BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000112.1_g0360.1
(557 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G26260.1 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase i... 816 0.0
AT2G26260.2 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase i... 699 0.0
AT1G47290.2 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase i... 651 0.0
AT1G47290.1 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase i... 605 0.0
AT2G43420.1 | 3-beta hydroxysteroid dehydrogenase/isomerase fami... 412 e-138
>AT2G26260.1 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2 | Chr2:11178237-11182872 FORWARD LENGTH=564 |
201606
Length = 564
Score = 816 bits (2107), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 382/556 (68%), Positives = 458/556 (82%)
Query: 2 SREEKCCVVTGGRGFAARHLVEMLIESRQWVVRVADLGPTIKLEPYEEEGILGEALRLGG 61
+ E+ CVVTGGRGFAARHLVEML+ + VR+ADL P I L+P E G+L E LR G
Sbjct: 6 TETERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGR 65
Query: 62 AEYVSADLRDKAQVIKAFEGAEVVFHMAAPDSSINNQQLHYSVNVVGTKNVIDACVECKV 121
+Y+SADLRDK+QV+KAF+GAEVVFHMAAPDSSINN QL YSVNV GT+NVIDACV+ V
Sbjct: 66 VQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGV 125
Query: 122 KRLIYTSSPSVVFDGVHGIFNGDESLQYPPKHNDSYSATKAEGEGLVLKANGRDGLLTCC 181
KRLIYTSSPSVVFDGVHGI NG ES+ YP KHNDSYSATKAEGE L++KANGR+GLLTCC
Sbjct: 126 KRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCC 185
Query: 182 IRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNMYDFTYVENVAHAHVCAEKALASE 241
IRPSSIFGPGDRLLVPSLV AARAGKSKFIIGDGNN+YDFTYVENVAHAHVCAE+ALAS
Sbjct: 186 IRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASG 245
Query: 242 GALAERAAGQAFFITNTEPIKFWEFMSLILEGLGYERPSIKIPAPVMMPIAHLVELVYKV 301
G ++ +AAGQA+FITN EPIKFWEFMS +L+GLGYERPSIKIPA +MMPIAHLVEL YKV
Sbjct: 246 GDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKV 305
Query: 302 LGPYGMRVPQLTPSRIRLLSCSRTFNCSKAKDRIFYTPIVSLQEGLKRTIDSYQHLRAEH 361
LGPYGM VPQLTPSR+RLLSCSRTF+ +KAKDR+ Y P+V LQEG++RTIDS+ HL A
Sbjct: 306 LGPYGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGS 365
Query: 362 QPKREGPSKSHLFLGGGKVADTLLWKDKKRTISSLIVLVAFYYYFIASGYTLITAISKLL 421
Q KREGPSK+ LGGGKVADTLLWKD K+T+ ++ +L++ YY F+A+G T++TA+SK L
Sbjct: 366 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 425
Query: 422 MVTTLFLFVHGLLPTKIFGYAIEKIPPTSFHLPQERCNQIAIMAGSVWNSAVNDLQSLCK 481
+V ++FLF+HG+LP KIFGY +EKIP + FHL ++ + +++ S WN+ V L+SLC+
Sbjct: 426 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 485
Query: 482 GKDLTLFVKVCIFLLFLSFLGSTPIQTIFLIGIPLAFTAFVIYEKKEEEIDGLVHKALSF 541
G D + F KV LL LS G+ + +IF+IG+P+AF AF++YEKKE+EID +V SF
Sbjct: 486 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSF 545
Query: 542 GCKLKSDVSKKFVSSK 557
CK KSDV +K SK
Sbjct: 546 ACKHKSDVYEKLFGSK 561
>AT2G26260.2 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2 | Chr2:11178586-11182872 FORWARD LENGTH=473 |
201606
Length = 473
Score = 699 bits (1803), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 327/470 (69%), Positives = 391/470 (83%)
Query: 88 MAAPDSSINNQQLHYSVNVVGTKNVIDACVECKVKRLIYTSSPSVVFDGVHGIFNGDESL 147
MAAPDSSINN QL YSVNV GT+NVIDACV+ VKRLIYTSSPSVVFDGVHGI NG ES+
Sbjct: 1 MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESM 60
Query: 148 QYPPKHNDSYSATKAEGEGLVLKANGRDGLLTCCIRPSSIFGPGDRLLVPSLVTAARAGK 207
YP KHNDSYSATKAEGE L++KANGR+GLLTCCIRPSSIFGPGDRLLVPSLV AARAGK
Sbjct: 61 AYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGK 120
