BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000118.1_g0650.1
(337 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G30180.1 | brassinosteroid-6-oxidase 2 | Chr3:11810867-118135... 416 e-144
AT5G38970.4 | brassinosteroid-6-oxidase 1 | Chr5:15594935-155974... 399 e-139
AT5G38970.2 | brassinosteroid-6-oxidase 1 | Chr5:15594935-155974... 399 e-139
AT5G38970.1 | brassinosteroid-6-oxidase 1 | Chr5:15594935-155977... 398 e-137
AT5G38970.3 | brassinosteroid-6-oxidase 1 | Chr5:15595185-155977... 335 e-113
>AT3G30180.1 | brassinosteroid-6-oxidase 2 | Chr3:11810867-11813509
FORWARD LENGTH=465 | 201606
Length = 465
Score = 416 bits (1069), Expect = e-144, Method: Compositional matrix adjust.
Identities = 201/337 (59%), Positives = 264/337 (78%), Gaps = 5/337 (1%)
Query: 1 MLDILGKYNVVSVNGQAHKTMRGSLLSITSPSIIKNQLLPKIDQFMRSHLINWDG-KTID 59
MLDILG N+ +V+G +H+ MRGSLLS+ SP+++K+ LLPKID FMR++L WD +T+D
Sbjct: 106 MLDILGTCNIAAVHGPSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVD 165
Query: 60 IQQRSKEMVMLNAFCQVG--IKADKFEELKKEFFGLVSGVLTLPINLPGTSYRRGLQARE 117
IQ+++K M L++ Q+ +K + EE + EFF LV G L++PI++PGT+YR G+QAR
Sbjct: 166 IQEKTKHMAFLSSLLQIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARN 225
Query: 118 NIVRVLREIIEERRASPNVKYHDMLGGLMGT-DSKYDLNVEEVMDQVIMIIYSGYETVST 176
NI R+L E+++ER+ S + DMLG LM D++Y L +E+ DQV+ I+YSGYETVST
Sbjct: 226 NIDRLLTELMQERKESGET-FTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVST 284
Query: 177 TSMMTVKYLHDNPTALQELREEHLEIRKRKGPNDPIDWNDYQSMKFTRSVILETMRLATI 236
TSMM +KYLHD+P AL+ELR EHL IR+RK P++P+ +D +SMKFTR+VI ET RLATI
Sbjct: 285 TSMMALKYLHDHPKALEELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATI 344
Query: 237 VNGVLRKTMEDVEFNGFIIPKGWGIYLYTRDINYDPFLYPEPLTFNPWRWEDKSLESHKY 296
VNGVLRKT D+E NG++IPKGW IY+YTR+INYD LY +P+ FNPWRW +KSLES Y
Sbjct: 345 VNGVLRKTTHDLELNGYLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSY 404
Query: 297 YLLFGGGSRLCPGKELGIVEVSIFLHHFVTRYRFSLN 333
+LLFGGG RLCPGKELGI EVS FLH+FVT+YR+ N
Sbjct: 405 FLLFGGGVRLCPGKELGISEVSSFLHYFVTKYRWEEN 441
>AT5G38970.4 | brassinosteroid-6-oxidase 1 | Chr5:15594935-15597465
REVERSE LENGTH=384 | 201606
Length = 384
Score = 399 bits (1026), Expect = e-139, Method: Compositional matrix adjust.
