BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000118.1_g1120.1
(420 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G02090.1 | Insulinase (Peptidase family M16) protein | Chr3:3... 476 e-165
AT3G02090.2 | Insulinase (Peptidase family M16) protein | Chr3:3... 430 e-147
AT1G51980.1 | Insulinase (Peptidase family M16) protein | Chr1:1... 102 2e-23
AT3G16480.1 | mitochondrial processing peptidase alpha subunit |... 100 1e-22
AT1G51980.2 | Insulinase (Peptidase family M16) protein | Chr1:1... 89 4e-19
>AT3G02090.1 | Insulinase (Peptidase family M16) protein |
Chr3:365624-368526 FORWARD LENGTH=531 | 201606
Length = 531
Score = 476 bits (1225), Expect = e-165, Method: Compositional matrix adjust.
Identities = 220/273 (80%), Positives = 251/273 (91%)
Query: 148 KGTEKRSVRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQLAAKEP 207
K + ++NYI THYTA RMVIAAAGAVKHE++VEQVKKLFTKLSSDPTT +QL A EP
Sbjct: 259 KSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEP 318
Query: 208 AFFTGSEVRLRDDDVPLAQFAVAFSGASWTDPDSIALMVMQSMLGSWSKSSGGGKHMGSE 267
A FTGSEVR+ DDD+PLAQFAVAF GASWTDPDS+ALMVMQ+MLGSW+K+ GGGKH+GS+
Sbjct: 319 ASFTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQTMLGSWNKNVGGGKHVGSD 378
Query: 268 LAQRVGINEIAESMMAFNTNYKDTGLFGVYAIAKPDCLDDLAYAIMYELSKLSYRVSEAD 327
L QRV INEIAES+MAFNTNYKDTGLFGVYA+AK DCLDDL+YAIMYE++KL+YRVS+AD
Sbjct: 379 LTQRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDAD 438
Query: 328 VTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANR 387
VTRARNQLKSSLLLH+DGTSP+AEDIGRQLLTYGRRIP AELFARIDAVDAST+KRVAN+
Sbjct: 439 VTRARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANK 498
Query: 388 FIFDRDVAIAAMGPIQSLPDYNWFRRRTYLLRY 420
+I+D+D+AI+A+GPIQ LPDYN FRRRTY RY
Sbjct: 499 YIYDKDIAISAIGPIQDLPDYNKFRRRTYWNRY 531
Score = 210 bits (535), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 103/157 (65%), Positives = 127/157 (80%), Gaps = 1/157 (0%)
Query: 1 MAIKHIFDLARRSRKLSSLTNRSLRQPNRFASTS-PAISESLPVSPPAPNVMIYDRIAEE 59
MA+K++ LARRS++ LT + + A S PA + +SPP P++M YD AE
Sbjct: 1 MAMKNLLSLARRSQRRLFLTQATRSSSSFSAIDSVPASASPTALSPPPPHLMPYDHAAEI 60
Query: 60 VKSKIKRLENPDSRFLQHASPHPVIIDHTNILSAPETRVTTLPNGLRVATESNLSSKTAT 119
+K+KIK+LENPD RFL++ASPHP++ H +ILSAPETRVTTLPNGLRVATESNLS+KTAT
Sbjct: 61 IKNKIKKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTAT 120
Query: 120 VGVWIDAGSRFETDETNGTAHFLEHMIFKGTEKRSVR 156
VGVWIDAGSRFE+DETNGTAHFLEHMIFKGT++R+VR
Sbjct: 121 VGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVR 157
>AT3G02090.2 | Insulinase (Peptidase family M16) protein |
Chr3:365624-368534 FORWARD LENGTH=535 | 201606
Length = 535
Score = 430 bits (1106), Expect = e-147, Method: Compositional matrix adjust.
