BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000058.1_g1310.1
(492 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010654945.1 PREDICTED: putative disease resistance protein RG... 239 2e-66
CAN71072.1 hypothetical protein VITISV_000086 [Vitis vinifera] 233 4e-64
XP_010276307.1 PREDICTED: putative disease resistance protein RG... 233 8e-64
>XP_010654945.1 PREDICTED: putative disease resistance protein RGA3, partial [Vitis
vinifera]
Length = 912
Score = 239 bits (609), Expect = 2e-66, Method: Compositional matrix adjust.
Identities = 144/328 (43%), Positives = 200/328 (60%), Gaps = 16/328 (4%)
Query: 6 SRLCEVPSHLFHHLKCLRVLYMENIGL-ESLPSEIEKLIHLRYLNLSKNHQLKELPDSIS 64
S + +V +LF HL CLR L + N L E LP E+EKLIHLRYL+LS L+ELP++I
Sbjct: 567 SSVLQVSPNLFRHLTCLRALDLSNNQLIEELPKEVEKLIHLRYLDLSGCRSLRELPETIC 626
Query: 65 NLYNLQTLNLKKCHNLYQLPKGMNQLINLRYLEIKKTSNLNYLPKGIGELTNLRTLNKFV 124
+L NLQTLN+++C +L +LP+ M +LINLR+LE NL LPKGIG L++LRTL+ F+
Sbjct: 627 DLSNLQTLNIRECSSLQKLPQAMGKLINLRHLENGFVHNLKGLPKGIGRLSSLRTLDVFI 686
Query: 125 VGQSDSEGCRITELKKLNLLRGYLRIGGLGRVL-VEEALGAELLKKQHLHALGLDFEPNE 183
V ++ C+I +L+ LN LRG L I GL V EA AEL + HL L L F+ E
Sbjct: 687 VSSHGNDECQIGDLRNLNNLRGRLSIQGLDEVRDAGEAEKAELKNRVHLQHLVLKFDREE 746
Query: 184 GEITEESMEGVLQGLLPSPNLKELYVMNYPGRKFPSWTMDKLALYHLSGLELYDCMNCVE 243
G +GV + L P PNLK L + Y R++P+W M +L L L L C+ C
Sbjct: 747 G------TKGVAEALQPHPNLKSLGIYGYGDREWPNWMMGS-SLAQLKKLHLTSCVRCPC 799
Query: 244 LPELGQLRSLAVLRIYRLHKLKKIGVEFLGFGGSRGSRVAFPKLEEIFMEDMDSLEEWDL 303
LP LGQL L L I R+ +K IG EFLG S FPKL+ ++++ + L++W++
Sbjct: 800 LPPLGQLSVLEDLGILRMPSVKYIGSEFLG-----SSSTVFPKLKRLYIDSLAELKQWEI 854
Query: 304 GAFDSLKVIMPCLRQIR-ISCKNLKALP 330
+ ++IMPCL + ++C L+ LP
Sbjct: 855 KEKEE-RLIMPCLNDLTMLACPKLEGLP 881
Score = 67.4 bits (163), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 40/98 (40%), Positives = 53/98 (54%), Gaps = 9/98 (9%)
Query: 322 SCKNLKALPSFGRLPALEDLKVFSMYQVKHIGLEFYGFDSCVDDKTIAFPKLKNLHFSDM 381
SC LP G+L LEDL + M VK+IG EF G S V FPKLK L+ +
Sbjct: 793 SCVRCPCLPPLGQLSVLEDLGILRMPSVKYIGSEFLGSSSTV------FPKLKRLYIDSL 846
Query: 382 HNLENWDIGLEHDGREIVIMPCLSYLSLYNIAKLKVLP 419
L+ W+I + E +IMPCL+ L++ KL+ LP
Sbjct: 847 AELKQWEI---KEKEERLIMPCLNDLTMLACPKLEGLP 881
>CAN71072.1 hypothetical protein VITISV_000086 [Vitis vinifera]
Length = 927
Score = 233 bits (594), Expect = 4e-64, Method: Compositional matrix adjust.
