BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000130.1_g1510.1
(570 letters)
Database: Araport11_genes.201606.pep
48,359 sequences; 20,855,782 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G22960.1 | Pyruvate kinase family protein | Chr3:8139369-8141... 608 0.0
AT5G52920.1 | plastidic pyruvate kinase beta subunit 1 | Chr5:21... 316 e-100
AT1G32440.1 | plastidial pyruvate kinase 3 | Chr1:11712205-11714... 309 6e-98
AT3G25960.1 | Pyruvate kinase family protein | Chr3:9498439-9499... 206 1e-59
AT3G55650.1 | Pyruvate kinase family protein | Chr3:20647085-206... 199 6e-57
>AT3G22960.1 | Pyruvate kinase family protein | Chr3:8139369-8141771
FORWARD LENGTH=596 | 201606
Length = 596
Score = 608 bits (1567), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 300/522 (57%), Positives = 387/522 (74%), Gaps = 16/522 (3%)
Query: 37 NGVGSTLQVNLEEMKVTDSSSSSFPAGFLGLDVVSEGELREKGFLGMRKTKLVCTIGPAC 96
NG G+ V +E V ++ ++ +G + +D V+E EL+E GF R+TKL+CTIGPA
Sbjct: 74 NGTGA---VKSDERSVVATAVTTDTSG-IEVDTVTEAELKENGFRSTRRTKLICTIGPAT 129
Query: 97 NSLEDLERLGLGGMNIARLNMCHNTIEWHRDVIEKIKKLNEEKGFCISLMIDTEGSQMHM 156
E LE L +GGMN+ARLNMCH T +WHR VI +++LNEEKGF +++M+DTEGS++HM
Sbjct: 130 CGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAIMMDTEGSEIHM 189
Query: 157 VDLGGPSSVKAEDGSIWLFTTQKFDGFRP-FTVQASYEGFSEGITVGDELVIDGGMASFE 215
DLGG +S KAEDG +W FT + FD RP T+ SY+GF+E + VGDEL++DGGM FE
Sbjct: 190 GDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGDELLVDGGMVRFE 249
Query: 216 VIEKLGNDLRCKCIDPGLLLPRAKLSFWRDGELV-QKNIGQPTISTKDWADIDFGVSEGV 274
VIEK+G D++C C DPGLLLPRA L+FWRDG LV ++N PTIS+KDW DIDFG++EGV
Sbjct: 250 VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGV 309
Query: 275 DFIAVSFVKEANDIKTLKNYLSTRSPS-TIRVLAKIESVESLGNLEDIVEASDGIMVARG 333
DFIAVSFVK A I LK+YL+ RS I V+AKIES++SL NLE+I+ ASDG MVARG
Sbjct: 310 DFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEIILASDGAMVARG 369
Query: 334 DLGVQIPFEQIPAVQKAVVNLCRQLNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 393
DLG QIP EQ+PA Q+ +V +CR LNKPVIVASQLLESM+EYPTPTRAEVADVSE VRQ
Sbjct: 370 DLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR 429
Query: 394 ADALMLSGESAVGPNAEKALSVLRMASSRMELWTREESRHCLLSNRRLGVALSDIIAEQI 453
+DALMLSGESA+G +KAL+VLR S R+E W REE RH + + +G + SD I+E+I
Sbjct: 430 SDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIGSSFSDKISEEI 489
Query: 454 CDCAVEM----GIVFPFLHTYKCYMKHGHMASLVSRNRPNSPIFAFTDSKSTRMTLNLQW 509
C+ A +M G+ F+ Y GHMASLVSR RP+ PIFAFT + S R LNLQW
Sbjct: 490 CNSAAKMANNLGVDAVFV-----YTTSGHMASLVSRCRPDCPIFAFTTTTSVRRRLNLQW 544
Query: 510 GITPLLVELSEDIDENILKTFNLIKKNGLVKEGDVVLVIADI 551
G+ P + S+D++ N+ KTF+L+K G++K GD+V+ ++D+
Sbjct: 545 GLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDM 586
>AT5G52920.1 | plastidic pyruvate kinase beta subunit 1 |
Chr5:21463680-21466612 FORWARD LENGTH=579 | 201606
Length = 579
Score = 316 bits (810), Expect = e-100, Method: Compositional matrix adjust.