Query: 208 SKFIIGDGNNMYDFTYVENVAHAHVCAEKALASEGALAERAAGQAFFITNTEPIKFWEFM 267
SKFIIGDGNN+YDFTYVENVAHAHVCAE+ALAS G ++ +AAGQA+FITN EPIKFWEFM
Sbjct: 121 SKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFM 180
Query: 268 SLILEGLGYERPSIKIPAPVMMPIAHLVELVYKVLGPYGMRVPQLTPSRIRLLSCSRTFN 327
S +L+GLGYERPSIKIPA +MMPIAHLVEL YKVLGPYGM VPQLTPSR+RLLSCSRTF+
Sbjct: 181 SQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFD 240
Query: 328 CSKAKDRIFYTPIVSLQEGLKRTIDSYQHLRAEHQPKREGPSKSHLFLGGGKVADTLLWK 387
+KAKDR+ Y P+V LQEG++RTIDS+ HL A Q KREGPSK+ LGGGKVADTLLWK
Sbjct: 241 STKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWK 300
Query: 388 DKKRTISSLIVLVAFYYYFIASGYTLITAISKLLMVTTLFLFVHGLLPTKIFGYAIEKIP 447
D K+T+ ++ +L++ YY F+A+G T++TA+SK L+V ++FLF+HG+LP KIFGY +EKIP
Sbjct: 301 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 360
Query: 448 PTSFHLPQERCNQIAIMAGSVWNSAVNDLQSLCKGKDLTLFVKVCIFLLFLSFLGSTPIQ 507
+ FHL ++ + +++ S WN+ V L+SLC+G D + F KV LL LS G+ +
Sbjct: 361 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 420
Query: 508 TIFLIGIPLAFTAFVIYEKKEEEIDGLVHKALSFGCKLKSDVSKKFVSSK 557
+IF+IG+P+AF AF++YEKKE+EID +V SF CK KSDV +K SK
Sbjct: 421 SIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSDVYEKLFGSK 470
>AT1G47290.2 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 1 | Chr1:17336121-17339030 FORWARD LENGTH=439 |
201606
Length = 439
Score = 651 bits (1680), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 309/449 (68%), Positives = 372/449 (82%), Gaps = 18/449 (4%)
Query: 1 MSREEKCCVVTGGRGFAARHLVEMLIESRQWVVRVADLGPTIKLEPYEEEGILGEALRLG 60
++ E+ CVVTGGRGFAARHLVEML+ + + VR+ADL P I L P+EE GILGEA+R G
Sbjct: 5 VTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSG 64
Query: 61 GAEYVSADLRDKAQVIKAFEGAEVVFHMAAPDSSINNQQLHYSVNVVGTKNVIDACVECK 120
+YVSADLR+K QV+K F+GAEVVFHMAAPDSSINN QL YSVNV GT NVIDAC+E
Sbjct: 65 RVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVG 124
Query: 121 VKRLIYTSSPSVVFDGVHGIFNGDESLQYPPKHNDSYSATKAEGEGLVLKANGRDGLLTC 180
VKRLIYTSSPSVVFDGVHG N DESL YPPKHNDSYSATKAEGE L+LKANGR GLLTC
Sbjct: 125 VKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTC 184
Query: 181 CIRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNMYDFTYVENVAHAHVCAEKALAS 240
CIRPSSIFGPGD+L+VPSLVTAARAGKSKFIIGDG+N YDFTYVENV HAHVCAE+ALAS
Sbjct: 185 CIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALAS 244
Query: 241 EGALAERAAGQAFFITNTEPIKFWEFMSLILEGLGYERPSIKIPAPVMMPIAHLVELVYK 300
G + +AAGQA+FITN EPIKFWEFMS +LEGLGYERPSIKIPA +MMPIA+LVEL YK
Sbjct: 245 GGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYK 304
Query: 301 VLGPYGMRVPQLTPSRIRLLSCSRTFNCSKAKDRIFYTPIVSLQEGLKRTIDSYQHLRAE 360
+LGPYGM+VP LTPSR+RLLSC+RTF+ SKAKDR+ Y+P+V LQEG+KRTIDS+ HL+A+
Sbjct: 305 LLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364
Query: 361 HQPKREGPSKSHLFLGGGKVADTLLWKDKKRTISSLIVLVAFYYYFIA---SGYTLITAI 417
+QPK E V +T+ W KK+T+ ++++L+ Y+ F+A S +ITA+
Sbjct: 365 NQPKTE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAV 409
Query: 418 SKLLMVTTLFLFVHGLLPTKIFGYAIEKI 446
SK+L+V+++F+F++G+LP K+ + +KI
Sbjct: 410 SKVLLVSSIFMFINGILPEKMKVFGSKKI 438
>AT1G47290.1 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 1 | Chr1:17336121-17339030 FORWARD LENGTH=382 |
201606
Length = 382
Score = 605 bits (1560), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 283/366 (77%), Positives = 321/366 (87%)
