BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Eca_sc000058.1_g3400.1
         (685 letters)

Database: ./nr 
           95,329,361 sequences; 35,143,497,570 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_017224490.1 PREDICTED: probable metal-nicotianamine transport...   893   0.0  
XP_010253872.1 PREDICTED: probable metal-nicotianamine transport...   889   0.0  
XP_010245475.1 PREDICTED: probable metal-nicotianamine transport...   887   0.0  

>XP_017224490.1 PREDICTED: probable metal-nicotianamine transporter YSL7 isoform X1
           [Daucus carota subsp. sativus]
          Length = 692

 Score =  893 bits (2308), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 437/685 (63%), Positives = 534/685 (77%), Gaps = 19/685 (2%)

Query: 4   DDEREEIGKMMPGPGLSIEQIFETKEVPSWGEQLTIRSLVVSVLLGFLFTFIVMKLNVTT 63
           DDE  E          S+E +FE+K+VP W +QLT+R+ VVS +LG LFTFIVMKLN+TT
Sbjct: 23  DDEFAE----------SVEMMFESKQVPPWQKQLTLRAFVVSFILGILFTFIVMKLNLTT 72

Query: 64  GIIPSMNVAAGLLGFFLVKVWTTTLSKFGIKSKPFTRQENTVIQTCVVATSGIAFSSGYG 123
           GIIPS+NV+AGLLGFF VK WT+ LSK G+  +PFTRQENTVIQTCVVATSGIAFS G+G
Sbjct: 73  GIIPSLNVSAGLLGFFFVKTWTSFLSKSGLLKQPFTRQENTVIQTCVVATSGIAFSGGFG 132

Query: 124 SYILGMTSNVASKE-EHFLSNNIKPLHILWMLGFICVVSFVGLFSIVPLRKIMILDYKLT 182
           SY+ GM+  VA +  E   + NIK   + WM+GF+ VVSF+GLFS+VPLRKIMI+D+KL 
Sbjct: 133 SYLFGMSDIVAKQSTEANAAQNIKNPQLHWMIGFLFVVSFLGLFSVVPLRKIMIIDFKLI 192

Query: 183 YPSGTATAYLINSFHTPKGARLAKQQVVELFKWFFFSFVWAFFQWFFTAADGCGFTSFPT 242
           YPSGTATA+LINSFHTP+GARLAK+QV  L K+F FSF W FFQWFFT+ D CGF  FPT
Sbjct: 193 YPSGTATAHLINSFHTPQGARLAKKQVKALGKFFSFSFAWGFFQWFFTSGDACGFKEFPT 252

Query: 243 FGLEAFTYRFYFDFSATYVGVGMICPYSINLSMLLGAFVSWGVMWPIIERQQGVWYPTSL 302
           FGLEA+  +F+FDFSATYVGVGMICPY IN+S+LLGA +SWG+MWP+IE ++G WYP  L
Sbjct: 253 FGLEAYRNKFFFDFSATYVGVGMICPYLINVSLLLGAILSWGIMWPLIEDRKGHWYPADL 312

Query: 303 PTSSVKGLQGYKVFIAIATILGDGFYHFLVVLSITTI---RMVKSKFSKKHNVSAYASSS 359
            +SS+ GLQGYKVFIAIA ILGDG Y+F+ VL  T     R +++K  +   V A   SS
Sbjct: 313 SSSSLHGLQGYKVFIAIAMILGDGLYNFVKVLGHTLFGLSRQIRTKKPETLPVEA-GDSS 371

Query: 360 AHQIPMSYDDQRRTQFFLKDQIPTSVAVGGYITLATLAIIAVPLFFHELKWYHIVIIYTI 419
           A    +SYDD+RRTQ FLKDQIPT  AV GY+T+A ++I  +P  FH LKWYH+ +IY  
Sbjct: 372 AETSSLSYDDERRTQMFLKDQIPTWFAVAGYVTVAVVSIATLPHIFHHLKWYHVAVIYIF 431

Query: 420 APILAFCNSYGCGLTDWSLASSYGKLAIFIFGAWVGVEKGGVVAGLAACGVMMSIVTVAS 479
           APILAFCN+YGCGLTDWSLAS+YGKLAIF  GAW G   GG++AGLAACGVMM+IV  AS
Sbjct: 432 APILAFCNAYGCGLTDWSLASTYGKLAIFTIGAWAGASHGGILAGLAACGVMMNIVATAS 491