Identities = 199/245 (81%), Positives = 227/245 (92%)
Query: 148 KGTEKRSVRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQLAAKEP 207
K + ++NYI THYTA RMVIAAAGAVKHE++VEQVKKLFTKLSSDPTT +QL A EP
Sbjct: 259 KSITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEP 318
Query: 208 AFFTGSEVRLRDDDVPLAQFAVAFSGASWTDPDSIALMVMQSMLGSWSKSSGGGKHMGSE 267
A FTGSEVR+ DDD+PLAQFAVAF GASWTDPDS+ALMVMQ+MLGSW+K+ GGGKH+GS+
Sbjct: 319 ASFTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQTMLGSWNKNVGGGKHVGSD 378
Query: 268 LAQRVGINEIAESMMAFNTNYKDTGLFGVYAIAKPDCLDDLAYAIMYELSKLSYRVSEAD 327
L QRV INEIAES+MAFNTNYKDTGLFGVYA+AK DCLDDL+YAIMYE++KL+YRVS+AD
Sbjct: 379 LTQRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDAD 438
Query: 328 VTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVANR 387
VTRARNQLKSSLLLH+DGTSP+AEDIGRQLLTYGRRIP AELFARIDAVDAST+KRVAN+
Sbjct: 439 VTRARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANK 498
Query: 388 FIFDR 392
+I+D+
Sbjct: 499 YIYDK 503
Score = 210 bits (535), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 103/157 (65%), Positives = 127/157 (80%), Gaps = 1/157 (0%)
Query: 1 MAIKHIFDLARRSRKLSSLTNRSLRQPNRFASTS-PAISESLPVSPPAPNVMIYDRIAEE 59
MA+K++ LARRS++ LT + + A S PA + +SPP P++M YD AE
Sbjct: 1 MAMKNLLSLARRSQRRLFLTQATRSSSSFSAIDSVPASASPTALSPPPPHLMPYDHAAEI 60
Query: 60 VKSKIKRLENPDSRFLQHASPHPVIIDHTNILSAPETRVTTLPNGLRVATESNLSSKTAT 119
+K+KIK+LENPD RFL++ASPHP++ H +ILSAPETRVTTLPNGLRVATESNLS+KTAT
Sbjct: 61 IKNKIKKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTAT 120
Query: 120 VGVWIDAGSRFETDETNGTAHFLEHMIFKGTEKRSVR 156
VGVWIDAGSRFE+DETNGTAHFLEHMIFKGT++R+VR
Sbjct: 121 VGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVR 157
>AT1G51980.1 | Insulinase (Peptidase family M16) protein |
Chr1:19323692-19326771 REVERSE LENGTH=503 | 201606
Length = 503
Score = 102 bits (255), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 82/263 (31%), Positives = 142/263 (53%), Gaps = 19/263 (7%)
Query: 155 VRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQLAAKEPAFFTGSE 214
+ +++ ++TA RMV+AA+G V+HE++++ + L + L P QLA K + G +
Sbjct: 245 LEEFMTENFTAARMVLAASG-VEHEELLKVAEPLTSDL---PNVPPQLAPKSQ--YVGGD 298
Query: 215 VRLRDDDVPLAQFAVAFSGASWT-DPDSIALMVMQSMLGSWSKSSGGG--KHMGSELAQR 271
R + FAVAF W + +++ V+Q ++G S GG K M S L +R
Sbjct: 299 FR-QHTGGEATHFAVAFEVPGWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRR 357
Query: 272 VGINEIAE--SMMAFNTNYKDTGLFGVYAIAKPDCLDDLAYAIMYELSKLSY-RVSEADV 328
V +NE E S AF + + DTGLFG+Y + P EL ++ +V++A +
Sbjct: 358 V-LNEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQAHL 416
Query: 329 TRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIKRVAN-- 386
RA+ KS++L++++ AEDIGRQ+LTYG R P + + +VD T+K +A+
Sbjct: 417 DRAKAATKSAVLMNLESRMIAAEDIGRQILTYGERKPVDQF---LKSVDQLTLKDIADFT 473
Query: 387 RFIFDRDVAIAAMGPIQSLPDYN 409
+ + + + + G + ++P Y+
Sbjct: 474 SKVISKPLTMGSFGDVLAVPSYD 496
Score = 66.6 bits (161), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 53/165 (32%), Positives = 87/165 (52%), Gaps = 22/165 (13%)
Query: 10 ARRSRKLSSLTNRSLRQPNRFASTSPAISESLPVSPPAPNVMIYDRIAEEVKSKIKRLEN 69
A R+R L + RSLR P R+AS+S A++E+ S P + + ++ ++ + L+
Sbjct: 6 ASRARALKGVLTRSLR-PARYASSS-AVAET---SSSTPAYLSW--LSGGSRAALTSLDM 58
Query: 70 PDSRFLQHASPHPVIIDHTNILSAPETRVTTLPNGLRVATESNLSSKTATVGVWIDAGSR 129
P LQ S P + D + + ++TTLPNGL++A+E+ + A++G+++D GS
Sbjct: 59 P----LQGVSLPPPLADK---VEPSKLQITTLPNGLKIASETT-PNPAASIGLYVDCGSI 110
Query: 130 FETDETNGTAHFLEHMIFKGTEKRSVRNYISTHYTAPRMVIAAAG 174
+E +G H LE M FK T R TH+ R + A G
Sbjct: 111 YEAPYFHGATHLLERMAFKSTLNR-------THFRLVREIEAIGG 148