Query: 1 MSREEKCCVVTGGRGFAARHLVEMLIESRQWVVRVADLGPTIKLEPYEEEGILGEALRLG 60
++ E+ CVVTGGRGFAARHLVEML+ + + VR+ADL P I L P+EE GILGEA+R G
Sbjct: 5 VTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSG 64
Query: 61 GAEYVSADLRDKAQVIKAFEGAEVVFHMAAPDSSINNQQLHYSVNVVGTKNVIDACVECK 120
+YVSADLR+K QV+K F+GAEVVFHMAAPDSSINN QL YSVNV GT NVIDAC+E
Sbjct: 65 RVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVG 124
Query: 121 VKRLIYTSSPSVVFDGVHGIFNGDESLQYPPKHNDSYSATKAEGEGLVLKANGRDGLLTC 180
VKRLIYTSSPSVVFDGVHG N DESL YPPKHNDSYSATKAEGE L+LKANGR GLLTC
Sbjct: 125 VKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTC 184
Query: 181 CIRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNMYDFTYVENVAHAHVCAEKALAS 240
CIRPSSIFGPGD+L+VPSLVTAARAGKSKFIIGDG+N YDFTYVENV HAHVCAE+ALAS
Sbjct: 185 CIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALAS 244
Query: 241 EGALAERAAGQAFFITNTEPIKFWEFMSLILEGLGYERPSIKIPAPVMMPIAHLVELVYK 300
G + +AAGQA+FITN EPIKFWEFMS +LEGLGYERPSIKIPA +MMPIA+LVEL YK
Sbjct: 245 GGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYK 304
Query: 301 VLGPYGMRVPQLTPSRIRLLSCSRTFNCSKAKDRIFYTPIVSLQEGLKRTIDSYQHLRAE 360
+LGPYGM+VP LTPSR+RLLSC+RTF+ SKAKDR+ Y+P+V LQEG+KRTIDS+ HL+A+
Sbjct: 305 LLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364
Query: 361 HQPKRE 366
+QPK E
Sbjct: 365 NQPKTE 370
>AT2G43420.1 | 3-beta hydroxysteroid dehydrogenase/isomerase family
protein | Chr2:18031493-18034936 REVERSE LENGTH=561 |
201606
Length = 561
Score = 412 bits (1058), Expect = e-138, Method: Compositional matrix adjust.
Identities = 223/527 (42%), Positives = 313/527 (59%), Gaps = 10/527 (1%)
Query: 6 KCCVVTGGRGFAARHLVEMLIESRQWVVRVADLGPTIKLEPYEEEGILGEALRLGGAEYV 65
K CVV GGRGF R LV L+ W VRVAD G T+ L+ E + +L +AL G A Y
Sbjct: 13 KTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRASYH 70
Query: 66 SADLRDKAQVIKAFEGAEVVFHMAAPDSSINNQQLHYSVNVVGTKNVIDACVECKVKRLI 125
D+RDK Q++K EG+ VVF+M A D ++ Y V V GT+NVI AC E V++LI
Sbjct: 71 CVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVRKLI 130
Query: 126 YTSSPSVVFDGVHGIFNGDESLQYPPKHNDSYSATKAEGEGLVLKANGRDGLLTCCIRPS 185
Y S+ VVFDG I +GDESL+ P K + KA+ E L+ AN RDGLLTC +R S
Sbjct: 131 YNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCALRSS 190
Query: 186 SIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNMYDFTYVENVAHAHVCAEKALASEGALA 245
+FGPGD VP LV A++G +KFI+G G N+ DFTY ENV+HAH+CA KAL S+
Sbjct: 191 IVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ---M 247
Query: 246 ERAAGQAFFITNTEPIKFWEFMSLILEGLGYERPSIKIPAPVMMPIAHLVELVYKVLGPY 305
E AG+ FFITN +P++FW+F+S I+EGLGY RPSIK+P +++ + L++ ++
Sbjct: 248 EFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE---KE 304
Query: 306 GMRVPQLTPSRIRLL-SCSRTFNCSKAKDRIFYTPIVSLQEGLKRTIDSYQHLRAEHQPK 364
G+ T + LL S +RTFNC+ AK + YTP+V+L++G+ T+ + E
Sbjct: 305 GLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEKSDD 363
Query: 365 REGPSKSHLFLGGGKVADTLLWKDKKRTISSLIVLVAFYYYFIASGYTLITAISKLLMVT 424
S + LG GKVAD LLW+++K+T S +VL FYY+F SG T ++ ++LL +
Sbjct: 364 TIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLLFIF 423
Query: 425 TLFLFVHGLLPTKIFGYAIEKIPPTSFHLPQERCNQIAIMAGSVWNSAVNDLQSLCKGKD 484
+ L+ +P+KIFG+ + KIPP F + + ++ VWN V +SL G D
Sbjct: 424 AVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSSGGD 483
Query: 485 LTLFVKVCIFLLFLSFLGSTPIQTIFLIGIPLAFTAFVIYEKKEEEI 531
F K+ L L + S + + +FT F IYE+ E E+
Sbjct: 484 WIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELEL 530