Identities = 184/490 (37%), Positives = 279/490 (56%), Gaps = 24/490 (4%)
Query: 84 RKTKLVCTIGPACNSLEDLERLGLGGMNIARLNMCHNTIEWHRDVIEKIKKLNEE-KGFC 142
RKTK+VCT+GP+ N+ E + +L GMN+AR+NM H H+ VI+ +K+ N + K
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169
Query: 143 ISLMIDTEGSQMHMVDLGGPSSVKAEDGSIWLFTTQKFDGFRPFTVQASYEGFSEGITVG 202
I++M+DT+G ++ DL P + + G + FT ++ P V +Y+ F + G
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVS-TPSCVSVNYDDFVNDVEAG 226
Query: 203 DELVIDGGMASFEVIEKLGNDLRCKCIDPGLLLPRAKLSFWRDGELVQKNIGQPTISTKD 262
D L++DGGM SF V K + ++C+ +D G L R L+ + K+ P+I+ KD
Sbjct: 227 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN------VRGKSATLPSITEKD 280
Query: 263 WADIDFGVSEGVDFIAVSFVKEANDIKTLKNYLSTRSPSTIRVLAKIESVESLGNLEDIV 322
W DI FGV VDF AVSFVK+A + LK YL S + I V+ KIES +S+ NL I+
Sbjct: 281 WEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQN-SGADIHVIVKIESADSIPNLHSII 339
Query: 323 EASDGIMVARGDLGVQIPFEQIPAVQKAVVNLCRQLNKPVIVASQLLESMVEYPTPTRAE 382
ASDG MVARGDLG ++P E++P +Q+ ++NLCR + K VIVA+ +LESM+ +PTPTRAE
Sbjct: 340 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 399
Query: 383 VADVSEVVRQYADALMLSGESAVGPNAEKALSVLRMASSRMELWTREESRHCLLSNR--- 439
V+D++ VR+ ADA+MLSGE+A G KA V+ + R E + S
Sbjct: 400 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEA--------TITSGEMPP 451
Query: 440 RLGVALSDIIAEQICDCAVEMGIVFPFLHTYKCYMKHGHMASLVSRNRPNSPIFAFTDSK 499
LG A + ++E A M + + + G MA L+S RP+ I+AFT+ K
Sbjct: 452 NLGQAFKNHMSEMFAYHATMMSNTLGT--STVVFTRTGFMAILLSHYRPSGTIYAFTNEK 509
Query: 500 STRMTLNLQWGITPLLVELSEDIDENILKTFNLIKKNGLVKEGDVVLVIADITPTCGTPS 559
+ L L G+ P+ +E ++D +E + K G+VK+G+ + ++ T
Sbjct: 510 KIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQ 569
Query: 560 AFQSIMVKTV 569
+ +I V+ V
Sbjct: 570 STHNIQVRKV 579
>AT1G32440.1 | plastidial pyruvate kinase 3 | Chr1:11712205-11714963
FORWARD LENGTH=571 | 201606
Length = 571
Score = 309 bits (792), Expect = 6e-98, Method: Compositional matrix adjust.