>AT3G16480.1 | mitochondrial processing peptidase alpha subunit |
Chr3:5599906-5602716 FORWARD LENGTH=499 | 201606
Length = 499
Score = 100 bits (248), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 78/272 (28%), Positives = 141/272 (51%), Gaps = 15/272 (5%)
Query: 149 GTEKRSVRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQLAAKEP- 207
G + N++ +YTA RMV+AA+G V HE++++ V+ L + L + P A EP
Sbjct: 235 GLTGEVLENFVFENYTASRMVLAASG-VDHEELLKVVEPLLSDLPNVPRPA------EPK 287
Query: 208 AFFTGSEVRLRDDDVPLAQFAVAFSGASWT-DPDSIALMVMQSMLGSWSKSSGGG--KHM 264
+ + G + R + FA+AF W + ++I V+Q ++G S GG K M
Sbjct: 288 SQYVGGDFR-QHTGGEATHFALAFEVPGWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGM 346
Query: 265 GSELAQRV-GINEIAESMMAFNTNYKDTGLFGVYAIAKPDCLDDLAYAIMYELSKLS-YR 322
S L R+ ++ +S AF + + +TGLFG+Y P+ + E++ ++ +
Sbjct: 347 HSWLYLRLLNQHQQFQSCTAFTSVFNNTGLFGIYGCTSPEFASQGIELVASEMNAVADGK 406
Query: 323 VSEADVTRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGRRIPFAELFARIDAVDASTIK 382
V++ + RA+ KS++L++++ AEDIGRQ+LTYG R P + +D + I
Sbjct: 407 VNQKHLDRAKAATKSAILMNLESRMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIA 466
Query: 383 RVANRFIFDRDVAIAAMGPIQSLPDYNWFRRR 414
++ I + + +A G + ++P Y+ +R
Sbjct: 467 DFTSKVI-TKPLTMATFGDVLNVPSYDSVSKR 497
Score = 57.8 bits (138), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 45/151 (29%), Positives = 69/151 (45%), Gaps = 15/151 (9%)
Query: 82 PVIIDHTNILSAPETRVTTLPNGLRVATESNLSSKTATVGVWIDAGSRFETDETNGTAHF 141
P + DH + + + TTLPNGL +ATE + + A++G+++D GS +ET + G H
Sbjct: 63 PPLSDH---VEPSKLKTTTLPNGLTIATEMS-PNPAASIGLYVDCGSIYETPQFRGATHL 118
Query: 142 LEHMIFKGTEKRSVRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQ 201
LE M FK T RS H+ R + A G EQ+ L + +
Sbjct: 119 LERMAFKSTLNRS-------HFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVE 171
Query: 202 L---AAKEPAFFTGS-EVRLRDDDVPLAQFA 228
+ + + PAF LR V + +FA
Sbjct: 172 VLIDSVRNPAFLDWEVNEELRKVKVEIGEFA 202
>AT1G51980.2 | Insulinase (Peptidase family M16) protein |
Chr1:19323929-19326771 REVERSE LENGTH=451 | 201606
Length = 451
Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust.
Identities = 72/214 (33%), Positives = 118/214 (55%), Gaps = 14/214 (6%)
Query: 155 VRNYISTHYTAPRMVIAAAGAVKHEDIVEQVKKLFTKLSSDPTTATQLAAKEPAFFTGSE 214
+ +++ ++TA RMV+AA+G V+HE++++ + L + L P QLA K + + G +
Sbjct: 245 LEEFMTENFTAARMVLAASG-VEHEELLKVAEPLTSDL---PNVPPQLAPK--SQYVGGD 298
Query: 215 VRLRDDDVPLAQFAVAFSGASWT-DPDSIALMVMQSMLGSWSKSSGGG--KHMGSELAQR 271
R + FAVAF W + +++ V+Q ++G S GG K M S L +R
Sbjct: 299 FR-QHTGGEATHFAVAFEVPGWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWLYRR 357
Query: 272 VGINEIAE--SMMAFNTNYKDTGLFGVYAIAKPDCLDDLAYAIMYELSKLSY-RVSEADV 328
V +NE E S AF + + DTGLFG+Y + P EL ++ +V++A +
Sbjct: 358 V-LNEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQAHL 416
Query: 329 TRARNQLKSSLLLHIDGTSPVAEDIGRQLLTYGR 362
RA+ KS++L++++ AEDIGRQ+LTYG
Sbjct: 417 DRAKAATKSAVLMNLESRMIAAEDIGRQILTYGE 450
Score = 66.2 bits (160), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 52/165 (31%), Positives = 85/165 (51%), Gaps = 22/165 (13%)
Query: 10 ARRSRKLSSLTNRSLRQPNRFASTSPAISESLPVSPPAPNVMIYDRIAEEVKSKIKRLEN 69
A R+R L + RSLR P R+AS+S A++E+ +P ++ ++ + L+
Sbjct: 6 ASRARALKGVLTRSLR-PARYASSS-AVAETSSSTP-----AYLSWLSGGSRAALTSLDM 58
Query: 70 PDSRFLQHASPHPVIIDHTNILSAPETRVTTLPNGLRVATESNLSSKTATVGVWIDAGSR 129
P LQ S P + D + + ++TTLPNGL++A+E+ + A++G+++D GS
Sbjct: 59 P----LQGVSLPPPLADK---VEPSKLQITTLPNGLKIASETT-PNPAASIGLYVDCGSI 110
Query: 130 FETDETNGTAHFLEHMIFKGTEKRSVRNYISTHYTAPRMVIAAAG 174
+E +G H LE M FK T R TH+ R + A G
Sbjct: 111 YEAPYFHGATHLLERMAFKSTLNR-------THFRLVREIEAIGG 148