | S-adenosyl-L-methionine-dependent methyltransferases
superfamily protein | Chr1:27670825-27673400 FORWARD
LENGTH=490 | 201606
Length = 490
Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust.
Identities = 41/114 (35%), Positives = 60/114 (52%), Gaps = 2/114 (1%)
Query: 96 LELKPGQKVLDVGCGIGGPLREIARFSSASVTGLNNNEYQITRGKELNRIAGVDKTCDFV 155
L+LKPGQKVLDVGCGIGG +A V G++ + I+ L G+ + +F
Sbjct: 277 LDLKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFA--LEHAIGLKCSVEFE 334
Query: 156 KADFMKMPFSDNTFDAVYAVEATCHAPDALGCYKEIYRVLKPGQCFAAYEWCMT 209
AD K + DNTFD +Y+ + H D ++ Y+ LKPG ++C +
Sbjct: 335 VADCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVLITDYCRS 388