BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000081.1_g2760.1
(333 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G28780.1 | PIF1 helicase | Chr5:10812907-10814173 REVERSE LEN... 171 3e-50
AT3G51700.1 | PIF1 helicase | Chr3:19179443-19181145 REVERSE LEN... 159 8e-46
AT3G51690.1 | PIF1 helicase | Chr3:19176731-19178107 REVERSE LEN... 153 1e-43
>AT5G28780.1 | PIF1 helicase | Chr5:10812907-10814173 REVERSE
LENGTH=337 | 201606
Length = 337
Score = 171 bits (432), Expect = 3e-50, Method: Compositional matrix adjust.
Identities = 103/248 (41%), Positives = 147/248 (59%), Gaps = 12/248 (4%)
Query: 32 LIHRCKNLSELLCSTYPGLNILGTSSP--KYLTERAILSPRNDDVDFINSEALKIFPEET 89
L H L ++ Y ++ S P +YLTER IL+P N+ VD IN+ L ++
Sbjct: 87 LKHNGNRLQQVTKGAYVQFSV---SQPNFQYLTERGILTPHNEYVDEINAYMLSQVGGDS 143
Query: 90 IVYLAADKLVEEGEDNRLGGGN---YPNEYLNSLDPPGLPPFRLQLKVGCPVMLLRNLAP 146
YL++ + G+ + +G Y +YLNSL+ P LP ++ LK G P+M +RN
Sbjct: 144 KEYLSSYSI---GKADTIGADYEALYHVKYLNSLEFPSLPKHKISLKKGVPIMQMRNFNQ 200
Query: 147 KDGLCNGTRLIVVRCAPRIIEAQIITGDKSRDLVFLPRISLTTSSVEVPVRMTRRQFPIR 206
K+GLCNGTRLIV ++IEAQI+TG + +V +PR L+ E P + R+QFP+R
Sbjct: 201 KEGLCNGTRLIVTNLGEQVIEAQIVTGTHAGKMVSIPRFILSPPQSEHPFTLRRQQFPMR 260
Query: 207 LAYAMTINKSQGQSVKFGGVDLRTPVFSHGQLYVALSRCTASNRLHVLLPETEQNS-TTN 265
+ YAMTI K+QGQS+K + L PVFSH QLYVALSR T+ L +L + ++N N
Sbjct: 261 VCYAMTIIKNQGQSLKSDVLYLPNPVFSHVQLYVALSRVTSPIGLTILHGDDQKNDEVKN 320
Query: 266 IVYPESWD 273
IVY E ++
Sbjct: 321 IVYKEFYN 328
>AT3G51700.1 | PIF1 helicase | Chr3:19179443-19181145 REVERSE
LENGTH=344 | 201606
Length = 344
Score = 159 bits (402), Expect = 8e-46, Method: Compositional matrix adjust.
Identities = 100/266 (37%), Positives = 147/266 (55%), Gaps = 13/266 (4%)
Query: 21 GSNPCEIVQLPLIHRCKNLSELLCSTYPGLNILGTSSPKYLTERAILSPRNDDVDFINSE 80
G +I + LI CK+ + + G + + +P + ERAIL ND D IN
Sbjct: 78 GETKIDIHEDLLITECKDPIKTIVDEVYGESFTESYNPDFYQERAILCHTNDVADEINDY 137
Query: 81 AL-KIFPEETIVYLAADKLV---EEGEDNRLGGGNYPNEYLNSLDPPGLPPFRLQLKVGC 136
L ++ EET Y AD + D L YP E+LNS+ PG P F+L+LKVG
Sbjct: 138 MLSQLQGEETKCY-GADTIYPTHASPNDKML----YPLEFLNSIKIPGFPDFKLRLKVGA 192
Query: 137 PVMLLRNLAPKDGLCNGTRLIVVRCAPRIIEAQIITGDKSRDLVFLPRISLTTSSVEVPV 196
PVMLLR+LAP L GTRL + R ++EA IITG+ + V +PRI + P+
Sbjct: 193 PVMLLRDLAPYGWLRKGTRLQITRVETFVLEAMIITGNNHGEKVLIPRIPSDLREAKFPI 252
Query: 197 RMTRRQFPIRLAYAMTINKSQGQSVKFGGVDLRTPVFSHGQLYVALSRCTASNRLHVLLP 256
+M RRQFP++LA+AMTI++SQ Q++ G+ L + HGQ YVA+S+ + L VL+
Sbjct: 253 KMRRRQFPVKLAFAMTIDESQRQTLSKVGIYLPRQLLFHGQRYVAISKVKSRAGLKVLIT 312
Query: 257 ET----EQNSTTNIVYPESWDQQVQD 278
+ +Q T N+V+ E + + + +
Sbjct: 313 DKDGKPDQEETKNVVFKELFTEHLSE 338
>AT3G51690.1 | PIF1 helicase | Chr3:19176731-19178107 REVERSE
LENGTH=331 | 201606
Length = 331
Score = 153 bits (387), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 93/254 (36%), Positives = 140/254 (55%), Gaps = 8/254 (3%)
Query: 21 GSNPCEIVQLPLIHRCKNLSELLCSTYPGLNILGTSSPKYLTERAILSPRNDDVDFINSE 80
G +I + LI K+ + L G + +P + + AIL R+DDVD IN
Sbjct: 77 GETEIDISKDLLITESKDPIKTLLKEVYGEYFAKSYNPDFCHDSAILCHRDDDVDQINDY 136
Query: 81 ALKIFPEETIVYLAADKLVEEGEDNRLGGGNYPNEYLNSLDPPGLPPFRLQLKVGCPVML 140
L + P E L+ D + D+ P E LNS+ PGLP F+L+LKVG PVML
Sbjct: 137 MLSLLPGEEKECLSTDSISPSPNDDMF----VPLEVLNSIKVPGLPDFKLRLKVGAPVML 192
Query: 141 LRNLAPKDGLCNGTRLIVVRCAPRIIEAQIITGDKSRDLVFLPRISLTTSSVEVPVRMTR 200
LR+L P G GTRL + R ++EA II G+K +++PRI+ + P++M R
Sbjct: 193 LRDLDPSRGFFTGTRLQITRLCGFLLEAMIIAGNKHGKKIWIPRIASYPTETNFPLQMRR 252
Query: 201 RQFPIRLAYAMTINKSQGQSVKFGGVDLRTPVFSHG-QLYVALSRCTASNRLHVLLPETE 259
Q+P++LA+AMTI++SQ ++ G+ L VFSHG Q++VA+S+ + L VL+ + +
Sbjct: 253 TQYPLKLAFAMTIDESQVHTLSKVGLYLPRQVFSHGRQMFVAISKVKSRAGLKVLITDKD 312
Query: 260 ---QNSTTNIVYPE 270
Q N+V+ E
Sbjct: 313 GNPQEEAKNVVFKE 326