BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000108.1_g1410.1
(614 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G33220.1 | pectin methylesterase 44 | Chr4:16022506-16026130 ... 566 0.0
AT3G43270.1 | Plant invertase/pectin methylesterase inhibitor su... 548 0.0
AT4G33220.2 | pectin methylesterase 44 | Chr4:16024445-16026130 ... 496 e-172
AT3G05620.1 | Plant invertase/pectin methylesterase inhibitor su... 409 e-136
AT3G49220.1 | Plant invertase/pectin methylesterase inhibitor su... 405 e-134
>AT4G33220.1 | pectin methylesterase 44 | Chr4:16022506-16026130
FORWARD LENGTH=525 | 201606
Length = 525
Score = 566 bits (1459), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 299/581 (51%), Positives = 380/581 (65%), Gaps = 81/581 (13%)
Query: 34 ECLNTPTSIFLSSVELTIEQVNKVTLLVSKFSNVFAHSFRLSNAINDCLDLLDLSAEELR 93
+ L P S F+SS+ + + +V+ ++S+F++ F+ RL NA++DCLDLLD S+EEL
Sbjct: 26 DYLKVPASEFVSSINTIVVVIRQVSSILSQFAD-FSGDRRLQNAVSDCLDLLDFSSEELT 84
Query: 94 WTLASLSSASASHHKNHKTAEGRPTQKRGKAGRLLSDDLRSWLSAALDNQDTCIQGFENT 153
W SASAS + P K G + SD R+WLSAAL NQ TC++GF+ T
Sbjct: 85 W------SASASEN---------PKGKGNGTGDVGSD-TRTWLSAALSNQATCMEGFDGT 128
Query: 154 TGFVYERRLMSEALKHVTSLVCHLLSLVRHENQTNRYHQSPQQKGPSSGSGSGFDSRTSR 213
+G V + L++ +L + S++ LL LV+ P+QK
Sbjct: 129 SGLV--KSLVAGSLDQLYSMLRELLPLVQ-----------PEQK---------------- 159
Query: 214 DSVPSWMHIRDRKLLDQAPPSLPPPPPAAPSLLPPPPPPPPPPSLPFTPPPPPPPSPSPP 273
P ++ P P A P PP L T P
Sbjct: 160 ------------------PKAVSKPGPIAKG-----PKAPPGRKLRDTDEDESLQFPDWV 196
Query: 274 PPPPPPFNNGSSTTNNSSIVADVVVAADGSGNFTRIMDAVLAAPNNSTSRYIIYIKRGIY 333
P + T DV VA DG+GNFT+IMDA+ AP+ S++R++IYIK+G+Y
Sbjct: 197 RPDDRKLLESNGRT------YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLY 250
Query: 334 KENVEIKKKKWNLMMIGDGMDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDLSIENT 393
ENVEIKKKKWN++M+GDG+D TVISGNRSFIDGWTTFRSATFAVSGRGF+ARD++ +NT
Sbjct: 251 LENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNT 310
Query: 394 AGPAKHQAVALRSDSDLSVFFRCGINGYQDTLYAHSLRQFYRECRITGTVDFIFGNGAAV 453
AGP KHQAVALRSDSDLSVFFRC + GYQDTLY H++RQFYREC ITGTVDFIFG+G V
Sbjct: 311 AGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVV 370
Query: 454 FQNCNILARKPLQDQKNTITAQGRKDPNQNTGFSIQFSNITADLDLAADSVAFPNSSTTQ 513
FQNC ILA++ L +QKNTITAQGRKD NQ +GFSIQFSNI+AD DL P +TT+
Sbjct: 371 FQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLV------PYLNTTR 424
Query: 514 TFLGRPWKEYSRTVIMQSYIGSVIRPEGWLEWQGNFALDTLYYGEYMNYGPGAGLGSRVQ 573
T+LGRPWK YSRTV +++ + V+RPEGWLEW +FALDTL+YGE+MNYGPG+GL SRV+
Sbjct: 425 TYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVK 484
