BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000118.1_g0600.1
(597 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G15810.1 | tRNA (guanine(26)-N(2))-dimethyltransferase | Chr5... 894 0.0
AT3G02320.1 | N2%2CN2-dimethylguanosine tRNA methyltransferase |... 888 0.0
AT3G56330.1 | N2%2CN2-dimethylguanosine tRNA methyltransferase |... 92 1e-19
>AT5G15810.1 | tRNA (guanine(26)-N(2))-dimethyltransferase |
Chr5:5158092-5161121 FORWARD LENGTH=593 | 201606
Length = 593
Score = 894 bits (2310), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 420/556 (75%), Positives = 476/556 (85%), Gaps = 4/556 (0%)
Query: 19 ISTDPNDYTIIKEGEAEILMHANNEVFYNKTQVNNRDLSIAVLRTYVAKRKEEHETMLSK 78
+ TD NDYT+IKEGEAE+LMH N+VF+NK QVNNRD+SIAVLR ++ KRK+EHE MLSK
Sbjct: 1 METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60
Query: 79 KTRKGKKVPSDEVFNGSVPEEITIQNGEKINGDCGAHVNAPQGEPDSISEKPIMITEGKG 138
+ R KV +V S EE +NG+ G H Q P E E
Sbjct: 61 RARSSGKVVEKDVSETS-KEETPTENGDDNGKTNGEHEVTTQDGP---KEAAKTAYESAR 116
Query: 139 RELKPTRVLEALAASGLRSLRYAREVEEIGQVVALDNDKASVEACRRNIKFNGSVTTAKV 198
RELKP RVLEAL+ASGLR+LRYAREVE IGQVVALDND ASVEAC+RNIKFNG ++T+KV
Sbjct: 117 RELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMSTSKV 176
Query: 199 ESHLTDARVFMLSHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGILMCTATDMAVLCGGN 258
ESHLTDARV MLSHPK+FDVVDLDPYG+PS+FLDSAVQSV DGG+LMCTATDMAVLCG N
Sbjct: 177 ESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGAN 236
Query: 259 GEVCYAKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRIF 318
GEVCY+KYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVP+LSVQMDFYVRVFVR++
Sbjct: 237 GEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVFVRVY 296
Query: 319 TSASEMKNSPLKLSYVYQCIGCDSFHLQCLARTVTKNNSVRYLPGFGPAVPQDCTDCGKK 378
TSAS MKN+PLKLSYVYQCIGCDSFHLQ + R++ KNNSVRYLPG GP VPQDCT CGKK
Sbjct: 297 TSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTHCGKK 356
Query: 379 FNVGGPIWSAPIHDQEWVTSILTNVRSLKERYPAYDRISAVLTTVSEELPDVPLFLSLHN 438
+N+GGPIWSAPIHDQEWV SIL V+S+K+RYPAYDRI AVLTT+SEELPDVPLFLSLH+
Sbjct: 357 YNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFLSLHS 416
Query: 439 LCATLKCTSPSAVMFRSAVLNAGYRISGTHVNPLGLKSDAPMEVIWDIMRCWIKNHPVKA 498
L ATLKCTSPSA +FRSAV+NA YR+SG+HVNPLG+K+DAPME+IWDIMRCW+KNHP+K
Sbjct: 417 LSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNHPIKP 476
Query: 499 QSPDQPGSVILSKEPTLQANFARAVASLSKAQAKKVARFLPNPEKHWGPKLRAGRTVTSK 558
QSP+ PGSVILSKEP+ QA+F+R V SLSKAQAKKVARFLPNPEKHWGPK+RAGRT+TSK
Sbjct: 477 QSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRTITSK 536
Query: 559 HVSLLGPRALNGSTND 574
HVSLLG A+NG N+
Sbjct: 537 HVSLLGHEAVNGHLNN 552
>AT3G02320.1 | N2%2CN2-dimethylguanosine tRNA methyltransferase |
Chr3:470097-473492 FORWARD LENGTH=589 | 201606
Length = 589
Score = 888 bits (2294), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 427/594 (71%), Positives = 488/594 (82%), Gaps = 23/594 (3%)
Query: 19 ISTDPNDYTIIKEGEAEILMHANNEVFYNKTQVNNRDLSIAVLRTYVAKRKEEHETMLSK 78
+ TD NDYT+IKEGEAEILMH N+VF+NK QVNNRD+SIAVLR +++KRK+EHE SK
Sbjct: 1 METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60
Query: 79 KTRKGKKVPSDEVFNGSVPEEITIQNGEKINGDCGAHVNAPQGEPDSISEKPIMITEGKG 138
+TR KV + S EE +NG NGD H A + P S+S+ P TE
