BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000058.1_g0040.1
(445 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010278476.1 PREDICTED: conserved oligomeric Golgi complex sub... 468 e-155
KVH88391.1 Conserved oligomeric Golgi complex subunit 5 [Cynara ... 386 e-123
XP_002279329.1 PREDICTED: conserved oligomeric Golgi complex sub... 379 e-120
>XP_010278476.1 PREDICTED: conserved oligomeric Golgi complex subunit 5 [Nelumbo
nucifera]
Length = 842
Score = 468 bits (1205), Expect = e-155, Method: Compositional matrix adjust.
Identities = 240/372 (64%), Positives = 276/372 (74%), Gaps = 45/372 (12%)
Query: 8 PSTSSSPFDFFSSHPIFSHFLSSDFHHANFANQTLSFGSAAACVEKLEEGIRLLDKQLCE 67
P +SSSP D FSS P+FS LS DF F+ Q LS GSAAA EKLEEGIRLL+KQL
Sbjct: 41 PHSSSSPLDAFSSDPVFSALLSPDFDSTRFSTQALSSGSAAARAEKLEEGIRLLEKQL-- 98
Query: 68 RQRGGLETMRWFGNNEAVWKSPMRRIGSTASATFSYNKLRSEVLSRQDDLLSLLFSIKDA 127
RSEVLSR DDLLS L S+KD
Sbjct: 99 ---------------------------------------RSEVLSRHDDLLSQLSSLKDV 119
Query: 128 ESALSVVRSGSSTLQSSVRGIRQEIADSHHQIRFKTVQLSNIHQTTELLQFTVRVLRLSK 187
ESALSVVRSG S LQSSVR +RQEI+D + QIR KTVQLSNIH T ELLQ TVRVLRLSK
Sbjct: 120 ESALSVVRSGISNLQSSVRRVRQEISDPYRQIRLKTVQLSNIHHTAELLQSTVRVLRLSK 179
Query: 188 ILRDLMAIGGSEPEKMNLSKAAQLYHEIQCLCEEKSLSGITVIDEELVWLSKTGNKIRLE 247
L+DLMA+ +EPEK++LSKAAQL+ EIQ LCEE SL+GI V+DEE++WL++TGNK+R E
Sbjct: 180 KLKDLMAVSATEPEKLDLSKAAQLHREIQILCEENSLAGIDVVDEEMIWLAETGNKLRSE 239
Query: 248 GMKVLERGMEGLNQAEVGSGLQVFYNLGELRLTVDTLINKYKNQCIKCISFALDMKAISA 307
MKVLERGMEGLNQAEVGSGLQVFYNLGELR TVD LI KYKNQ +K ++ A+D+KAISA
Sbjct: 240 AMKVLERGMEGLNQAEVGSGLQVFYNLGELRSTVDALITKYKNQGVKSVNMAMDLKAISA 299
Query: 308 SGVSFGPRGILRS----TGGGAKAKEGLWHRMHTCMNQIHSIVVAIWHLQRVLSKKRDPF 363
S +FGP G+ R+ GGGAKAKE LW RM+TCM+Q+HSI+VAIWHLQRVLSKKRDPF
Sbjct: 300 SSGNFGPGGVQRTGTPQLGGGAKAKEALWQRMNTCMDQMHSIIVAIWHLQRVLSKKRDPF 359
Query: 364 THVLLLDKVMQE 375
THVLLLD+V+Q+
Sbjct: 360 THVLLLDEVIQD 371
Score = 95.9 bits (237), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 51/94 (54%), Positives = 68/94 (72%), Gaps = 4/94 (4%)
Query: 338 CMNQIHSIVVAIWHLQ-RVLSKKRDPFTHVLLLDKVMQEEIEAVELDEHLTMLVLREIGK 396
C+ ++ +V +++ + R +D + ++L +QEEIEAV+LD HLT+LVL EIGK
Sbjct: 460 CLGRLSDLVNSVFPVSTRGSVPSKDQISRIILR---IQEEIEAVKLDGHLTLLVLHEIGK 516
Query: 397 VLHLLAERAEYQISMGPDALQVTGPATQSQLQQL 430
VLHLLAERAEYQIS GP+A QVTGPAT SQL+
Sbjct: 517 VLHLLAERAEYQISAGPEARQVTGPATPSQLKNF 550
>KVH88391.1 Conserved oligomeric Golgi complex subunit 5 [Cynara cardunculus
var. scolymus]
Length = 826
Score = 386 bits (992), Expect = e-123, Method: Compositional matrix adjust.
