BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000071.1_g0290.1
(738 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010257110.1 PREDICTED: cucumisin-like [Nelumbo nucifera] 888 0.0
XP_010273983.1 PREDICTED: cucumisin-like [Nelumbo nucifera] 823 0.0
XP_011032225.1 PREDICTED: cucumisin-like [Populus euphratica] 801 0.0
>XP_010257110.1 PREDICTED: cucumisin-like [Nelumbo nucifera]
Length = 731
Score = 888 bits (2295), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 440/740 (59%), Positives = 565/740 (76%), Gaps = 15/740 (2%)
Query: 1 MAIYNLNLSLLLLKIFFILSLVGVNIIGSGGSMAEDKKIYIVYMGGLPSETEYSPTSHHE 60
MAI +LS + + F L+++ + G++ E++K++IVYMG LP E++YS TSHH
Sbjct: 1 MAILR-SLSFSHISVIF-LAIISIQC----GAIDEERKVHIVYMGALP-ESQYSLTSHHI 53
Query: 61 SILQEVLDDSLVKDVLIHSYKRSFNGFAAKLTEREIQKLHGREDFVSMFPARNIELHTTR 120
+IL+EV+++S + L+HSYKRSFNGFAAKL +RE QKL +E VS+FP+R+++L TTR
Sbjct: 54 NILEEVVEESNATESLVHSYKRSFNGFAAKLNDRERQKLARKEGVVSVFPSRSLQLQTTR 113
Query: 121 SWDFLGFSETVERVLAAESDIIVGVLDSGIWPESESFNDDGFGPPPNKWKGVCKGGKNFT 180
SWDF+G +ETV+RV E D+I+GV+DSGIWPESESF+D+GFGPPP KWKG C GGKNFT
Sbjct: 114 SWDFMGLTETVKRVPEVEGDVIIGVIDSGIWPESESFSDEGFGPPPKKWKGACNGGKNFT 173
Query: 181 CNNKLIGARYYSE---EDSARDTVGHGTHTASTAAGNKVNDVSFFDIAKGNARGAVPSAR 237
CNNKLIGAR Y++ +++ARDTVGHG+HTASTAAGNKV D F+ +A+GNARG VPSAR
Sbjct: 174 CNNKLIGARTYTDLGPDNTARDTVGHGSHTASTAAGNKVKDAGFYGLAQGNARGGVPSAR 233
Query: 238 IAVYKVCGESGCSDADILAGFDDAIADGVDILSVSLGALVHRDLDQDPIAIGAFHAMQNG 297
IA YKVC E GCSDADILA FDDAIADGVDI+S+SLG+ + D+ QD IAIG+FHAMQNG
Sbjct: 234 IAAYKVCAEGGCSDADILAAFDDAIADGVDIISISLGSSLAIDMVQDSIAIGSFHAMQNG 293
Query: 298 ILTSNSAGNSGNGILFFRTTSNAPWLFTVGATTTDRKIINKIVLGNGKK--LEGRAVNSF 355
ILTS+SAGNSG G+ T S APWL +V A++TDR+II+K+VLG+G L G +VNSF
Sbjct: 294 ILTSHSAGNSGPGL--GTTASVAPWLLSVAASSTDRRIIDKVVLGDGTTTTLVGTSVNSF 351
Query: 356 ELNGKRFPILYGENVSKNCSIELARACSDNCLDKDLVKDKIVICERRPRWEEALEKNVLG 415
+L G++F ++YG+N S CS + R C + CLD +LVK K+V+C+ + EE + LG
Sbjct: 352 DLKGEKFDLIYGKNASLRCSDDDVRLCKNGCLDANLVKGKVVVCDEVSKGEEPVHARALG 411
Query: 416 TILIFDSEDSDVASIYPLPATHLNTTVGKYVKAYFNSTKIPLGTILKSESVKDSEAPLVA 475
TI+I D+ +D IYPLPAT L G+ VK+Y NST+ P ILKSE++ DS AP+VA