Query: 480 DLMQDFRTGYLTLASPRSMFFSQIFGTLLGCILSPFVFWFFFYKAYPDLGVDGSEHPAPY 539
           DLMQDF+TGY+TLASPRSMF SQI GT +GC++SP VFW  FYKA+ +LGV GSE+PAPY
Sbjct: 492 DLMQDFKTGYMTLASPRSMFVSQIIGTAMGCVISPCVFW-LFYKAFDNLGVPGSEYPAPY 550

Query: 540 GSLYRGIALIAIEGTRALPMNCVKLAGLFFLISIFLNLLRDMLLLYQCEYHKYVPNAMAM 599
             +YR +A++ +EG  ALP NC+KL  +FF+ +I +N +RD +     +  KY+P  MAM
Sbjct: 551 ALVYRNMAILGVEGFSALPKNCLKLCYVFFIAAIVVNAIRDAV---GKKRAKYIPLPMAM 607

Query: 600 AIPFYLGGYFAIDMCVGSLIRYVWEKRDRAEADAFVPAMASGMICGDSLWGMPASILSLA 659
           AIPFYLGGYFAIDMCVGSLI +VW+K ++ +ADAF PA+ASG+ICGD +W +P+SIL+LA
Sbjct: 608 AIPFYLGGYFAIDMCVGSLILFVWQKLNKTKADAFGPAVASGLICGDGIWTLPSSILALA 667

Query: 660 KIRPPICMKFLPRDLNEKVDLLLKT 684
            I PPICMKFL R  N KVD  L +
Sbjct: 668 GINPPICMKFLSRQTNAKVDAFLGS 692


>XP_010253872.1 PREDICTED: probable metal-nicotianamine transporter YSL7 [Nelumbo
           nucifera]
          Length = 710

 Score =  889 bits (2298), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 432/675 (64%), Positives = 539/675 (79%), Gaps = 14/675 (2%)

Query: 20  SIEQIFETKEVPSWGEQLTIRSLVVSVLLGFLFTFIVMKLNVTTGIIPSMNVAAGLLGFF 79
           S E+ FE + VPSW EQLT R+ + S +L   F FIVMKL++TTGIIPS+NVAAGLLGFF
Sbjct: 38  STEKAFENEIVPSWKEQLTFRAFLASAVLSVAFCFIVMKLSLTTGIIPSLNVAAGLLGFF 97

Query: 80  LVKVWTTTLSKFGIKSKPFTRQENTVIQTCVVATSGIAFSSGYGSYILGMTSNVASK-EE 138
            ++V++  L KFG+  +PF+RQENTVIQT VVA SGIAFSSGYGSYILGM   +AS+ E+
Sbjct: 98  FIRVYSFALQKFGLLKQPFSRQENTVIQTAVVAASGIAFSSGYGSYILGMMPKIASQAED 157

Query: 139 HFLSNNIKPLHILWMLGFICVVSFVGLFSIVPLRKIMILDYKLTYPSGTATAYLINSFHT 198
               NN+KPL + WM GF+  VSFVGLFSIVPLRKIMI+DYKLTYPSGTATA+LINSFHT
Sbjct: 158 GNTVNNMKPLSLGWMYGFVFAVSFVGLFSIVPLRKIMIIDYKLTYPSGTATAFLINSFHT 217

Query: 199 PKGARLAKQQVVELFKWFFFSFVWAFFQWFFTAADGCGFTSFPTFGLEAFTYRFYFDFSA 258
            KGA+LAK+QV +LF+WF  SF+WAFFQWFFT++DGCGF+SFPTFGL+AF  R YFDFSA
Sbjct: 218 SKGAKLAKKQVNKLFEWFSMSFLWAFFQWFFTSSDGCGFSSFPTFGLQAFNKRLYFDFSA 277

Query: 259 TYVGVGMICPYSINLSMLLGAFVSWGVMWPIIERQQGVWYPTSLPTSSVKGLQGYKVFIA 318
           TYVGVGMICPY +N+S+L+GA +SW +MWP+IE+++G+WY   +  SS++G+QGY+VFI+
Sbjct: 278 TYVGVGMICPYIVNVSLLVGAILSWLIMWPLIEQKKGIWYAADISGSSLRGIQGYRVFIS 337

