BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000066.1_g1010.1
(526 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_009114429.1 PREDICTED: putative ribonuclease H protein At1g65... 179 8e-48
XP_009129000.1 PREDICTED: putative ribonuclease H protein At1g65... 172 7e-45
XP_013716278.1 PREDICTED: uncharacterized protein LOC106419990 [... 168 4e-44
>XP_009114429.1 PREDICTED: putative ribonuclease H protein At1g65750 [Brassica
rapa]
Length = 384
Score = 179 bits (454), Expect = 8e-48, Method: Compositional matrix adjust.
Identities = 101/322 (31%), Positives = 159/322 (49%), Gaps = 14/322 (4%)
Query: 2 NKAANMRHVWDIISGKKTLWVEWVQKNLVKGRNFWEIKAPSDSSWCWRSILMERIDAKNM 61
NK ++ +W I ++LW WV+ N +K +FW + +SW W+++L R A+
Sbjct: 46 NKTLCLKLIWLIFCESESLWAIWVKDNYIKEASFWSLDESKVASWTWKALLKLRPLAERF 105
Query: 62 VHHIIGNGEDTKFWDDPWHPLGCLSSRFNTSFMYDSTLHRNSKVSSCIMDGNWVIPAHLR 121
+ +GNG+ FW D W LG L F S + + +SKV+ D W+ LR
Sbjct: 106 ITCTLGNGDKASFWFDSWCVLGPLERFFGPSGPMQTGMEIDSKVADACSDAGWL----LR 161
Query: 122 VMLGDIANRIEEVLID------NSEVDTVVWKCNKDGV--YSMKDTYNFLRPTKEKVGWA 173
A ++ +L + DT W+ N + YS K T+ LRP +K WA
Sbjct: 162 PARSQEAEELQILLCSVPLPSHSQSPDTYSWRVNDTDMESYSTKLTWESLRPRGQKQDWA 221
Query: 174 SIIWFKDMIPRHSFIGWLVIQQRLKTKVKLLNWGLDIDPKCCLCQNGMEGELHLFTQCSY 233
+WFK +P H+F+ W+ RL T+ +L +WGL ID CC+C + E H+F +C Y
Sbjct: 222 GKVWFKGHVPSHTFMMWVAHLDRLPTRSRLASWGLQIDTCCCVCNHFHETRDHVFLRCEY 281
Query: 234 AREVWVRITQLIGCHARVFSGWEEYIGWIINFFKGESSSNNLKKLLVIAFLYHIWGERNR 293
A ++W + +G +F W+ + WI + + L+KL V A LY +W ERN+
Sbjct: 282 AEQIWKMAIRRLGYRPVLFHTWDALLAWID--MRVRHCPSTLRKLTVQAILYRLWRERNQ 339
Query: 294 RMSKAKLLPAESVFWKIFNEVR 315
R+ A P + F +I +R
Sbjct: 340 RLHNASPTPTQVSFKEIDRLIR 361
>XP_009129000.1 PREDICTED: putative ribonuclease H protein At1g65750 [Brassica
rapa]
Length = 423
Score = 172 bits (437), Expect = 7e-45, Method: Compositional matrix adjust.
