BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000108.1_g3060.1
(625 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G17000.3 | neurofilament heavy protein | Chr4:9567046-9569913... 187 8e-51
AT4G17000.1 | neurofilament heavy protein | Chr4:9567046-9569913... 187 8e-51
AT4G17000.2 | neurofilament heavy protein | Chr4:9567046-9569393... 93 1e-19
>AT4G17000.3 | neurofilament heavy protein | Chr4:9567046-9569913
REVERSE LENGTH=661 | 201606
Length = 661
Score = 187 bits (474), Expect = 8e-51, Method: Compositional matrix adjust.
Identities = 165/445 (37%), Positives = 239/445 (53%), Gaps = 57/445 (12%)
Query: 1 MEKFDELVPNFDVTVLSNDSDDDEIYEKIEAPKFVDLTAPDKSLQVDDRSWFCLRVGCDL 60
ME F+ ++ ++ ++++ YE IEAPKFVDLTAPD + DDR WFC RVGCD
Sbjct: 1 MEAFEHMI--------VDEFNEEDFYETIEAPKFVDLTAPDHRPEGDDRYWFCSRVGCDQ 52
Query: 61 KHEEEIDPETIYKQFVLRVMAARSPNFRFRKTLSRQ-ASANMKCPLSAPAKSSKRRMSRL 119
KHEE +D E IYK+FVLRVMAARSP+ R RK L R+ S + KCP + PAK S+ R+S+L
Sbjct: 53 KHEEFLDSEAIYKKFVLRVMAARSPSVRLRKALYRKDFSVDPKCPNTVPAKPSRSRVSKL 112
Query: 120 TVVTSLSQK----LADAKVKVHPLCKLNSTPKEKAKK------SSVAAKALTTPRNKKCL 169
+++S+ QK + +VKV K N TPK KAK SSV KALT KK +
Sbjct: 113 AMISSIPQKGNGNIRSKEVKVVSTNK-NVTPKAKAKGKESAVISSVPQKALT--ERKKQM 169
Query: 170 PSQDPFRSVQNPK-AKPAIPRNRRVAKSL-FGSPKK------AVESQTPGTDICSGMKKL 221
S FRSVQNP+ A + NR VAK+L F SPKK +VE + +C+GM+KL
Sbjct: 170 QSPAAFRSVQNPRNATIKVSENRVVAKALVFQSPKKLVKLKRSVELSSSVKKLCNGMRKL 229
Query: 222 EINDQSNRPCELAGRTPRGPDKRVTASAE---PLESK-LRNQTSNN-RTKLSRDHKGKPK 276
EI+++ R G + +V +SA PL+++ ++++ ++ R++ D K K
Sbjct: 230 EIDNK---------RNGLGVNHKVVSSASSRRPLKTREVKSRVFDSLRSQKQIDQKDKGV 280
Query: 277 SLRRSKSKSKGNLRKSITVSPQHGPID--DFSDMEI-DQKSRKGSINV--CSEEKIAVED 331
S + + K K + P P+ D + ME+ D+ SR + V SEE
Sbjct: 281 STLKKRVKKKED------PVPSSDPLKPYDSNGMEVEDKTSRDEELLVENKSEELSDTSK 334
Query: 332 DNTIDIMQPV--PSTEKDSEEKDCHKSETSELEIERSNEEIQPKDMMKSNLGELILEEKL 389
N + +Q P+ K+S K + +E+E + S + +D +N+ I +E +
Sbjct: 335 ANMNNQLQAREDPAVIKESGLATSQKYQITEIEEKESALASECEDKENANIVAAIDKEDI 394
Query: 390 YPISHPSSRKLYPISPTEIDDKENA 414
I K EI+DKENA
Sbjct: 395 AVIKVSGLDKAKQCETVEIEDKENA 419
>AT4G17000.1 | neurofilament heavy protein | Chr4:9567046-9569913
REVERSE LENGTH=674 | 201606
Length = 674
Score = 187 bits (474), Expect = 8e-51, Method: Compositional matrix adjust.