Identities = 196/549 (35%), Positives = 303/549 (55%), Gaps = 49/549 (8%)
Query: 22 GVSPKPCGNVMVRVGNGVGST------LQVNLEEMKVTDSS--------SSSFPAGFLGL 67
GVS P ++ G GV ST +++ +K+++ S + +F G L
Sbjct: 25 GVSLSPLRRTLI--GAGVRSTSISLRQCSLSVRSIKISEDSRKPKAYAENGAFDVGVLDS 82
Query: 68 DVVSEGELREKGFLGMRKTKLVCTIGPACNSLEDLERLGLGGMNIARLNMCHNTIEWHRD 127
+ R RKTK+VCTIGP+ +S E + +L GMN+ARLNM H H+
Sbjct: 83 SSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQI 142
Query: 128 VIEKIKKLNE---EKGFCISLMIDTEGSQMHMVDLGGPSSVKAEDGSIWLFTTQKFDGFR 184
I+ +K+ N +K I++M+DT+G ++ D+ P + E+G + FT ++ +
Sbjct: 143 TIDLVKEYNSLFVDK--AIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTIKRGVSLK 198
Query: 185 PFTVQASYEGFSEGITVGDELVIDGGMASFEVIEKLGNDLRCKCIDPGLLLPRAKLSFWR 244
TV +Y+ F + VGD L++DGGM S V K + ++C ID G L R L+
Sbjct: 199 D-TVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLN--- 254
Query: 245 DGELVQKNIGQPTISTKDWADIDFGVSEGVDFIAVSFVKEANDIKTLKNYLSTRSPSTIR 304
+ K+ P+I+ KDW DI FGV VDF AVSFVK+A + LKNYL T S + I
Sbjct: 255 ---VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCS-ADIS 310
Query: 305 VLAKIESVESLGNLEDIVEASDGIMVARGDLGVQIPFEQIPAVQKAVVNLCRQLNKPVIV 364
V+ KIES +S+ NL I+ A DG MVARGDLG ++P E++P +Q+ ++ CR ++KPVIV
Sbjct: 311 VIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIV 370
Query: 365 ASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAVGPNAEKALSVLRMASSRME 424
A+ +LESM+ +PTPTRAEV+D++ VR+ ADA+MLSGE+A G KA++V+ + R E
Sbjct: 371 ATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTE 430
Query: 425 --LWTREESRHCLLSNRRLGVAL---SDIIAEQICDCAVEMGIVFPFLHTYKCYMKHGHM 479
L R + +G + I+A + P + + + G M
Sbjct: 431 ASLPVRTSASRTTAYKGHMGQMFAFHASIMANTLSS---------PLI----VFTRTGSM 477
Query: 480 ASLVSRNRPNSPIFAFTDSKSTRMTLNLQWGITPLLVELSEDIDENILKTFNLIKKNGLV 539
A L+S RP++ IFAFT+ + L L G+ P+ +E S+D ++ ++ L++ ++
Sbjct: 478 AVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENML 537
Query: 540 KEGDVVLVI 548
KEG V ++
Sbjct: 538 KEGQHVTLV 546
>AT3G25960.1 | Pyruvate kinase family protein | Chr3:9498439-9499932
FORWARD LENGTH=497 | 201606
Length = 497
Score = 206 bits (524), Expect = 1e-59, Method: Compositional matrix adjust.
Identities = 154/483 (31%), Positives = 250/483 (51%), Gaps = 30/483 (6%)
Query: 85 KTKLVCTIGPACNSLEDLERLGLGGMNIARLNMCHNTIEWHRDVIEKIKKLNEEKGFCIS 144
KTK+VCT+GPA S+E +E+L GMN+AR N H + +H++ ++ ++ + G +
Sbjct: 17 KTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILCA 76
Query: 145 LMIDTEGSQMH--MVDLGGPSSVKAEDGSIWLFTTQKFDGFRPFTVQASYEGFSEGITVG 202
+M+DT+ + + G P +K + I + K G T+ SY+ +E + G
Sbjct: 77 VMLDTKSPVIRTGFLKEGKPIQLK-QGQEITISIDYKIQG-DSNTISMSYKKLAEDLKPG 134
Query: 203 DELVIDGGMASFEVI--EKLGNDLRCKCIDPGLLLPRAKLSFWRDGELVQKNIGQPTIST 260
D ++ G S V+ +K +RC+C + LL R ++ G +V PT++
Sbjct: 135 DVILCSDGTISLNVLSCDKYLGLVRCRCENSALLGERKNVNL--PGIVVD----LPTLTE 188
