BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000058.1_g4440.1
(641 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_006379652.1 hypothetical protein POPTR_0008s08190g [Populus t... 642 0.0
EMT14774.1 Ent-kaur-16-ene synthase, chloroplastic [Aegilops tau... 623 0.0
XP_010260723.1 PREDICTED: ent-kaur-16-ene synthase, chloroplasti... 499 e-165
>XP_006379652.1 hypothetical protein POPTR_0008s08190g [Populus trichocarpa]
ERP57449.1 hypothetical protein POPTR_0008s08190g
[Populus trichocarpa]
Length = 564
Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 324/545 (59%), Positives = 387/545 (71%), Gaps = 62/545 (11%)
Query: 150 NSEGSKLYLAYTAEGWGTSDDWKSIMKYQRKNGSLFNSPSTTAAAFVHLQDTNCLNYLHS 209
N EG + YLAY +EG G DW+ MKYQRKNGSLFNSPSTTAAAF+H+QD CL+Y+ S
Sbjct: 17 NLEGRRAYLAYVSEGIGKLQDWEMAMKYQRKNGSLFNSPSTTAAAFIHIQDAECLHYIRS 76
Query: 210 LVQRFGNAVPTAYPLDISIQLHMVDTLERLGIDRHFRNEIRSVLDKMYRCWLDKDEEIFL 269
L+Q+FGNAVPT YPLDI +L MVD LERLGIDRHFR E + VLD+ YR WL +EEIF
Sbjct: 77 LLQKFGNAVPTIYPLDIYARLSMVDALERLGIDRHFRKERKFVLDETYRFWLQGEEEIFS 136
Query: 270 DISTCSSAFRILQ-----------NKRHVEHFN--------------------------- 291
D +TC+ AFRIL+ N+ +HF+
Sbjct: 137 DNATCALAFRILRLNGYDVSLDTLNQFSEDHFSNSLGGYLKDSGAALELYFPPLQVHDAL 196
Query: 292 ---------------------VDNTRVLKTSYRALNIDNRDFLELAIEDFDLCQTIHRKE 330
D+TR+LKTSYR I N+DFL+LA+EDF++CQ+I R+E
Sbjct: 197 NFSDHANLQRLAIRRRIKHYATDDTRILKTSYRCSTIGNQDFLKLAVEDFNICQSIQREE 256
Query: 331 LQQLERWVKDNRLDKLKFARQKLTYCYFSAAATLFSPELSEARMTWAKNGVLTTVVDDFF 390
+ +ERWV + RLDKLKFARQK YCYFSAAATLF+PELS+ARM+WAKNGVLTTVVDDFF
Sbjct: 257 FKHIERWVVERRLDKLKFARQKEAYCYFSAAATLFAPELSDARMSWAKNGVLTTVVDDFF 316
Query: 391 DVGGSREELVNLIELFEKWNGISTADFCSDHVEIIFRALQNTIDEIGEKAFAHQGRQVTN 450
DVGGS EELVNLIEL E+W+ +ADFCS+ VEII+ A+ +TI EIG+K+F QGR V +
Sbjct: 317 DVGGSEEELVNLIELIERWDVNGSADFCSEEVEIIYSAIHSTISEIGDKSFGWQGRDVKS 376
Query: 451 HIIEIWLSLLKSMMREAEWVLNKSVPTMDEYITNAYVSMALGPIVLPALYFVGPNLSEEA 510
+I+IWL LLKSM+ EA+W NKSVPT+DEY+T A+VS ALGPIVLPALYFVGP LSEE
Sbjct: 377 QVIKIWLDLLKSMLTEAQWSSNKSVPTLDEYMTTAHVSFALGPIVLPALYFVGPKLSEEV 436
Query: 511 IRDPEYDNLYKLMSTCGRLLNDMQGFKRERKEGKLNAVSLLMIHDNSVDTEEEATAKIRE 570
PE NLYK+ STCGRLLND + FKRE +EGKLNAVSL MIH TEEEA +
Sbjct: 437 AGHPELLNLYKVTSTCGRLLNDWRSFKRESEEGKLNAVSLYMIHSGGASTEEEAIEHFKG 496
Query: 571 IIDGCRRELLRLVL--KDSVIPRCCKDLFWKMSKVVHLFYMKNDGFTSVQEMVNAVNAVI 628
+ID RR+LL+LVL KDS+IPR CKDLFW M K++H FYMK+DGFTS EM N V A+I
Sbjct: 497 LIDSQRRQLLQLVLQEKDSIIPRPCKDLFWNMIKLLHTFYMKDDGFTS-NEMRNVVKAII 555
Query: 629 EEPIS 633
EPIS
Sbjct: 556 NEPIS 560
>EMT14774.1 Ent-kaur-16-ene synthase, chloroplastic [Aegilops tauschii]