Identities = 196/334 (58%), Positives = 254/334 (76%), Gaps = 5/334 (1%)
Query: 1 MLDILGKYNVVSVNGQAHKTMRGSLLSITSPSIIKNQLLPKIDQFMRSHLINWDG-KTID 59
MLDILG N+ +V+G +H+ MRGSLLS+ S +++++ +LPK+D FMRS+L W+ + ID
Sbjct: 25 MLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVID 84
Query: 60 IQQRSKEMVMLNAFCQVG--IKADKFEELKKEFFGLVSGVLTLPINLPGTSYRRGLQARE 117
IQ ++K M L++ Q+ ++ EE K FF LV G L++PI+LPGT+YR G+QAR
Sbjct: 85 IQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARN 144
Query: 118 NIVRVLREIIEERRASPNVKYHDMLGGLMGTD-SKYDLNVEEVMDQVIMIIYSGYETVST 176
NI R+LRE+++ERR S + DMLG LM + ++Y L EE+ DQV+ I+YSGYETVST
Sbjct: 145 NIDRLLRELMQERRDSGET-FTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVST 203
Query: 177 TSMMTVKYLHDNPTALQELREEHLEIRKRKGPNDPIDWNDYQSMKFTRSVILETMRLATI 236
TSMM +KYLHD+P ALQELR EHL R+RK ++P+ D +SMKFTR+VI ET RLATI
Sbjct: 204 TSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATI 263
Query: 237 VNGVLRKTMEDVEFNGFIIPKGWGIYLYTRDINYDPFLYPEPLTFNPWRWEDKSLESHKY 296
VNGVLRKT D+E NG++IPKGW IY+YTR+INYD LY +PL FNPWRW KSLES
Sbjct: 264 VNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNS 323
Query: 297 YLLFGGGSRLCPGKELGIVEVSIFLHHFVTRYRF 330
+FGGG+RLCPGKELGIVE+S FLH+FVTRYR+
Sbjct: 324 CFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRW 357
>AT5G38970.2 | brassinosteroid-6-oxidase 1 | Chr5:15594935-15597465
REVERSE LENGTH=384 | 201606
Length = 384
Score = 399 bits (1026), Expect = e-139, Method: Compositional matrix adjust.
Identities = 196/334 (58%), Positives = 254/334 (76%), Gaps = 5/334 (1%)
Query: 1 MLDILGKYNVVSVNGQAHKTMRGSLLSITSPSIIKNQLLPKIDQFMRSHLINWDG-KTID 59
MLDILG N+ +V+G +H+ MRGSLLS+ S +++++ +LPK+D FMRS+L W+ + ID
Sbjct: 25 MLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVID 84
Query: 60 IQQRSKEMVMLNAFCQVG--IKADKFEELKKEFFGLVSGVLTLPINLPGTSYRRGLQARE 117
IQ ++K M L++ Q+ ++ EE K FF LV G L++PI+LPGT+YR G+QAR
Sbjct: 85 IQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARN 144
Query: 118 NIVRVLREIIEERRASPNVKYHDMLGGLMGTD-SKYDLNVEEVMDQVIMIIYSGYETVST 176
NI R+LRE+++ERR S + DMLG LM + ++Y L EE+ DQV+ I+YSGYETVST
Sbjct: 145 NIDRLLRELMQERRDSGET-FTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVST 203
Query: 177 TSMMTVKYLHDNPTALQELREEHLEIRKRKGPNDPIDWNDYQSMKFTRSVILETMRLATI 236
TSMM +KYLHD+P ALQELR EHL R+RK ++P+ D +SMKFTR+VI ET RLATI
Sbjct: 204 TSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATI 263
Query: 237 VNGVLRKTMEDVEFNGFIIPKGWGIYLYTRDINYDPFLYPEPLTFNPWRWEDKSLESHKY 296
VNGVLRKT D+E NG++IPKGW IY+YTR+INYD LY +PL FNPWRW KSLES
Sbjct: 264 VNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNS 323
Query: 297 YLLFGGGSRLCPGKELGIVEVSIFLHHFVTRYRF 330
+FGGG+RLCPGKELGIVE+S FLH+FVTRYR+
Sbjct: 324 CFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRW 357
>AT5G38970.1 | brassinosteroid-6-oxidase 1 | Chr5:15594935-15597774
REVERSE LENGTH=465 | 201606
Length = 465
Score = 398 bits (1023), Expect = e-137, Method: Compositional matrix adjust.