Query: 319 IATILGDGFYHFLVVLSITTIRMVKSKFSKKHNVSAYA-------SSSAHQIPMSYDDQR 371
           IA ILGDG +H + +L ITT   +  ++ K+ + S  A       S S H    SYDDQR
Sbjct: 338 IAMILGDGLFHVVCML-ITTGWSLMRQYKKRDSSSVVAIAGDQPSSDSTHDREESYDDQR 396

Query: 372 RTQFFLKDQIPTSVAVGGYITLATLAIIAVPLFFHELKWYHIVIIYTIAPILAFCNSYGC 431
           R   FLKDQIP  VA  GY++L+ ++I+AVP  FH++KWYHI++ Y IAPILAFCNSYGC
Sbjct: 397 RKDMFLKDQIPMWVAGLGYVSLSAISIVAVPQIFHQIKWYHILVSYIIAPILAFCNSYGC 456

Query: 432 GLTDWSLASSYGKLAIFIFGAWVGVEKGGVVAGLAACGVMMSIVTVASDLMQDFRTGYLT 491
           GLTDWSLAS+YGK+AIFI G+WVG+  GG+VAGL ACGVMMSIV+ ASDLMQDF+TGYLT
Sbjct: 457 GLTDWSLASTYGKIAIFIIGSWVGLHGGGIVAGLVACGVMMSIVSTASDLMQDFKTGYLT 516

Query: 492 LASPRSMFFSQIFGTLLGCILSPFVFWFFFYKAYPDLG-VDGSEHPAPYGSLYRGIALIA 550
           LASPR+MF SQ+ GT LGC+++P VFW F+YK+YP LG VDGS  PAPYG +YRGIA++ 
Sbjct: 517 LASPRAMFCSQVMGTALGCLIAPAVFW-FYYKSYPTLGEVDGS-FPAPYGLMYRGIAILG 574

Query: 551 IEGTRALPMNCVKLAGLFFLISIFLNLLRDMLLLYQCEY--HKYVPNAMAMAIPFYLGGY 608
           +EGT +LP +CV+L+  FF I+I LN++  +L  Y  ++  H++VP AM MAIPFYLGGY
Sbjct: 575 VEGTSSLPRHCVELSVGFFCIAIALNIVTLLLEKYDKKWRLHRFVPRAMCMAIPFYLGGY 634

Query: 609 FAIDMCVGSLIRYVWEKRDRAEADAFVPAMASGMICGDSLWGMPASILSLAKIRPPICMK 668
           FAIDMC+GSLI Y+W  +++A ADA  PA+ASGMICGDSLWG+PA+ILSLA + PP+CMK
Sbjct: 635 FAIDMCIGSLILYLWRWKNKAGADALAPAVASGMICGDSLWGIPAAILSLANVNPPLCMK 694

Query: 669 FLPRDLNEKVDLLLK 683
           FLP   N KVD  L 
Sbjct: 695 FLPASANAKVDAFLN 709


>XP_010245475.1 PREDICTED: probable metal-nicotianamine transporter YSL7 [Nelumbo
           nucifera]
          Length = 680

 Score =  887 bits (2291), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 421/686 (61%), Positives = 545/686 (79%), Gaps = 10/686 (1%)

Query: 1   MEFDDEREEIGKMMPGPGLSIEQIFETKEVPSWGEQLTIRSLVVSVLLGFLFTFIVMKLN 60
           M+   +  E   +      + E+ FE + VPSW +Q+T R+++ S +L   F FIVMKLN
Sbjct: 1   MQVAKKDVENDNVSSSSNQASEKAFENEIVPSWKDQVTFRAILASAVLSVAFCFIVMKLN 60

Query: 61  VTTGIIPSMNVAAGLLGFFLVKVWTTTLSKFGIKSKPFTRQENTVIQTCVVATSGIAFSS 120
           +TTGIIPS+NVAAGLLGFF ++V+T  L +FG+  +PFTRQENTVIQT VVA SGIAFS 
Sbjct: 61  LTTGIIPSLNVAAGLLGFFFIRVYTWVLERFGLLKQPFTRQENTVIQTAVVAASGIAFSG 120