Identities = 216/368 (58%), Positives = 251/368 (68%), Gaps = 50/368 (13%)
Query: 15 FDFFSSHPIFSHFLSSDFHHANFANQTLSFGSAAACVEKLEEGIRLLDKQLCERQRGGLE 74
D F+S PIFS FLS DF F+++ LS G+AAA EK+++GIRLL+KQL
Sbjct: 37 LDSFASDPIFSKFLSPDFDSTRFSSEALSSGTAAARAEKIQDGIRLLEKQL--------- 87
Query: 75 TMRWFGNNEAVWKSPMRRIGSTASATFSYNKLRSEVLSRQDDLLSLLFSIKDAESALSVV 134
RSEVLSR +DLLS L S+KDA+S+L +
Sbjct: 88 --------------------------------RSEVLSRHNDLLSQLSSVKDADSSLFAI 115
Query: 135 RSGSSTLQSSVRGIRQEIADSHHQIRFKTVQLSNIHQTTELLQFTVRVLRLSKILRDLMA 194
RS STLQSSVR +R EIAD + QIR KT+QLSN+H T +LLQ TVRVLRLSK LRD+MA
Sbjct: 116 RSAVSTLQSSVRRVRSEIADPNRQIRSKTLQLSNLHFTVDLLQSTVRVLRLSKKLRDVMA 175
Query: 195 IGGSEPEKMNLSKAAQLYHEIQCLCEEKSLSGITVIDEELVWLSKTGNKIRLEGMKVLER 254
E EK++LSKAAQL+ EI LC E LSGI IDEEL W+ + G ++R EGMKVLER
Sbjct: 176 --EPETEKLDLSKAAQLHSEILRLCNENDLSGIVPIDEELKWVFEAGQRLRSEGMKVLER 233
Query: 255 GMEGLNQAEVGSGLQVFYNLGELRLTVDTLINKYKNQCIKCISFALDMKAI---SASGVS 311
G+EG NQAEVG+GLQVFYNLGELR TVD LINKYK Q +K +S ALDMK I G
Sbjct: 234 GLEGFNQAEVGAGLQVFYNLGELRATVDGLINKYKTQGVKSVSVALDMKTISSSGGGGGF 293
Query: 312 FGPRGILRST----GGGAKAKEGLWHRMHTCMNQIHSIVVAIWHLQRVLSKKRDPFTHVL 367
GP GI RS G GAKAKE LW RM TCM+Q+HS+VVAIWHLQRVLSKKRDPFTH L
Sbjct: 294 GGPGGIQRSGTPQFGSGAKAKEALWQRMATCMDQLHSVVVAIWHLQRVLSKKRDPFTHAL 353
Query: 368 LLDKVMQE 375
LLD+VMQE
Sbjct: 354 LLDEVMQE 361
Score = 66.2 bits (160), Expect = 5e-08, Method: Compositional matrix adjust.
Identities = 41/96 (42%), Positives = 59/96 (61%), Gaps = 17/96 (17%)
Query: 338 CMNQIHSIVVAIWHLQR---VLSKKRDPFTHVLLLDKVMQEEIEAVELDEHLTMLVLREI 394
C++++ +V ++ + V SK+ H+ + +QEEIEAV+LD LT+LVLREI
Sbjct: 450 CLSRLSDLVNTVFPMSNRGSVPSKE-----HISRIISRIQEEIEAVQLDARLTLLVLREI 504
Query: 395 GKVLHLLAERAEYQISMGPDALQVTGPATQSQLQQL 430
KVL LLA+RAEYQI TGPAT +Q++
Sbjct: 505 SKVLLLLAQRAEYQI---------TGPATAAQVKNF 531
>XP_002279329.1 PREDICTED: conserved oligomeric Golgi complex subunit 5 [Vitis
vinifera] CBI22100.3 unnamed protein product, partial
[Vitis vinifera]
Length = 830
Score = 379 bits (974), Expect = e-120, Method: Compositional matrix adjust.