Query: 574 WPGFHAINDSTQAETFTVAQFIDGNTWLPYTGIKFTAGLVL 614
WPG+H N+S QA FTV+QFI GN WLP TG+ F+ GL +
Sbjct: 485 WPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGLYI 525
>AT3G43270.1 | Plant invertase/pectin methylesterase inhibitor
superfamily | Chr3:15222402-15225124 REVERSE LENGTH=527
| 201606
Length = 527
Score = 548 bits (1411), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 289/593 (48%), Positives = 367/593 (61%), Gaps = 93/593 (15%)
Query: 22 EDSIEDDDFLGTECLNTPTSIFLSSVELTIEQVNKVTLLVSKFSNVFAHSFRLSNAINDC 81
+++ D + ECL P F + + ++ + K +VSKF S R+SNAI DC
Sbjct: 26 KEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKS-RVSNAIVDC 84
Query: 82 LDLLDLSAEELRWTLASLSSASASHHKNHKTAEGRPTQKRGKAGRLLSDDLRSWLSAALD 141
+DLLD +AEEL W + S++ + + K++ T + + DLR+W+SAAL
Sbjct: 85 VDLLDSAAEELSWII---SASQSPNGKDNSTGD-------------VGSDLRTWISAALS 128
Query: 142 NQDTCIQGFENTTGFVYERRLMSEALKHVTSLVCHLLSLVRHENQTNRYHQSPQQKGPSS 201
NQDTC+ GFE T G + +++++ L V + V +LL++V + Q +
Sbjct: 129 NQDTCLDGFEGTNGII--KKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKA 186
Query: 202 GSG-SGFDSRTSRDSVPSWMHIRDRKLLDQAPPSLPPPPPAAPSLLPPPPPPPPPPSLPF 260
SG S F PSW+ DRKLL ++ AA
Sbjct: 187 HSGFSKF---------PSWVKPGDRKLLQTDNITVADAVVAADG---------------- 221
Query: 261 TPPPPPPPSPSPPPPPPPPFNNGSSTTNNSSIVADVVVAADGSGNFTRIMDAVLAAPNNS 320
T N + ++D AVLAAP+ S
Sbjct: 222 --------------------------TGNFTTISD----------------AVLAAPDYS 239
Query: 321 TSRYIIYIKRGIYKENVEIKKKKWNLMMIGDGMDATVISGNRSFIDGWTTFRSATFAVSG 380
T RY+I++KRG+Y ENVEIKKKKWN+MM+GDG+DATVI+GNRSFIDGWTTFRSATFAVSG
Sbjct: 240 TKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSG 299
Query: 381 RGFIARDLSIENTAGPAKHQAVALRSDSDLSVFFRCGINGYQDTLYAHSLRQFYRECRIT 440
RGFIARD++ +NTAGP KHQAVA+RSD+DL VF+RC + GYQDTLYAHS+RQF+REC IT
Sbjct: 300 RGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIIT 359
Query: 441 GTVDFIFGNGAAVFQNCNILARKPLQDQKNTITAQGRKDPNQNTGFSIQFSNITADLDLA 500
GTVDFIFG+ AVFQ+C I A++ L +QKN+ITAQGRKDPN+ TGF+IQFSNI AD DL
Sbjct: 360 GTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDL- 418
Query: 501 ADSVAFPNSSTTQTFLGRPWKEYSRTVIMQSYIGSVIRPEGWLEWQGNFALDTLYYGEYM 560
N +TT T+LGRPWK YSRTV MQ+Y+ I P GWLEW GNFALDTLYYGEYM
Sbjct: 419 -----LLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYM 473
Query: 561 NYGPGAGLGSRVQWPGFHAINDSTQAETFTVAQFIDGNTWLPYTGIKFTAGLV 613
N GPGA L RV+WPG+H +N S +A FTV+Q I GN WLP TGI F AGLV
Sbjct: 474 NSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGLV 526
>AT4G33220.2 | pectin methylesterase 44 | Chr4:16024445-16026130
FORWARD LENGTH=404 | 201606
Length = 404
Score = 496 bits (1278), Expect = e-172, Method: Compositional matrix adjust.