Identities = 196/334 (58%), Positives = 254/334 (76%), Gaps = 5/334 (1%)
Query: 1 MLDILGKYNVVSVNGQAHKTMRGSLLSITSPSIIKNQLLPKIDQFMRSHLINWDG-KTID 59
MLDILG N+ +V+G +H+ MRGSLLS+ S +++++ +LPK+D FMRS+L W+ + ID
Sbjct: 106 MLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVID 165
Query: 60 IQQRSKEMVMLNAFCQVG--IKADKFEELKKEFFGLVSGVLTLPINLPGTSYRRGLQARE 117
IQ ++K M L++ Q+ ++ EE K FF LV G L++PI+LPGT+YR G+QAR
Sbjct: 166 IQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARN 225
Query: 118 NIVRVLREIIEERRASPNVKYHDMLGGLMGTD-SKYDLNVEEVMDQVIMIIYSGYETVST 176
NI R+LRE+++ERR S + DMLG LM + ++Y L EE+ DQV+ I+YSGYETVST
Sbjct: 226 NIDRLLRELMQERRDSGET-FTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVST 284
Query: 177 TSMMTVKYLHDNPTALQELREEHLEIRKRKGPNDPIDWNDYQSMKFTRSVILETMRLATI 236
TSMM +KYLHD+P ALQELR EHL R+RK ++P+ D +SMKFTR+VI ET RLATI
Sbjct: 285 TSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATI 344
Query: 237 VNGVLRKTMEDVEFNGFIIPKGWGIYLYTRDINYDPFLYPEPLTFNPWRWEDKSLESHKY 296
VNGVLRKT D+E NG++IPKGW IY+YTR+INYD LY +PL FNPWRW KSLES
Sbjct: 345 VNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNS 404
Query: 297 YLLFGGGSRLCPGKELGIVEVSIFLHHFVTRYRF 330
+FGGG+RLCPGKELGIVE+S FLH+FVTRYR+
Sbjct: 405 CFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRW 438
>AT5G38970.3 | brassinosteroid-6-oxidase 1 | Chr5:15595185-15597774
REVERSE LENGTH=419 | 201606
Length = 419
Score = 335 bits (859), Expect = e-113, Method: Compositional matrix adjust.
Identities = 170/309 (55%), Positives = 225/309 (72%), Gaps = 7/309 (2%)
Query: 1 MLDILGKYNVVSVNGQAHKTMRGSLLSITSPSIIKNQLLPKIDQFMRSHLINWDG-KTID 59
MLDILG N+ +V+G +H+ MRGSLLS+ S +++++ +LPK+D FMRS+L W+ + ID
Sbjct: 106 MLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVID 165
Query: 60 IQQRSKEMVMLNAFCQVG--IKADKFEELKKEFFGLVSGVLTLPINLPGTSYRRGLQARE 117
IQ ++K M L++ Q+ ++ EE K FF LV G L++PI+LPGT+YR G+QAR
Sbjct: 166 IQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARN 225
Query: 118 NIVRVLREIIEERRASPNVKYHDMLGGLMGTD-SKYDLNVEEVMDQVIMIIYSGYETVST 176
NI R+LRE+++ERR S + DMLG LM + ++Y L EE+ DQV+ I+YSGYETVST
Sbjct: 226 NIDRLLRELMQERRDSGET-FTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVST 284
Query: 177 TSMMTVKYLHDNPTALQELREEHLEIRKRKGPNDPIDWNDYQSMKFTRSVILETMRLATI 236
TSMM +KYLHD+P ALQELR EHL R+RK ++P+ D +SMKFTR+VI ET RLATI
Sbjct: 285 TSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATI 344
Query: 237 VNGVLRKTMEDVEFNGFIIPKGWGIYLYTRDINYDPFLYPEPLTFNPWRW--EDKSLESH 294
VNGVLRKT D+E NG++IPKGW IY+YTR+INYD LY +PL FNPWRW S ++H
Sbjct: 345 VNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMVSPWSHKTH 404
Query: 295 KYYLLFGGG 303
L G G
Sbjct: 405 ALCLEVGQG 413