Sbjct: 61 RTRPASKVIEKDASEAS-KEETPSENGMN-NGD---HEVASEDGPSSVSKDPAKTTERFA 115
Query: 139 -RELKPTRVLEALAASGLRSLRYAREVEEIGQVVALDNDKASVEACRRNIKFNGSVTTAK 197
RE KP +VLEAL+ASGLR+LRYARE+E IGQVVALDND ASVEAC+RNIKFNGSV +K
Sbjct: 116 PREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISK 175
Query: 198 VESHLTDARVFMLSHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGILMCTATDMAVLCGG 257
VESH TDARV ML+HPKEFDVVDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVLCGG
Sbjct: 176 VESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGG 235
Query: 258 NGEVCYAKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYVRVFVRI 317
NGEVCY+KYGSYPLR KYCHEMALRILLA IESHANRYKRYIVP+LSVQMDFYVRVFVR+
Sbjct: 236 NGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVFVRV 295
Query: 318 FTSASEMKNSPLKLSYVYQCIGCDSFHLQCLARTVTKNNSVRYLPGFGPAVPQDCTDCGK 377
+TSAS MKN+PLKLSYVYQCIGCDSFHLQ + R++ KNNSVRYLP GP V QDC CGK
Sbjct: 296 YTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNHCGK 355
Query: 378 KFNVGGPIWSAPIHDQEWVTSILTNVRSLKERYPAYDRISAVLTTVSEELPDVPLFLSLH 437
K+N+GGPIWSAP+HD EWVTSIL +V+S+K+RYPAYDRISAVLTTVSEEL DVPLFLSLH
Sbjct: 356 KYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFLSLH 415
Query: 438 NLCATLKCTSPSAVMFRSAVLNAGYRISGTHVNPLGLKSDAPMEVIWDIMRCWIKNHPVK 497
NLCATLKC SPSA MFRSAV+NA YRISGTHVNPLG+K+DAPMEVIWDIMRCW+KNHP+K
Sbjct: 416 NLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNHPIK 475
Query: 498 AQSPDQPGSVILSKEPTLQANFARAVASLSKAQAKKVARFLPNPEKHWGPKLRAGRTVTS 557
AQSP+QPGSVILSKEP+ + +F+R + SLSKAQAKKVARFLPNPEKHWGPKLRAGR +TS
Sbjct: 476 AQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQITS 535
Query: 558 KHVSLLGPRALNGSTNDQ-----------------EDGEEPDSKRQRTDDLTPT 594
KHVSL+G A+NG + +D +P ++ TD++T T
Sbjct: 536 KHVSLIGHEAVNGHLSQHHEELKEEDEEAEPEDNVQDKVDPKRQKTATDNITST 589
>AT3G56330.1 | N2%2CN2-dimethylguanosine tRNA methyltransferase |
Chr3:20890250-20891933 FORWARD LENGTH=433 | 201606
Length = 433
Score = 92.0 bits (227), Expect = 1e-19, Method: Compositional matrix adjust.
Identities = 90/354 (25%), Positives = 145/354 (40%), Gaps = 42/354 (11%)
Query: 145 RVLEALAASGLRSLRYAREVEEIGQVVALDNDKASVEACRRNIKFNGSVTTAKVESHLTD 204
RVL+A+ G+RSLRY E E + ND RR I N +KVE D
Sbjct: 91 RVLDAMCGCGIRSLRYLVEAEADFVMANDANDDN-----RRVITDN----LSKVERGTGD 141
Query: 205 ARVFMLSH-------------PKEFDVVDLDPYGSPSVFLDSAVQSVVDGGILMCTATDM 251
R ++++H FD++D+D +GS S FL A ++ GG+L T+TD
Sbjct: 142 ERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLLYLTSTDG 201
Query: 252 AVLCGGNGEVCYAKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPILSVQMDFYV 311
G A YG++ + +E+ LR+L+ A ++ P+ S ++
Sbjct: 202 YSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFSYY-SYHG 260
Query: 312 RVFVRIFTSASEMKNSPLKLSYVYQCIGCDSFHLQCLARTVTKNNSVRYLPGFGPAVPQD 371
VF + + +V C C H ++R+ P
Sbjct: 261 PVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSH------------TLRFDELGLMGCPCS 308
Query: 372 CTDCGKKFNVGGPIWSAPIHDQEWVTSILTNVRSLKERYPA----YDRISAVLTTVSE-E 426
T V GP+W P+HD +VT +L + D++ +++ S+
Sbjct: 309 DTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSIMIEESDPR 368
Query: 427 LPDVPLFLSLHNLCATLKCTSPSAVMFRSAVLNAGYRISGTHVNPLGLKSDAPM 480
LP P + + + + K SP SA++ GY S +H+ P LK+D PM
Sbjct: 369 LP--PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 420