Identities = 239/390 (61%), Positives = 289/390 (74%), Gaps = 12/390 (3%)
Query: 225 RKLLDQAPPSLPPPPPAAPSLLPPPPPPPPPPSLPFTPPPPPPPSPSPPPPPPPPFNNGS 284
R+LL P P + P + P PP L T P P +
Sbjct: 27 RELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKLLESN 86
Query: 285 STTNNSSIVADVVVAADGSGNFTRIMDAVLAAPNNSTSRYIIYIKRGIYKENVEIKKKKW 344
T DV VA DG+GNFT+IMDA+ AP+ S++R++IYIK+G+Y ENVEIKKKKW
Sbjct: 87 GRT------YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKW 140
Query: 345 NLMMIGDGMDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDLSIENTAGPAKHQAVAL 404
N++M+GDG+D TVISGNRSFIDGWTTFRSATFAVSGRGF+ARD++ +NTAGP KHQAVAL
Sbjct: 141 NIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVAL 200
Query: 405 RSDSDLSVFFRCGINGYQDTLYAHSLRQFYRECRITGTVDFIFGNGAAVFQNCNILARKP 464
RSDSDLSVFFRC + GYQDTLY H++RQFYREC ITGTVDFIFG+G VFQNC ILA++
Sbjct: 201 RSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRG 260
Query: 465 LQDQKNTITAQGRKDPNQNTGFSIQFSNITADLDLAADSVAFPNSSTTQTFLGRPWKEYS 524
L +QKNTITAQGRKD NQ +GFSIQFSNI+AD DL P +TT+T+LGRPWK YS
Sbjct: 261 LPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLV------PYLNTTRTYLGRPWKLYS 314
Query: 525 RTVIMQSYIGSVIRPEGWLEWQGNFALDTLYYGEYMNYGPGAGLGSRVQWPGFHAINDST 584
RTV +++ + V+RPEGWLEW +FALDTL+YGE+MNYGPG+GL SRV+WPG+H N+S
Sbjct: 315 RTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSD 374
Query: 585 QAETFTVAQFIDGNTWLPYTGIKFTAGLVL 614
QA FTV+QFI GN WLP TG+ F+ GL +
Sbjct: 375 QANNFTVSQFIKGNLWLPSTGVTFSDGLYI 404
>AT3G05620.1 | Plant invertase/pectin methylesterase inhibitor
superfamily | Chr3:1629658-1631766 REVERSE LENGTH=543 |
201606
Length = 543
Score = 409 bits (1052), Expect = e-136, Method: Compositional matrix adjust.
Identities = 193/318 (60%), Positives = 234/318 (73%), Gaps = 16/318 (5%)
Query: 295 DVVVAADGSGNFTRIMDAVLAAPNNSTSRYIIYIKRGIYKENVEIKKKKWNLMMIGDGMD 354
+ VVA DG G + I +A+ APN+ST RY+IY+K+G+YKEN+++KKKK N+M++GDG+
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299
Query: 355 ATVISGNRSFIDGWTTFRSATFAVSGRGFIARDLSIENTAGPAKHQAVALRSDSDLSVFF 414
T+I+G+R+F+ G TTFR+AT AVSGRGFIA+D++ NTAGP QAVALR DSD S F+
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFY 359
Query: 415 RCGINGYQDTLYAHSLRQFYRECRITGTVDFIFGNGAAVFQNCNILARKPLQDQKNTITA 474
RC + GYQDTLYAHSLRQFYR+C I GT+DFIFGNGAAV QNC I R PL QK TITA
Sbjct: 360 RCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITA 419
Query: 475 QGRKDPNQNTGFSIQFSNITADLDLAADSVAFPNSSTTQTFLGRPWKEYSRTVIMQSYIG 534
QGRK PNQNTGF IQ S + A T T+LGRPWK YSRTV M +Y+
Sbjct: 420 QGRKSPNQNTGFVIQNSYVLA---------------TQPTYLGRPWKLYSRTVYMNTYMS 464
Query: 535 SVIRPEGWLEWQGNFALDTLYYGEYMNYGPGAGLGSRVQWPGFHAINDSTQAETFTVAQF 594
+++P GWLEW GNFALDTL+YGEY N GPG RV+WPG+H I D A +FTV F
Sbjct: 465 QLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYH-IMDKRTALSFTVGSF 523
Query: 595 IDGNTWLPYTGIKFTAGL 612
IDG WLP TG+ FTAGL
Sbjct: 524 IDGRRWLPATGVTFTAGL 541
Score = 64.3 bits (155), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 46/152 (30%), Positives = 73/152 (48%), Gaps = 21/152 (13%)
Query: 39 PTSIFLSSVELTIEQVNKVTLLVSKFSNVFAHSFRLSN--AINDCLDLLDLSAEELRWTL 96
P S+ ++V+ E +K L + + V S R AI DC +L+ S EL W++
Sbjct: 67 PHSVLRAAVK---EAHDKAKLAMERIPTVMMLSIRSREQVAIEDCKELVGFSVTELAWSM 123
Query: 97 ASLSSASASHHKNHKTAEGRPTQKRGKAGRLLSDDLRSWLSAALDNQDTCIQGFENTTGF 156
++ H + AG +L++WLSAA+ NQDTC++GFE T
Sbjct: 124 LEMNKL----HGGGGIDLDDGSHDAAAAG----GNLKTWLSAAMSNQDTCLEGFEGT--- 172
Query: 157 VYERR---LMSEALKHVTSLVCHLLSLVRHEN 185
ER+ L+ +L+ VT LV ++L + N
Sbjct: 173 --ERKYEELIKGSLRQVTQLVSNVLDMYTQLN 202
>AT3G49220.1 | Plant invertase/pectin methylesterase inhibitor
superfamily | Chr3:18249840-18253647 FORWARD LENGTH=598
| 201606
Length = 598
Score = 405 bits (1042), Expect = e-134, Method: Compositional matrix adjust.