Identities = 96/341 (28%), Positives = 159/341 (46%), Gaps = 24/341 (7%)
Query: 2 NKAANMRHVWDIISGKKTLWVEWVQKNLVKGRNFWEIKAPSDSSWCWRSILMERIDAKNM 61
NK ++ +W +++ +LW +W + + +FW + SW W+ +L R A
Sbjct: 87 NKVQCLKFIWILLTSSTSLWADWHRSVHLSTSSFWTLSIDQADSWAWKRLLKLRPLAAQF 146
Query: 62 VHHIIGNGEDTKFWDDPWHPLGCLSSRFNTSFMYDSTLHRNSKVSSCIMDGNWVIP---- 117
++GNG+ T FW D W PLG L S LH+++KV+ I + W +P
Sbjct: 147 CRSLVGNGQTTSFWWDVWTPLGQLIDFIGPSGPRALRLHQDAKVADAIHNTGWTLPHPRS 206
Query: 118 -------AHLRVMLGDIANRIEEVLIDNSEVDTVVWKCNKD--GVYSMKDTYNFLRPTKE 168
AHL + + N I+ D+ +W V+S T+ +RP ++
Sbjct: 207 QREVELHAHLTTITLPLPNNID---------DSYIWVAGNSPLTVFSSAATWEVMRPRQD 257
Query: 169 KVGWASIIWFKDMIPRHSFIGWLVIQQRLKTKVKLLNWGLDIDPKCCLCQNGMEGELHLF 228
+ W ++WFK IP+H+F W RL T+ +L WG+ I P C LC +E HL
Sbjct: 258 EKDWFDVVWFKGHIPKHAFTMWTANYDRLPTRSRLAAWGMTIAPDCALCSGAVETRDHLL 317
Query: 229 TQCSYAREVWVRITQLIGCHARVFSGWEEYIGWIINFFKGESSSNNLKKLLVIAFLYHIW 288
C+++ +W + + + F W E + WI + S+ L+K+ V A +YHIW
Sbjct: 318 FTCAFSATIWREVFRRCQAPSSAFITWAELLSWIRS--SRSSAVTLLRKVAVQAVIYHIW 375
Query: 289 GERNRRMSKAKLLPAESVFWKIFNEVRLKVAGAEIKVVDNP 329
+RN + L+P +VF I EVR ++ + + P
Sbjct: 376 KQRNNLIHNRNLVPPMTVFKGIDKEVRNIISSRRHRSIFKP 416
>XP_013716278.1 PREDICTED: uncharacterized protein LOC106419990 [Brassica napus]
Length = 340
Score = 168 bits (425), Expect = 4e-44, Method: Compositional matrix adjust.
Identities = 100/324 (30%), Positives = 154/324 (47%), Gaps = 18/324 (5%)
Query: 2 NKAANMRHVWDIISGKKTLWVEWVQKNLVKGRNFWEIKAPSDSSWCWRSILMERIDAKNM 61
N+ ++ +W +IS +LWV+W Q + G++FW I A + SW W +L R A
Sbjct: 4 NQVMCLKFIWLLISKAPSLWVDWHQSTHLNGQSFWSITATASDSWAWHKLLELRPLALQF 63
Query: 62 VHHIIGNGEDTKFWDDPWHPLGCLSSRFNTSFMYDSTLHRNSKVSSCIMDGNWVIPAHLR 121
+GNG+ FW D W PLG L + + N+ V+ I NW +P
Sbjct: 64 CKSQLGNGKSASFWFDVWSPLGQLIKYIGPAGPRTLRIRENAHVADAITGSNWSLPHPRS 123
Query: 122 VMLGDIANRIE--EVLIDNSEVDTVVWKCNKDGVYSMKD--TYNFLRPTKEKVGWASIIW 177
M D+ + ++ + N D VW + T++ LRP ++ W ++W
Sbjct: 124 QMEVDLHVYLTTIDLPLPNDVDDKYVWVAGDSNFLDFRSSSTWDVLRPRQDIKTWVDVVW 183
Query: 178 FKDMIPRHSFIGWLVIQQRLKTKVKLLNWGLDIDPKCCLCQNGMEGELHLFTQCSYA--- 234
FK IP+HSF W+ R+ T+ +L+ WGL I C +C N E HL C Y+
Sbjct: 184 FKGAIPKHSFNMWIANYDRMPTRSRLVAWGLPISAACPICSNFDETRDHLLLTCDYSLVI 243
Query: 235 -REVWVRITQLIGCHARVFSGWEEYIGWIINFFKGESSS--NNLKKLLVIAFLYHIWGER 291
REV+ R Q IG F+ W E + WI + +S + L+K+ +YHIW +R
Sbjct: 244 WREVFSRCQQPIGA----FTNWSELLSWI----RAATSRRLSLLRKIATQGVIYHIWKQR 295
Query: 292 NRRMSKAKLLPAESVFWKIFNEVR 315
N + +L+P +VF I E+R
Sbjct: 296 NNMVHNQQLIPPAAVFIIIDKEIR 319