Identities = 144/328 (43%), Positives = 198/328 (60%), Gaps = 16/328 (4%)
Query: 6 SRLCEVPSHLFHHLKCLRVLYME-NIGLESLPSEIEKLIHLRYLNLSKNHQLKELPDSIS 64
S + E +L HL CLR L + N +E LP E+ KLIHLRYLNLS ++L+ELP++I
Sbjct: 567 SXVLEALXNLLRHLTCLRALDLSRNRLIEELPKEVGKLIHLRYLNLSLCYRLRELPETIC 626
Query: 65 NLYNLQTLNLKKCHNLYQLPKGMNQLINLRYLEIKKTSNLNYLPKGIGELTNLRTLNKFV 124
+LYNLQTLN++ C +L +LP+ M +LINLR+LE T +L LPKGIG L++L+TL+ F+
Sbjct: 627 DLYNLQTLNIEGCSSLQKLPQAMGKLINLRHLENCNTGSLKGLPKGIGRLSSLQTLDVFI 686
Query: 125 VGQSDSEGCRITELKKLNLLRGYLRIGGLGRVL-VEEALGAELLKKQHLHALGLDFEPNE 183
V ++ C+I +L+ LN LRG L I L V EA AEL + H L L+F E
Sbjct: 687 VSSHGNDECQIGDLRNLNNLRGGLSIQRLDEVKDAGEAEKAELKNRVHFQYLTLEFGKKE 746
Query: 184 GEITEESMEGVLQGLLPSPNLKELYVMNYPGRKFPSWTMDKLALYHLSGLELYDCMNCVE 243
G +GV + L P PNLK L + NY R++P+W M +L L LE+ +C C
Sbjct: 747 G------TKGVAEALQPHPNLKSLDIFNYGDREWPNWMMGS-SLAQLKILEIGNCRRCPC 799
Query: 244 LPELGQLRSLAVLRIYRLHKLKKIGVEFLGFGGSRGSRVAFPKLEEIFMEDMDSLEEWDL 303
LP LGQL L L I+ + +K IG EFLG S FPKL+E+ + MD L++W++
Sbjct: 800 LPLLGQLPVLEKLDIWGMDGVKYIGSEFLG-----SSSTVFPKLKELNISRMDELKQWEI 854
Query: 304 GAFDSLKVIMPCLRQIRIS-CKNLKALP 330
+ + IMPCL +R C L+ LP
Sbjct: 855 KGKEE-RSIMPCLNHLRTEFCPKLEGLP 881
Score = 84.7 bits (208), Expect = 8e-14, Method: Compositional matrix adjust.
Identities = 113/387 (29%), Positives = 175/387 (45%), Gaps = 98/387 (25%)
Query: 56 LKELPDSISNLYNLQTLNLKKCHNLYQLPKGMNQLINLRYLEIKKTSNLNYLPKGIGELT 115
L+ L + + +L L+ L+L + + +LPK + +LI+LRYL + L LP+ I +L
Sbjct: 570 LEALXNLLRHLTCLRALDLSRNRLIEELPKEVGKLIHLRYLNLSLCYRLRELPETICDLY 629
Query: 116 NLRTLNKFVVGQSDSEGCRITELKKLNLLRGYLRIGGLGRVLVEEALGAELLKKQHLHAL 175
NL+TLN EGC + L+KL +A+G +L+ +HL
Sbjct: 630 NLQTLN--------IEGC--SSLQKL-----------------PQAMG-KLINLRHLENC 661
Query: 176 GLDFEPNEGEITEESMEGVLQGLLPSPNLKELYVMNYPGRKFPSWTMDKLALYHLSGLEL 235
N G S++G+ +G+ GR T+D ++ +S
Sbjct: 662 ------NTG-----SLKGLPKGI---------------GRLSSLQTLD---VFIVSSHGN 692
Query: 236 YDCMNCVELPELGQLRSLAVLR----IYRLHKLKKIG----------VEF----LGFGGS 277
+C ++G LR+L LR I RL ++K G V F L FG
Sbjct: 693 DEC-------QIGDLRNLNNLRGGLSIQRLDEVKDAGEAEKAELKNRVHFQYLTLEFGKK 745
Query: 278 RGSRVAFPKLEEIFMEDMDSLEEWDLGAFDSLKVIM-PCLRQIRI----SCKNLKALPSF 332
G++ L+ ++ SL+ ++ G + +M L Q++I +C+ LP
Sbjct: 746 EGTKGVAEALQP--HPNLKSLDIFNYGDREWPNWMMGSSLAQLKILEIGNCRRCPCLPLL 803
Query: 333 GRLPALEDLKVFSMYQVKHIGLEFYGFDSCVDDKTIAFPKLKNLHFSDMHNLENWDIGLE 392
G+LP LE L ++ M VK+IG EF G S V FPKLK L+ S M L+ W+I
Sbjct: 804 GQLPVLEKLDIWGMDGVKYIGSEFLGSSSTV------FPKLKELNISRMDELKQWEI--- 854
Query: 393 HDGREIVIMPCLSYLSLYNIAKLKVLP 419
E IMPCL++L KL+ LP