Identities = 195/324 (60%), Positives = 235/324 (72%), Gaps = 7/324 (2%)
Query: 290 SSIVADVVVAADGSGNFTRIMDAVLAAPNNSTSRYIIYIKRGIYKEN-VEIKKKKWNLMM 348
S I AD++V+ DG+G I +A+ AP NST R IIY+K G Y+EN +++ +KK NLM
Sbjct: 279 SQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMF 338
Query: 349 IGDGMDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDLSIENTAGPAKHQAVALRSDS 408
+GDG TVISG +S D TTF +A+FA +G GFIARD++ EN AGPAKHQAVALR +
Sbjct: 339 VGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGA 398
Query: 409 DLSVFFRCGINGYQDTLYAHSLRQFYRECRITGTVDFIFGNGAAVFQNCNILARKPLQDQ 468
D +V +RC I GYQDTLY HS RQF+REC I GTVDFIFGN A V QNC+I ARKP+ Q
Sbjct: 399 DHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQ 458
Query: 469 KNTITAQGRKDPNQNTGFSIQFSNITADLDLAADSVAFPNSSTTQTFLGRPWKEYSRTVI 528
KNTITAQ RKDPNQNTG SI S + A DL A + +TQT+LGRPWK +SRTV
Sbjct: 459 KNTITAQNRKDPNQNTGISIHASRVLAASDLQA------TNGSTQTYLGRPWKLFSRTVY 512
Query: 529 MQSYIGSVIRPEGWLEWQGNFALDTLYYGEYMNYGPGAGLGSRVQWPGFHAINDSTQAET 588
M SYIG + GWLEW FALDTLYYGEY+N GPG+GLG RV WPG+ IN + +A
Sbjct: 513 MMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANR 572
Query: 589 FTVAQFIDGNTWLPYTGIKFTAGL 612
FTVA+FI G++WLP TG+ F AGL
Sbjct: 573 FTVAEFIYGSSWLPSTGVSFLAGL 596
Score = 48.5 bits (114), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 43/158 (27%), Positives = 74/158 (46%), Gaps = 32/158 (20%)
Query: 73 RLSNAINDCLDLLDLSAEELRWTLASLSSASASHHKNHKTAEGRPTQKRGKAGRLLSDDL 132
R +A + C++LLD S + L L+S+ S+SA +P D+
Sbjct: 149 RARSAYDSCVELLDDSVDALSRALSSVVSSSA-----------KP------------QDV 185
Query: 133 RSWLSAALDNQDTCIQGFENT-TGFVYERRLMSEALKHVTSLVCHLLSLVRHENQTNRYH 191
+WLSAAL N DTC +GF+ G V + M+ AL++++ LV + L++ + + +
Sbjct: 186 TTWLSAALTNHDTCTEGFDGVDDGGVKDH--MTAALQNLSELVSNCLAIFSASHDGDDFA 243
Query: 192 QSPQQKGPSSGSGSGFDSRTSRDSVPSWMHIRDRKLLD 229
P Q G + P WM ++R++L+
Sbjct: 244 GVPIQNRRLLG------VEEREEKFPRWMRPKEREILE 275