Identities = 210/376 (55%), Positives = 262/376 (69%), Gaps = 52/376 (13%)
Query: 8 PSTSS---SPFDFFSSHPIFSHFLSSDFHHANFANQTLSFGSAAACVEKLEEGIRLLDKQ 64
P+T++ SP D F+S P FS FLS F F++ LS GSAA+ EKL++GIRLL+KQ
Sbjct: 29 PTTATGGASPLDAFASDPTFSAFLSHSFDSTRFSSAALSAGSAASTAEKLQDGIRLLEKQ 88
Query: 65 LCERQRGGLETMRWFGNNEAVWKSPMRRIGSTASATFSYNKLRSEVLSRQDDLLSLLFSI 124
L RSEVL R DLL+ L S+
Sbjct: 89 L-----------------------------------------RSEVLHRHSDLLNQLSSL 107
Query: 125 KDAESALSVVRSGSSTLQSSVRGIRQEIADSHHQIRFKTVQLSNIHQTTELLQFTVRVLR 184
KDA+SALS +R+ S+LQSSVR +R EIAD H QI+ KT+QLSN+H+TT+LLQ ++R +R
Sbjct: 108 KDADSALSTLRAAVSSLQSSVRRVRSEIADPHRQIKSKTIQLSNLHRTTDLLQHSIRAIR 167
Query: 185 LSKILRDLMAIGGSEPEKMNLSKAAQLYHEIQCLCEEKSLSGITVIDEELVWLSKTGNKI 244
LSK LRDL ++P+K++L+KAAQL+ EI LC E L+GI +I+EEL +S+ G+++
Sbjct: 168 LSKKLRDL---ASADPDKLDLAKAAQLHCEILSLCSENDLAGIDIINEELASVSEIGSRL 224
Query: 245 RLEGMKVLERGMEGLNQAEVGSGLQVFYNLGELRLTVDTLINKYKNQCIKCISFALDMKA 304
R + MKVLERGM+GLNQAEVG+GLQVFYNLGELR TVD LINKYK+QC+K +S ALDMKA
Sbjct: 225 RSDAMKVLERGMDGLNQAEVGTGLQVFYNLGELRQTVDALINKYKSQCVKSVSVALDMKA 284
Query: 305 ISASGVSFGPRGILRST-----GGGAKAKEGLWHRMHTCMNQIHSIVVAIWHLQRVLSKK 359
ISAS G +R + GGGAKAKE LW RM TCM++IHSIVVA+WHLQRVLSKK
Sbjct: 285 ISASSGGGFGPGGIRGSGTPQIGGGAKAKEALWQRMGTCMDEIHSIVVAVWHLQRVLSKK 344
Query: 360 RDPFTHVLLLDKVMQE 375
RDPFTHVLLLD+VMQE
Sbjct: 345 RDPFTHVLLLDEVMQE 360
Score = 85.5 bits (210), Expect = 4e-14, Method: Compositional matrix adjust.
Identities = 42/56 (75%), Positives = 49/56 (87%)
Query: 373 MQEEIEAVELDEHLTMLVLREIGKVLHLLAERAEYQISMGPDALQVTGPATQSQLQ 428
+QEEIEAV+LD LT+LVLREIGKVL LLA+RAEYQ+S GP+A QVTGPAT QL+
Sbjct: 482 IQEEIEAVQLDGRLTLLVLREIGKVLLLLAQRAEYQVSTGPEARQVTGPATPLQLK 537