Sbjct: 412 TIMI-DNRFNDFGRIYPLPATLLIPENGEKVKSYMNSTRNPQANILKSEAIHDSIAPVVA 470
Query: 476 AFSSPGPNDILPDIIKPDISAPGIDILAAFSPKANPSRVSGDKRSVKYSVLSGTSMSCPH 535
+FSS GPN I+ +IIKPDISAPG+DILAAFSP A+PS + DKRSVKYS++SGTSM+CPH
Sbjct: 471 SFSSRGPNVIIAEIIKPDISAPGVDILAAFSPLASPSGIKSDKRSVKYSIMSGTSMACPH 530
Query: 536 ATGAAAYVKSFHPDWSPSAIKSALMTTAFPMNGNKNKGVFDFGAGNIDPVKAVNPGLVYD 595
TGAAAYVKSFHP+WSPSAIKSALMTTA+PMN KN+ F +GAG+IDPVKAVNPGLVY+
Sbjct: 531 VTGAAAYVKSFHPEWSPSAIKSALMTTAWPMNATKNELEFAYGAGHIDPVKAVNPGLVYE 590
Query: 596 ILTNDYVTMLCDIGYNSSKVKLITNHTCSENRVANRTARDLNYPSFGAKVQDGESIKIKF 655
L +DY+ MLC+I Y+S +++ IT S + +A+DLNYPS G V ++ K +
Sbjct: 591 TLKSDYIQMLCNINYDSWRIREITGERISCPEASQGSAKDLNYPSMGVMVTGEKAFKSNW 650
Query: 656 PRTVTNVGRENSTYKAKITSDDRIKVVVEPSVLSFESVNEKKSFVLNVEVDTLQKDETAT 715
RTVTNVG NS YKA +TSD ++++ V P VLSF+++NE K+FV+ V L + +
Sbjct: 651 TRTVTNVGSANSIYKATVTSDPKLQITVNPDVLSFQALNENKTFVVTVAGGELPDNTVYS 710
Query: 716 AILHWSDGIHSVRSPIVVYT 735
A L WSDG HSVRSPIVVYT
Sbjct: 711 ASLVWSDGTHSVRSPIVVYT 730
>XP_010273983.1 PREDICTED: cucumisin-like [Nelumbo nucifera]
Length = 743
Score = 823 bits (2126), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 419/742 (56%), Positives = 537/742 (72%), Gaps = 19/742 (2%)
Query: 3 IYNLNLSLLLLKIFFILSLVGVNIIGSGGSMAEDKKIYIVYMGGLPSETEYS--PTSHHE 60
+ +L+ S +L+ ++S+ G+ ED+K++IVYMG L ++ + +S H
Sbjct: 4 LRSLSFSHILVVFMAVISI-------PCGATEEDRKVHIVYMGALNTDQDDGXLTSSQHL 56
Query: 61 SILQEVLDD-SLVKDVLIHSYKRSFNGFAAKLTEREIQKLHGREDFVSMFPARNIELHTT 119
SIL++V++ +L L+ SYKRSFNGFAAKL +RE QKL + +S+FP+R ++LHTT
Sbjct: 57 SILRQVVEGRNLATGSLVRSYKRSFNGFAAKLNKREKQKLESMDGVLSVFPSRTLQLHTT 116
Query: 120 RSWDFLGFSETVERVLAAESDIIVGVLDSGIWPESESFNDDGFGPPPNKWKGVCKGGKNF 179
RSW+F+G + +R AESD+IVGVLD GIWPESESF+D+GFGPPP KWKG C GG NF
Sbjct: 117 RSWNFMGLTAKTKRQPNAESDVIVGVLDXGIWPESESFSDEGFGPPPKKWKGTCNGGSNF 176
Query: 180 TCNNKLIGARYYSEED-SARDTVGHGTHTASTAAGNKVNDVSFFDIAKGNARGAVPSARI 238
TCNNKLIGAR YS D +ARD GHGTHTASTAAGN+V +VSF+ +A+GNARG VPSARI
Sbjct: 177 TCNNKLIGARVYSLNDETARDKEGHGTHTASTAAGNRVVNVSFYGLARGNARGGVPSARI 236
Query: 239 AVYKVCGESGCSDADILAGFDDAIADGVDILSVSLGALVHRDLDQDPIAIGAFHAMQNGI 298