Sbjct: 855 KGKEERSIMPCLNHLRTEFCPKLEGLP 881
Score = 58.5 bits (140), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 77/292 (26%), Positives = 126/292 (43%), Gaps = 52/292 (17%)
Query: 212 YPGRKFPSWTMDKLALYHLSGLELYDCMNCVELPE-LGQLRSLAVLRIYRLHKLKKIGVE 270
Y R+ P D LY+L L + C + +LP+ +G+L +L L LK +
Sbjct: 616 YRLRELPETICD---LYNLQTLNIEGCSSLQKLPQAMGKLINLRHLENCNTGSLKGLPK- 671
Query: 271 FLGFGGSRGSRVAFPKLEEIFMEDMDSLEEWDLGAFDSLKVIMPCLRQIRISCKNLKALP 330
G G R++ + ++F+ +E +G +L NL+
Sbjct: 672 --GIG-----RLSSLQTLDVFIVSSHGNDECQIGDLRNLN--------------NLRGGL 710
Query: 331 SFGRLPALEDLKVFSMYQVK------HIGLEFYGFDSC--VDDKTIAFPKLKNLHFSDMH 382
S RL ++D ++K ++ LEF + V + P LK+L +
Sbjct: 711 SIQRLDEVKDAGEAEKAELKNRVHFQYLTLEFGKKEGTKGVAEALQPHPNLKSLDIFNYG 770
Query: 383 NLE--NWDIGLEHDGREIVIMPCLSYLSLYNIAKLKVLPSFGRLPSLESLEITIAREVER 440
+ E NW +G + L L + N + LP G+LP LE L+I V+
Sbjct: 771 DREWPNWMMGSS--------LAQLKILEIGNCRRCPCLPLLGQLPVLEKLDIWGMDGVKY 822
Query: 441 IGWEFYGNTRGTRCVTVAFPRLTNLNFSSMHKLKEWDLPEVEDQAKTIMPCL 492
IG EF G++ + FP+L LN S M +LK+W++ E+ ++IMPCL
Sbjct: 823 IGSEFLGSS------STVFPKLKELNISRMDELKQWEIKGKEE--RSIMPCL 866
>XP_010276307.1 PREDICTED: putative disease resistance protein RGA3 [Nelumbo
nucifera]
Length = 1052
Score = 233 bits (594), Expect = 8e-64, Method: Compositional matrix adjust.
Identities = 149/353 (42%), Positives = 210/353 (59%), Gaps = 18/353 (5%)
Query: 13 SHLFHHLKCLRVLYMENIGLESLPSEIEKLIHLRYLNLSKNHQLKELPDSISNLYNLQTL 72
S +FH L CLR L +EN +E LPS+IEKLIHLRYLNLS N +LKELP++I NL+NLQ+L
Sbjct: 576 SDIFHQLTCLRALNLENTHIEGLPSKIEKLIHLRYLNLSWNTELKELPETIGNLHNLQSL 635
Query: 73 NLKKCHNLYQLPKGMNQLINLRYLEIKKTSNLNYLPKGIGELTNLRTLNKFVV-GQSDSE 131
L C L +LP+G+ +L +R+LEI+ T L YLP+GIG L +L L KF++ G S
Sbjct: 636 ELNSCWKLCKLPEGIVKLTKMRHLEIEDTEELQYLPQGIGRLDSLCRLPKFIISGNSGGR 695
Query: 132 GCRITELKKLNLLRGYLRIGGLGRVLVE-EALGAELLKKQHLHALGLDFEPNEGEI---T 187
GC I ELK L L+G + I GLGR+ E EA A L KQH+H L L F+ ++G++
Sbjct: 696 GCTIKELKNLKFLQGKIEIRGLGRLKGENEANDAALKNKQHIHTLHLYFDGDDGQVVDRV 755
Query: 188 EESMEGVLQGLLPSPNLKELYVMNYPGRKFPSWTMDKLALYHLSGLELYDCMNCVEL-PE 246
EE M+ +L+GL P L+ L + + G K SW + L +L L+L C N +L P
Sbjct: 756 EEKMDSILEGLEPHQKLEGLTLNGFLGTKLASWMVRDDGLPNLRFLKLGGCKNLTKLPPA 815
Query: 247 LGQLRSLAVLRIYRLHKLKKI--GVEFLGFGGSR--GSRVAFPKLEEIFMEDMDSLEEWD 302
LG+L SL L I +H++K + G+ S G++ FP+L + + DM +LE WD
Sbjct: 816 LGKLPSLQRLSISGMHEIKYMFSGINEANASQSTDGGAKKVFPELIHLDINDMKNLEAWD 875
Query: 303 LGAFDSLK------VIMPCLRQIRI-SCKNLKALPSFGRLPALEDLKVFSMYQ 348
L ++ + +MP L + + C LK LP PA E L++ S++
Sbjct: 876 LDTEETNERQTTSITLMPRLSVLYVHHCPKLKVLPR-RIFPAAEALRILSIWH 927