BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000102.1_g0860.1
(439 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G01090.1 | pyruvate dehydrogenase E1 alpha | Chr1:47705-49166... 701 0.0
AT1G59900.1 | pyruvate dehydrogenase complex E1 alpha subunit | ... 247 6e-78
AT1G59900.2 | pyruvate dehydrogenase complex E1 alpha subunit | ... 244 1e-76
AT1G24180.1 | Thiamin diphosphate-binding fold (THDP-binding) su... 237 8e-74
AT5G09300.2 | Thiamin diphosphate-binding fold (THDP-binding) su... 123 7e-31
>AT1G01090.1 | pyruvate dehydrogenase E1 alpha | Chr1:47705-49166
REVERSE LENGTH=428 | 201606
Length = 428
Score = 701 bits (1809), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 342/438 (78%), Positives = 379/438 (86%), Gaps = 12/438 (2%)
Query: 3 SSAFSPFKIIQPLSFSSTTRSPENSLFDPLK-STPTSFLASTQKFRSISSSKASPPSNNR 61
++AF+P K+ + S EN L P++ + P+SFL ST RS+S + + SN
Sbjct: 2 ATAFAPTKLTATVPLHG---SHENRLLLPIRLAPPSSFLGST---RSLSLRRLNH-SNAT 54
Query: 62 SRSSIHAVSDVLKEKKIKSNSSLPNLLITKEEGLELYEDMILGRAFEDMCAQMYYRGKMF 121
RS + +V +V+KEK+ +N+SL LITKEEGLELYEDMILGR+FEDMCAQMYYRGKMF
Sbjct: 55 RRSPVVSVQEVVKEKQSTNNTSL---LITKEEGLELYEDMILGRSFEDMCAQMYYRGKMF 111
Query: 122 GFVHLYNGQEAVSTGFIKLLKQEDSVVSTYRDHVHALSKGVPARQVMSELFGKATGCCRG 181
GFVHLYNGQEAVSTGFIKLL + DSVVSTYRDHVHALSKGV AR VMSELFGK TGCCRG
Sbjct: 112 GFVHLYNGQEAVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRG 171
Query: 182 QGGSMHMFSKEHNVLGGFAFIGEGIPVATGAAFTSKYKREVLKEADCDHVTCAFFGDGTC 241
QGGSMHMFSKEHN+LGGFAFIGEGIPVATGAAF+SKY+REVLK+ DCD VT AFFGDGTC
Sbjct: 172 QGGSMHMFSKEHNMLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTC 230
Query: 242 NNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGM 301
NNGQFFECLNMAAL+KLPI+FVVENNLWAIGMSHLR+TSDPEIWKKGPAFGMPGVHVDGM
Sbjct: 231 NNGQFFECLNMAALYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGM 290
Query: 302 DVLKVREVAKEAIGRARRGEGPTLIECETYRFRGHSLADPDELRDPDEKSHYGARDPIKA 361
DVLKVREVAKEA+ RARRGEGPTL+ECETYRFRGHSLADPDELRD EK+ Y ARDPI A
Sbjct: 291 DVLKVREVAKEAVTRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAA 350
Query: 362 LKNYMIENNLVSEVELKAIEKKIDEVIEESVEFADASPHPQRSQLLENVFADPKGFGIGP 421
LK Y+IEN L E ELK+IEKKIDE++EE+VEFADASP P RSQLLENVFADPKGFGIGP
Sbjct: 351 LKKYLIENKLAKEAELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGP 410
Query: 422 DGKYRCEDPKFTEGTAHV 439
DG+YRCEDPKFTEGTA V
Sbjct: 411 DGRYRCEDPKFTEGTAQV 428
>AT1G59900.1 | pyruvate dehydrogenase complex E1 alpha subunit |
Chr1:22051368-22053660 FORWARD LENGTH=389 | 201606
Length = 389
Score = 247 bits (631), Expect = 6e-78, Method: Compositional matrix adjust.
Identities = 133/341 (39%), Positives = 200/341 (58%), Gaps = 19/341 (5%)
Query: 90 TKEEGLELYEDMILGRAFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLKQEDSVV 148
+ +E L+ + M L R E + A Y+ K+ GF HLY+GQEAV+ G + ++D+++
Sbjct: 55 SSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAII 113
Query: 149 STYRDHVHALSKGVPARQVMSELFGKATGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIPV 208
+ YRDH L +G +V SEL G+ GC +G+GGSMH + KE + GG +G +P+
Sbjct: 114 TAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPL 173
Query: 209 ATGAAFTSKYKREVLKEADCDHVTCAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 268
G AF KY +E + VT A +GDG N GQ FE LN++ALW LP + V ENN
Sbjct: 174 GCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNH 226
Query: 269 WAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLIEC 328
+ +G + R+ P +K+G +PG+ VDGMD V++ K A A +GP ++E
Sbjct: 227 YGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-KGPIILEM 283
Query: 329 ETYRFRGHSLADP-DELRDPDEKSHY-GARDPIKALKNYMIENNLVSEVELKAIEKKIDE 386
+TYR+ GHS++DP R DE S RDPI+ +K ++ ++L +E ELK +EK+I +
Sbjct: 284 DTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRK 343
Query: 387 VIEESVEFADASPHPQRSQLLENVFADPKGFG---IGPDGK 424
+++++ A P P+ S+L NV+ KGFG GPD K
Sbjct: 344 EVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382
>AT1G59900.2 | pyruvate dehydrogenase complex E1 alpha subunit |
Chr1:22051368-22053537 FORWARD LENGTH=378 | 201606
Length = 378
Score = 244 bits (622), Expect = 1e-76, Method: Compositional matrix adjust.