AVYK+C GC + DIL+ FDDAIADGVDI+S+S+G D D IAIGAFHAM GI
Sbjct: 237 AVYKICNSVGCREEDILSAFDDAIADGVDIISISVGGAQAFDFSSDSIAIGAFHAMAKGI 296
Query: 299 LTSNSAGNSGNGILFFRTTSNAPWLFTVGATTTDRKIINKIVLGNGKKLEGRAVNSFELN 358
LTSNSAGNSG +S APW+F+V A++TDR+II+K+VLG+G KL G++VNSF
Sbjct: 297 LTSNSAGNSGP--FAASVSSVAPWMFSVAASSTDRQIIDKVVLGDGTKLVGKSVNSFGAR 354
Query: 359 GKRFPILYGENVSKNCSIELARACSDNCLDKDLVKDKIVICERRPRWEEALEKNVLGTIL 418
++ ++YG + S C + + C+ +CLD DLVK KIV+C++ EE + LGTI+
Sbjct: 355 NRKVDLVYGRSASTKCDPDSVQLCASDCLDGDLVKGKIVVCDKISIGEEPMRVGALGTIM 414
Query: 419 IFDSEDSDVASIYPLPATHLNTTVGKYVKAYFNSTKIPLGTILKSESVKDSEAPLVAAFS 478
I D+E +D + IYPLPA L G+ +K Y NST+ P IL+SE++ DS APLV +FS
Sbjct: 415 I-DNELNDFSLIYPLPAVLLTPENGENLKLYMNSTRDPQANILRSEAIHDSSAPLVVSFS 473
Query: 479 SPGPNDILPDIIKPDISAPGIDILAAFSPKANPSRVSGDKRSVKYSVLSGTSMSCPHATG 538
S GPN I PDIIKPD++APG+DIL+AFSP A+ S + DKRSVKYS+LSGTSMSCPH TG
Sbjct: 474 SRGPNLITPDIIKPDVTAPGVDILSAFSPIASVSDSNADKRSVKYSILSGTSMSCPHVTG 533
Query: 539 AAAYVKSFHPDWSPSAIKSALMTTAFPMNGNKNKGV-FDFGAGNIDPVKAVNPGLVYDIL 597
+AAYVKSFHPDWSP+AIKSALMTTA PM+ +N+ F +GAG+IDPVKA NPGLVYD
Sbjct: 534 SAAYVKSFHPDWSPAAIKSALMTTARPMSSAQNEDAEFAYGAGHIDPVKARNPGLVYDAQ 593
Query: 598 TNDYVTMLCDIGYNSSKVKLIT---NHTCSENRVANRTARDLNYPSFGAKVQDGESIKIK 654
DY+ MLC+IGY+S +V+LI+ + +C + +A+DLNYPS G + ++
Sbjct: 594 KGDYIQMLCNIGYDSKRVRLISGDNSSSCPKQANGKGSAKDLNYPSMGLYIDGFKAFNSN 653
Query: 655 FPRTVTNVGRENSTYKAKITSDDRIKVVVEPSVLSFESVNEKKSFVLNVEVDTLQ-KDET 713
F RTVTNVG NSTYKAK+TSD +K+ V+PSVLSF+++NEKKSFV V L T
Sbjct: 654 FTRTVTNVGFPNSTYKAKVTSDSTMKISVKPSVLSFKALNEKKSFVATVGGSALSLTHST 713
Query: 714 ATAILHWSDGIHSVRSPIVVYT 735
A L WSDGIHSVRSPIVVYT
Sbjct: 714 ARGSLVWSDGIHSVRSPIVVYT 735
>XP_011032225.1 PREDICTED: cucumisin-like [Populus euphratica]
Length = 710
Score = 801 bits (2068), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 402/701 (57%), Positives = 504/701 (71%), Gaps = 10/701 (1%)
Query: 37 KKIYIVYMGGLPSETEYSPTSHHESILQEVLDDSLVKDVLIHSYKRSFNGFAAKLTEREI 96
K+ YIVYMG LP E EYSP+SHH S+LQEV+ DS ++VL+ SYKRSFNGF+AKLT E
Sbjct: 4 KQEYIVYMGSLP-EGEYSPSSHHLSLLQEVVKDSSSENVLVRSYKRSFNGFSAKLTSEEA 62
Query: 97 QKLHGREDFVSMFPARNIELHTTRSWDFLGFSETVERVLAAESDIIVGVLDSGIWPESES 156