Query: 121 GYGSYILGMTSNVASK-EEHFLSNNIKPLHILWMLGFICVVSFVGLFSIVPLRKIMILDY 179
           GYGSYILGM   VA++ ++   +NN+K L + W+ GF+  VSFVGLFSIVPLRKIMI+DY
Sbjct: 121 GYGSYILGMMPKVAAQAQDGATTNNVKHLSLGWLYGFLFAVSFVGLFSIVPLRKIMIIDY 180

Query: 180 KLTYPSGTATAYLINSFHTPKGARLAKQQVVELFKWFFFSFVWAFFQWFFTAADGCGFTS 239
           KLTYPSGTATA+LINSFHTPKGA+LAK+QV +LFKWF  SF+W+FFQWFFT  DGCGF+S
Sbjct: 181 KLTYPSGTATAFLINSFHTPKGAKLAKKQVSKLFKWFSISFLWSFFQWFFTGGDGCGFSS 240

Query: 240 FPTFGLEAFTYRFYFDFSATYVGVGMICPYSINLSMLLGAFVSWGVMWPIIERQQGVWYP 299
           FPTFGL+AF  RFYFDFSATY+GVGMICPY +N+S+L+GA +SW +MWP+IE+++G+WY 
Sbjct: 241 FPTFGLQAFQKRFYFDFSATYIGVGMICPYMVNISLLIGAILSWLIMWPLIEKKKGIWYA 300

Query: 300 TSLPTSSVKGLQGYKVFIAIATILGDGFYHFLVVLSITTIRMVKSKFSKKHNVSAYASSS 359
           + L  SS++G+QGYKVF AIA ILGDG +H +++L ITT R + +++ K+      + SS
Sbjct: 301 SDLSASSLRGIQGYKVFTAIAMILGDGLFHVVLML-ITTTRSLMNQYKKQD-----SQSS 354

Query: 360 AHQIPMSYDDQRRTQFFLKDQIPTSVAVGGYITLATLAIIAVPLFFHELKWYHIVIIYTI 419
           ++   +SYDDQRR + FLKDQIP  +A  GY+ L+ ++IIAVP  FH++KWY I++ Y I
Sbjct: 355 SNTDVISYDDQRRKEIFLKDQIPMWMAGLGYVALSVISIIAVPQVFHQIKWYQIMVAYII 414

Query: 420 APILAFCNSYGCGLTDWSLASSYGKLAIFIFGAWVGVEKGGVVAGLAACGVMMSIVTVAS 479
           APILAFCNSYGCGLTDWSLAS+YGK+AIFIFG+WV   KGG+VAGLAACGVMMSIV+ AS
Sbjct: 415 APILAFCNSYGCGLTDWSLASTYGKIAIFIFGSWVSHHKGGIVAGLAACGVMMSIVSTAS 474

Query: 480 DLMQDFRTGYLTLASPRSMFFSQIFGTLLGCILSPFVFWFFFYKAYPDLGVDGSEHPAPY 539
           DLMQDF+TGYLTLASPR+MF SQ+ GT LGC+++P VFW F+YK+YP LG  GS +PAPY
Sbjct: 475 DLMQDFKTGYLTLASPRAMFCSQVVGTALGCLIAPSVFW-FYYKSYPTLGEVGSSYPAPY 533

Query: 540 GSLYRGIALIAIEGTRALPMNCVKLAGLFFLISIFLNLLRDMLLLYQCEY--HKYVPNAM 597
             +YRGIA++ +EGT +LP +C+++A  FF  +I LNL+  +L  ++ ++  H+++P+ M
Sbjct: 534 ALMYRGIAILGVEGTSSLPKHCMEMAVGFFFFAIALNLITLLLERFENKWGLHRFIPSVM 593

Query: 598 AMAIPFYLGGYFAIDMCVGSLIRYVWEKRDRAEADAFVPAMASGMICGDSLWGMPASILS 657
            MAIPFY+GGYFAIDMC+GSLI ++WE++++A A+AF PA+ASGMICGDSLWG+PA+ILS
Sbjct: 594 CMAIPFYIGGYFAIDMCIGSLILFLWERKNKAGANAFAPAVASGMICGDSLWGIPAAILS 653

Query: 658 LAKIRPPICMKFLPRDLNEKVDLLLK 683
           LA    P+CMKFL    N KVD  L 
Sbjct: 654 LANRNSPLCMKFLSAATNAKVDAFLN 679


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