Length = 670
Score = 623 bits (1607), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 323/646 (50%), Positives = 436/646 (67%), Gaps = 17/646 (2%)
Query: 6 ELTVSFYDTAWVAMVPSPTSPQLPCFPECVTWLLENQLSDGSWYFPHCNNLIIKDALSST 65
EL S YDTAWVAMVP P SPQ+PCFP+CV W+L+NQ S+GSW ++ I KD LSST
Sbjct: 15 ELLPSSYDTAWVAMVPLPGSPQVPCFPQCVEWILQNQQSNGSWGLIQMDSSINKDVLSST 74
Query: 66 LACIIALKKWNVGEEHVSRGLQFIRSNFSLSDDKDQHTPIGFDIIFPGMIEDATRLGLSL 125
LAC++ALK+WNVG E + RGL FI N S + D+ TPIGF+I F GM+ +GL
Sbjct: 75 LACVLALKRWNVGGELIRRGLHFIGRNLSAATDEQSATPIGFNITFSGMLSLGIEMGLEF 134
Query: 126 PLSPEAIDALLLKRDLEFKRVSESNSEGSKLYLAYTAEGWGTSDDWKSIMKYQRKNGSLF 185
P+ +D +L R++E KR+ S+G K Y+AY AEG G DW +MK+QRKNGSLF
Sbjct: 135 PVGQIDVDRILHLREVELKRLIGDKSDGRKAYMAYFAEGLGNLLDWNQVMKFQRKNGSLF 194
Query: 186 NSPSTTAAAFVHLQDTNCLNYLHSLVQRFG---NAVPTAYPL------DISIQ-LHMVDT 235
NSPSTTAAA +H D L++ + A A+ L D+S L+ VD
Sbjct: 195 NSPSTTAAALIHNFDDKALHFWLQKDEEIMLDVAACAMAFRLLRMNGYDVSSDDLYHVDE 254
Query: 236 LERLGIDRHFRNEIRSVLDKMYRCWLDKDEEIFLDISTCSSAFRILQNKRHVEHFNVDNT 295
+ + N+ +S+L+ +Y+ + E L ++ R L +KR++EHFNV +
Sbjct: 255 TTFHNSLQGYLNDTKSILE-LYKA---SEVEYALKFPFYATMER-LDHKRNIEHFNVCGS 309
Query: 296 RVLKTSYRALNIDNRDFLELAIEDFDLCQTIHRKELQQLERWVKDNRLDKLKFARQKLTY 355
++LK + N+DFL LAI+DFD Q+I++ EL LE W K+NRLD+L+FARQKL+Y
Sbjct: 310 QMLKIEQLPCCV-NQDFLALAIQDFDFSQSIYQYELLHLESWAKENRLDQLQFARQKLSY 368
Query: 356 CYFSAAATLFSPELSEARMTWAKNGVLTTVVDDFFDVGGSREELVNLIELFEKWNGISTA 415
CY +AAAT+F PELS+ARM+W+KNGVLTTVVDDFFDVGGS+EE NLI L EKW+ S
Sbjct: 369 CYLAAAATIFPPELSDARMSWSKNGVLTTVVDDFFDVGGSQEEHENLIALVEKWDEHSKD 428
Query: 416 DFCSDHVEIIFRALQNTIDEIGEKAFAHQGRQVTNHIIEIWLSLLKSMMREAEWVLNKSV 475
+F S+ V+I+F A+ T++++ A A Q R V H+IE+WL LL+SMM EAEW + + V
Sbjct: 429 EFYSEQVKILFCAIYTTVNQLAAVASAVQNRDVQKHLIELWLQLLRSMMSEAEWRMRQYV 488
Query: 476 PTMDEYITNAYVSMALGPIVLPALYFVGPNLSEEAIRDPEYDNLYKLMSTCGRLLNDMQG 535
PT+++Y++NA VS LGP VL +LYF+G +S+ + D EY+ L++LMSTCGRLLND+QG
Sbjct: 489 PTIEQYMSNAVVSFTLGPTVLTSLYFIGEKISDCVVNDQEYNELFRLMSTCGRLLNDIQG 548
Query: 536 FKRERKEGKLNAVSLLMIHDNSVDTEEEATAKIREIIDGCRRELLRLVLK-DSVIPRCCK 594
+RE EG L+ VSLL++H+ + E A IR+ I CR++LL+LVL+ D+ +PR CK
Sbjct: 549 LERESMEGNLDYVSLLVLHNGGSLSIETAKETIRKSIASCRKDLLKLVLREDTAVPRPCK 608
Query: 595 DLFWKMSKVVHLFYMKNDGFTSVQEMVNAVNAVIEEPISSPFVNSS 640
+LFWKM K VHLFY + DGF+S EMV+AVNAVI EP+ N S
Sbjct: 609 ELFWKMCKTVHLFYSQTDGFSSPNEMVSAVNAVISEPLKPQIRNPS 654
>XP_010260723.1 PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2
[Nelumbo nucifera]
Length = 742
Score = 499 bits (1286), Expect = e-165, Method: Compositional matrix adjust.