Query: 261 KDWADI-DFGVSEGVDFIAVSFVKEANDIKTLKNYLSTRSPSTIRVLAKIESVESLGNLE 319
KD DI +GV +D IA+SFV++ +D+ ++ L S S I +++K+E+ E + N +
Sbjct: 189 KDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGEHSKS-IMLMSKVENQEGVMNFD 247
Query: 320 DIVEASDGIMVARGDLGVQIPFEQIPAVQKAVVNLCRQLNKPVIVASQLLESMVEYPTPT 379
I+E SD MVARGDLG++IP E++ QK ++N KPV+ A+Q+LESM P PT
Sbjct: 248 KILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKPVVTATQMLESMTVSPRPT 307
Query: 380 RAEVADVSEVVRQYADALMLSGESAVGPNAEKALSVLRMASSRMELWTREESRHCLLSNR 439
RAE DV+ V D +MLSGE+A G + E A VL M SR+ + + +L +
Sbjct: 308 RAEATDVANAVLDGTDCVMLSGETAAGAHPETA--VLTM--SRICKEAEDFIDYDILHKK 363
Query: 440 RLG-VALSDIIAEQICDCAVEMGIVFPFLHTYKCYMKHGHMASLVSRNRPNSPIFAF--- 495
LG V+L E + AV F + G+ A LV++ RP+ PI +
Sbjct: 364 TLGMVSLPLSPIESLAASAVSTARSV-FASAIVVLTRGGYTAELVAKYRPSVPILSVIMP 422
Query: 496 -----TDSKSTRMTLNLQW-GITPLLVELSEDIDENILK-TFNLIKKNGLVKEGDVVLVI 548
+DS + L + GI P++ + D E +++ K G+ K GD ++ +
Sbjct: 423 EIAECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGFAKTKGICKTGDSIVAL 482
Query: 549 ADI 551
I
Sbjct: 483 HKI 485
>AT3G55650.1 | Pyruvate kinase family protein |
Chr3:20647085-20648617 FORWARD LENGTH=510 | 201606
Length = 510
Score = 199 bits (507), Expect = 6e-57, Method: Compositional matrix adjust.
Identities = 134/418 (32%), Positives = 221/418 (52%), Gaps = 24/418 (5%)
Query: 85 KTKLVCTIGPACNSLEDLERLGLGGMNIARLNMCHNTIEWHRDVIEKIKKLNEEKGFCIS 144
KTK++CT+GP S+E +E+L GMN+AR N H + +H++ ++ ++ + G +
Sbjct: 17 KTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILSA 76
Query: 145 LMIDTEGSQMHMVDLGGPSSVKAEDGSIWLFTTQKFDGFRPFTVQASYEGFSEGITVGDE 204
+M+DT+G ++ L ++ G + + SY+ +E + GD
Sbjct: 77 VMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISMSYKKLAEDVKPGDV 136
Query: 205 LVIDGGMASFEVI--EKLGNDLRCKCIDPGLLLPRAKLSFWRDGELVQKNIGQPTISTKD 262
++ G S V+ +K +RC+C + +L R ++ G +V PT++ KD
Sbjct: 137 ILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNL--PGIVVD----LPTLTEKD 190
Query: 263 WADI-DFGVSEGVDFIAVSFVKEANDIKTLKNYLSTRSPSTIRVLAKIESVESLGNLEDI 321
DI +GV +D IA+SFV++ +D+ ++ L S + I +++K+E+ E + N E I
Sbjct: 191 KEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSKN-IMLMSKVENQEGVMNCEKI 249
Query: 322 VEASDGIMVARGDLGVQIPFEQIPAVQKAVVNLCRQLNKPVIVASQLLESMVEYPTPTRA 381
+E SD MVARGDLG++IP E++ QK ++ + L KPV+ A+Q+LESM P PTRA
Sbjct: 250 LENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKPVVTATQMLESMTVSPRPTRA 309
Query: 382 EVADVSEVVRQYADALMLSGESAVGPNAEKALSVLRMASSRMELWTREESRHCLLSNRRL 441
E DV+ V D +MLSGE+A G + E A VL M SR+ + + +L + L
Sbjct: 310 EATDVANAVLDGTDCVMLSGETAAGAHPEAA--VLTM--SRICKEAEDFIDYDILHKKTL 365
Query: 442 GVA---LSDI--IAEQICDCAVEMGIVFPFLHTYKCYMKHGHMASLVSRNRPNSPIFA 494
G+ LS I +A + A + F K G+ A LV++ RP+ PI +
Sbjct: 366 GMVSLPLSPIESLAASVVSTAQSV-----FASAIVVLTKGGYTAELVAKYRPSVPILS 418