Identities = 165/445 (37%), Positives = 239/445 (53%), Gaps = 57/445 (12%)
Query: 1 MEKFDELVPNFDVTVLSNDSDDDEIYEKIEAPKFVDLTAPDKSLQVDDRSWFCLRVGCDL 60
ME F+ ++ ++ ++++ YE IEAPKFVDLTAPD + DDR WFC RVGCD
Sbjct: 1 MEAFEHMI--------VDEFNEEDFYETIEAPKFVDLTAPDHRPEGDDRYWFCSRVGCDQ 52
Query: 61 KHEEEIDPETIYKQFVLRVMAARSPNFRFRKTLSRQ-ASANMKCPLSAPAKSSKRRMSRL 119
KHEE +D E IYK+FVLRVMAARSP+ R RK L R+ S + KCP + PAK S+ R+S+L
Sbjct: 53 KHEEFLDSEAIYKKFVLRVMAARSPSVRLRKALYRKDFSVDPKCPNTVPAKPSRSRVSKL 112
Query: 120 TVVTSLSQK----LADAKVKVHPLCKLNSTPKEKAKK------SSVAAKALTTPRNKKCL 169
+++S+ QK + +VKV K N TPK KAK SSV KALT KK +
Sbjct: 113 AMISSIPQKGNGNIRSKEVKVVSTNK-NVTPKAKAKGKESAVISSVPQKALT--ERKKQM 169
Query: 170 PSQDPFRSVQNPK-AKPAIPRNRRVAKSL-FGSPKK------AVESQTPGTDICSGMKKL 221
S FRSVQNP+ A + NR VAK+L F SPKK +VE + +C+GM+KL
Sbjct: 170 QSPAAFRSVQNPRNATIKVSENRVVAKALVFQSPKKLVKLKRSVELSSSVKKLCNGMRKL 229
Query: 222 EINDQSNRPCELAGRTPRGPDKRVTASAE---PLESK-LRNQTSNN-RTKLSRDHKGKPK 276
EI+++ R G + +V +SA PL+++ ++++ ++ R++ D K K
Sbjct: 230 EIDNK---------RNGLGVNHKVVSSASSRRPLKTREVKSRVFDSLRSQKQIDQKDKGV 280
Query: 277 SLRRSKSKSKGNLRKSITVSPQHGPID--DFSDMEI-DQKSRKGSINV--CSEEKIAVED 331
S + + K K + P P+ D + ME+ D+ SR + V SEE
Sbjct: 281 STLKKRVKKKED------PVPSSDPLKPYDSNGMEVEDKTSRDEELLVENKSEELSDTSK 334
Query: 332 DNTIDIMQPV--PSTEKDSEEKDCHKSETSELEIERSNEEIQPKDMMKSNLGELILEEKL 389
N + +Q P+ K+S K + +E+E + S + +D +N+ I +E +
Sbjct: 335 ANMNNQLQAREDPAVIKESGLATSQKYQITEIEEKESALASECEDKENANIVAAIDKEDI 394
Query: 390 YPISHPSSRKLYPISPTEIDDKENA 414
I K EI+DKENA
Sbjct: 395 AVIKVSGLDKAKQCETVEIEDKENA 419
>AT4G17000.2 | neurofilament heavy protein | Chr4:9567046-9569393
REVERSE LENGTH=590 | 201606
Length = 590
Score = 93.2 bits (230), Expect = 1e-19, Method: Compositional matrix adjust.
Identities = 122/366 (33%), Positives = 184/366 (50%), Gaps = 49/366 (13%)
Query: 80 MAARSPNFRFRKTLSRQ-ASANMKCPLSAPAKSSKRRMSRLTVVTSLSQK----LADAKV 134
MAARSP+ R RK L R+ S + KCP + PAK S+ R+S+L +++S+ QK + +V
Sbjct: 1 MAARSPSVRLRKALYRKDFSVDPKCPNTVPAKPSRSRVSKLAMISSIPQKGNGNIRSKEV 60
Query: 135 KVHPLCKLNSTPKEKAKK------SSVAAKALTTPRNKKCLPSQDPFRSVQNPK-AKPAI 187
KV K N TPK KAK SSV KALT KK + S FRSVQNP+ A +
Sbjct: 61 KVVSTNK-NVTPKAKAKGKESAVISSVPQKALT--ERKKQMQSPAAFRSVQNPRNATIKV 117
Query: 188 PRNRRVAKSL-FGSPKK------AVESQTPGTDICSGMKKLEINDQSNRPCELAGRTPRG 240
NR VAK+L F SPKK +VE + +C+GM+KLEI+++ R G
Sbjct: 118 SENRVVAKALVFQSPKKLVKLKRSVELSSSVKKLCNGMRKLEIDNK---------RNGLG 168
Query: 241 PDKRVTASAE---PLESK-LRNQTSNN-RTKLSRDHKGKPKSLRRSKSKSKGNLRKSITV 295
+ +V +SA PL+++ ++++ ++ R++ D K K S + + K K +
Sbjct: 169 VNHKVVSSASSRRPLKTREVKSRVFDSLRSQKQIDQKDKGVSTLKKRVKKKED------P 222
Query: 296 SPQHGPID--DFSDMEI-DQKSRKGSINV--CSEEKIAVEDDNTIDIMQPV--PSTEKDS 348
P P+ D + ME+ D+ SR + V SEE N + +Q P+ K+S
Sbjct: 223 VPSSDPLKPYDSNGMEVEDKTSRDEELLVENKSEELSDTSKANMNNQLQAREDPAVIKES 282
Query: 349 EEKDCHKSETSELEIERSNEEIQPKDMMKSNLGELILEEKLYPISHPSSRKLYPISPTEI 408
K + +E+E + S + +D +N+ I +E + I K EI
Sbjct: 283 GLATSQKYQITEIEEKESALASECEDKENANIVAAIDKEDIAVIKVSGLDKAKQCETVEI 342
Query: 409 DDKENA 414
+DKENA
Sbjct: 343 EDKENA 348