QKL +++ VS+FP+ ++L TTRSWDF+GF+ T SD+IVGV+D+GIWPESES
Sbjct: 63 QKLASKKEVVSIFPSTTLQLQTTRSWDFMGFNVTASGKRGTHSDLIVGVIDTGIWPESES 122
Query: 157 FNDDGFGPPPNKWKGVCKGGKNFTCNNKLIGARYYSEEDSARDTVGHGTHTASTAAGNKV 216
FNDDGFGPPP KW+GVC+GG+NFTCNNK+IGAR+YS SARD +GHG+HTASTAAGN V
Sbjct: 123 FNDDGFGPPPRKWRGVCEGGENFTCNNKIIGARHYSFS-SARDDLGHGSHTASTAAGNIV 181
Query: 217 NDVSFFDIAKGNARGAVPSARIAVYKVCGESGCSDADILAGFDDAIADGVDILSVSLGAL 276
SF+ +A+G ARG VPSARI+ YKVCG C +DIL+ FDDAIADGVDI+++S+GA
Sbjct: 182 KKASFYGLAQGTARGGVPSARISAYKVCGPGSCQSSDILSAFDDAIADGVDIITISIGAN 241
Query: 277 VHRDLDQDPIAIGAFHAMQNGILTSNSAGNSGNGILFFRTTSNAPWLFTVGATTTDRKII 336
++ D D IAIG FH+M GILT SAGN NG + S APW+FTV A++TDR+II
Sbjct: 242 QAQEFDTDVIAIGGFHSMAKGILTLQSAGN--NGPVLGSVASVAPWIFTVAASSTDRRII 299
Query: 337 NKIVLGNGKKLEGRAVNSFELNGKRFPILYGENVSKNCSIELARACSDNCLDKDLVKDKI 396
+K++LGNGK L G +VNSF L GK+FP++YG+ S+ C A C CLD+ LVK KI
Sbjct: 300 DKVILGNGKTLVGNSVNSFSLKGKKFPLVYGKVASRECKHLEASLCYSGCLDRALVKGKI 359
Query: 397 VICERRPRWEEALEKNVLGTILIFDSEDSDVASIYPLPATHLNTTVGKYVKAYFNSTKIP 456
V+C+ EA LG IL E D++ I PLP L VK+Y NSTK P
Sbjct: 360 VVCDDVNGRTEAKRAGALGAILPISFE--DISFILPLPGLSLTEDKLNAVKSYLNSTKKP 417
Query: 457 LGTILKSESVKDSEAPLVAAFSSPGPNDILPDIIKPDISAPGIDILAAFSPKANPSRVSG 516
ILKSE++KD+ AP VA+FSS GPN I+ DI+KPD SAPG DILAAF P +P+ +
Sbjct: 418 SANILKSEAIKDNAAPEVASFSSRGPNPIISDILKPDASAPGADILAAFPPVLSPTDDTA 477
Query: 517 DKRSVKYSVLSGTSMSCPHATGAAAYVKSFHPDWSPSAIKSALMTTAFPMN-GNKNKGVF 575
DKR VKYSV+SGTSM+CPHA G AAYVK+ HPDWS SAIKSA+MTTA+PMN ++ G F
Sbjct: 478 DKRHVKYSVMSGTSMACPHAAGVAAYVKAAHPDWSASAIKSAIMTTAWPMNVTERSDGEF 537
Query: 576 DFGAGNIDPVKAVNPGLVYDILTNDYVTMLCDIGYNSSKVKLIT--NHTCSENRVANRTA 633
FG+G+I+P A++PGLVY+ +DY+ + C +GY + K++ I+ N +CS+ N
Sbjct: 538 AFGSGHINPATAIHPGLVYETQKSDYIQLFCGLGYTAEKIRQISGDNSSCSK-AARNTLP 596
Query: 634 RDLNYPSFGAKVQDGESIKIKFPRTVTNVGRENSTYKAKITSDDRIKVVVEPSVLSFESV 693
RDLNYPS AKV ES +IKF RTVTNVGR NSTYKAKI S +K+ V P LSF+S+
Sbjct: 597 RDLNYPSMAAKVAVEESFRIKFHRTVTNVGRANSTYKAKIFSRSSLKIKVVPEALSFKSL 656
Query: 694 NEKKSFVLNVEVDTLQKDETATAILHWSDGIHSVRSPIVVY 734
EKKSF + + L + +A L WSDG HSVR+PIVVY
Sbjct: 657 KEKKSFAVTIVGRDLTHNSILSASLVWSDGSHSVRNPIVVY 697