Identities = 129/332 (38%), Positives = 196/332 (59%), Gaps = 16/332 (4%)
Query: 90 TKEEGLELYEDMILGRAFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLKQEDSVV 148
+ +E L+ + M L R E + A Y+ K+ GF HLY+GQEAV+ G + ++D+++
Sbjct: 55 SSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAII 113
Query: 149 STYRDHVHALSKGVPARQVMSELFGKATGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIPV 208
+ YRDH L +G +V SEL G+ GC +G+GGSMH + KE + GG +G +P+
Sbjct: 114 TAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPL 173
Query: 209 ATGAAFTSKYKREVLKEADCDHVTCAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 268
G AF KY +E + VT A +GDG N GQ FE LN++ALW LP + V ENN
Sbjct: 174 GCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNH 226
Query: 269 WAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLIEC 328
+ +G + R+ P +K+G +PG+ VDGMD V++ K A A +GP ++E
Sbjct: 227 YGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-KGPIILEM 283
Query: 329 ETYRFRGHSLADP-DELRDPDEKSHY-GARDPIKALKNYMIENNLVSEVELKAIEKKIDE 386
+TYR+ GHS++DP R DE S RDPI+ +K ++ ++L +E ELK +EK+I +
Sbjct: 284 DTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRK 343
Query: 387 VIEESVEFADASPHPQRSQLLENVFADPKGFG 418
+++++ A P P+ S+L NV+ KGFG
Sbjct: 344 EVDDAIAKAKDCPMPEPSELFTNVYV--KGFG 373
>AT1G24180.1 | Thiamin diphosphate-binding fold (THDP-binding)
superfamily protein | Chr1:8560777-8563382 REVERSE
LENGTH=393 | 201606
Length = 393
Score = 237 bits (604), Expect = 8e-74, Method: Compositional matrix adjust.
Identities = 128/339 (37%), Positives = 195/339 (57%), Gaps = 15/339 (4%)
Query: 90 TKEEGLELYEDMILGRAFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLKQEDSVV 148
+ EE L + DM R E + A Y+ K+ GF HLY+GQEA++ G + ++D+++
Sbjct: 59 SSEEILAFFRDMARMRRME-IAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAII 117
Query: 149 STYRDHVHALSKGVPARQVMSELFGKATGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIPV 208
++YRDH + +G SEL G+ TGC G+GGSMH + K+ + GG +G IP+
Sbjct: 118 TSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPL 177
Query: 209 ATGAAFTSKYKREVLKEADCDHVTCAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 268
G AF KY ++ + VT A +GDG N GQ FE LN++ALW LP + V ENN
Sbjct: 178 GCGLAFAQKYNKD-------EAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNH 230
Query: 269 WAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLIEC 328
+ +G + RS P +K+G +PG+ VDGMD L V++ K A A + GP ++E
Sbjct: 231 YGMGTATWRSAKSPAYFKRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEM 287
Query: 329 ETYRFRGHSLADP-DELRDPDEKSHY-GARDPIKALKNYMIENNLVSEVELKAIEKKIDE 386
+TYR+ GHS++DP R DE S RDPI+ ++ ++ +++ +E ELK +EK+I +
Sbjct: 288 DTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRK 347
Query: 387 VIEESVEFADASPHPQRSQLLENVFADPKGF-GIGPDGK 424
++++V A SP P S+L N++ G G D K
Sbjct: 348 EVDDAVAQAKESPIPDASELFTNMYVKDCGVESFGADRK 386
>AT5G09300.2 | Thiamin diphosphate-binding fold (THDP-binding)
superfamily protein | Chr5:2884282-2886291 REVERSE
LENGTH=401 | 201606
Length = 401
Score = 123 bits (308), Expect = 7e-31, Method: Compositional matrix adjust.
Identities = 84/327 (25%), Positives = 153/327 (46%), Gaps = 14/327 (4%)
Query: 89 ITKEEGLELYEDMILGRAFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLKQEDSVV 148
+++E +++Y DM+ + +++ + +G++ F G+EA++ L +D +
Sbjct: 54 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 112
Query: 149 STYRDHVHALSKGVPARQVMSELFGKATGCCRGQGGSMHMFSKEHNVLGGFAFIGEGIPV 208
YR+ L +G ++ ++ FG + +G+ +H S + N A I +P
Sbjct: 113 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 172
Query: 209 ATGAAFTSKYKREVLKEADCDHVTCAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 268
A GAA++ K ++ D +FGDG + G F LN+AA+ + P++F+ NN
Sbjct: 173 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 225
Query: 269 WAIGMSHLRSTSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLIEC 328
WAI + KG A+G+ + VDG D L + A A R + P LIE
Sbjct: 226 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 285
Query: 329 ETYRFRGHSLADPD-ELRDPDEKSHYG-ARDPIKALKNYMIENNLVSEVELKAIEKKIDE 386
TYR HS +D R E + AR+P+ + ++ N S+ + +I +
Sbjct: 286 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 345
Query: 387 VIEESVEFADASPHPQRSQLLENVFAD 413
+ E++ A+ + P L+N+F+D
Sbjct: 346 EMLEALRVAEKTEKPN----LQNMFSD 368