Identities = 239/353 (67%), Positives = 293/353 (83%), Gaps = 2/353 (0%)
Query: 281 LQNKRHVEHFNVDNTRVLKTSYRALNIDNRDFLELAIEDFDLCQTIHRKELQQLERWVKD 340
L+N+R++EH+N+++ R+LKTSYR LNI N +FLELAIEDF+ CQ I+ KEL++LERWV D
Sbjct: 364 LENRRNMEHYNLNHPRILKTSYRCLNIHNIEFLELAIEDFNNCQEIYHKELKELERWVND 423
Query: 341 NRLDKLKFARQKLTYCYFSAAATLFSPELSEARMTWAKNGVLTTVVDDFFDVGGSREELV 400
N+LD+LKFARQKLTYCYFSAAATLFSPELS+AR++WAKN +LTTVVDDFFD+GGS+EEL
Sbjct: 424 NKLDQLKFARQKLTYCYFSAAATLFSPELSDARISWAKNSLLTTVVDDFFDIGGSKEELE 483
Query: 401 NLIELFEKWNGISTADFCSDHVEIIFRALQNTIDEIGEKAFAHQGRQVTNHIIEIWLSLL 460
NLI+L EKW+ CS+ VEI+F A+ +T++EIG+KAF Q R VTNH++EIWL+LL
Sbjct: 484 NLIKLVEKWDENFAIGRCSEQVEIVFSAIHSTVNEIGDKAFKCQRRCVTNHLVEIWLNLL 543
Query: 461 KSMMREAEWVLNKSVPTMDEYITNAYVSMALGPIVLPALYFVGPNLSEEAIRDPEYDNLY 520
SMMREA+W+ NKSVPTMDEY+ N YVS ALGPIVLPALY VG LSEEA+R PEY NLY
Sbjct: 544 NSMMREADWLRNKSVPTMDEYMENGYVSFALGPIVLPALYLVGDTLSEEAVRSPEYHNLY 603
Query: 521 KLMSTCGRLLNDMQGFKRERKEGKLNAVSLLMIHDNSVDTEEEATAKIREIIDGCRRELL 580
KLMSTCGRLLND+QGF+RE K+GKLN VSL MIH + V EE+ ++R +I+ R+ELL
Sbjct: 604 KLMSTCGRLLNDIQGFEREGKQGKLNGVSLQMIHGSGVVKEEQVIREMRSLIENTRKELL 663
Query: 581 RLVLK--DSVIPRCCKDLFWKMSKVVHLFYMKNDGFTSVQEMVNAVNAVIEEP 631
LVL+ S++PR CKDLFWKMSKV+HLFY KNDGFTS +M+ VN VI EP
Sbjct: 664 GLVLQTNGSLVPRACKDLFWKMSKVLHLFYSKNDGFTSPHDMMRYVNEVIYEP 716
Score = 390 bits (1002), Expect = e-123, Method: Compositional matrix adjust.
Identities = 180/264 (68%), Positives = 206/264 (78%)
Query: 19 MVPSPTSPQLPCFPECVTWLLENQLSDGSWYFPHCNNLIIKDALSSTLACIIALKKWNVG 78
MVPSP SPQ P FPECV WLLENQL DGSW PH + L+IKDALSSTLAC++ALK+WN+G
Sbjct: 1 MVPSPNSPQSPYFPECVNWLLENQLPDGSWCLPHSHPLLIKDALSSTLACVLALKRWNIG 60
Query: 79 EEHVSRGLQFIRSNFSLSDDKDQHTPIGFDIIFPGMIEDATRLGLSLPLSPEAIDALLLK 138
H+ +G+ FI SNF S D+ QH PIGFDI FPGMIE A +GL LPLS DAL K
Sbjct: 61 TNHIEKGIHFIVSNFPSSTDEKQHAPIGFDITFPGMIEYAREMGLVLPLSSTIADALFRK 120
Query: 139 RDLEFKRVSESNSEGSKLYLAYTAEGWGTSDDWKSIMKYQRKNGSLFNSPSTTAAAFVHL 198
R+LEF+R SESNSEG K YLAY AEG DWK +MKYQRKNGSLFNSPSTTAAAF HL
Sbjct: 121 RNLEFRRTSESNSEGKKAYLAYIAEGLQNLQDWKEVMKYQRKNGSLFNSPSTTAAAFTHL 180
Query: 199 QDTNCLNYLHSLVQRFGNAVPTAYPLDISIQLHMVDTLERLGIDRHFRNEIRSVLDKMYR 258
QD NCL YL ++++ FG AVPT YPLDI QL M+D+L LGIDRHFRNEI++VLD+ YR
Sbjct: 181 QDANCLGYLRTVLKEFGGAVPTTYPLDIHAQLCMIDSLIGLGIDRHFRNEIKNVLDETYR 240
Query: 259 CWLDKDEEIFLDISTCSSAFRILQ 282
CWL KDE IFLD+ C+ AFRIL+
Sbjct: 241 CWLQKDERIFLDLDVCAMAFRILR 264