BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000076.1_g1150.1
(343 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010259183.1 PREDICTED: polygalacturonase-like [Nelumbo nucifera] 380 e-128
XP_010251524.1 PREDICTED: polygalacturonase-like [Nelumbo nucifera] 335 e-110
OAY50275.1 hypothetical protein MANES_05G122500 [Manihot esculenta] 334 e-110
>XP_010259183.1 PREDICTED: polygalacturonase-like [Nelumbo nucifera]
Length = 391
Score = 380 bits (975), Expect = e-128, Method: Compositional matrix adjust.
Identities = 202/396 (51%), Positives = 270/396 (68%), Gaps = 60/396 (15%)
Query: 1 MATIIYSFLFSLLFIFPFLPSSSTAYLLINVADFGATAGDGNTDSSPSFIRAWIKACTSN 60
MA ++ S + S+ FI P L +S A INVADFGA A DG TD+S +F+ AW AC+S
Sbjct: 1 MARVMNSLMSSVFFI-PLLLTSIDAD--INVADFGAKA-DGKTDASEAFLAAWAAACSST 56
Query: 61 LPTTIYIPPKRYFVRPVIFQGPCTSSNITFHLDGILVAPDDYVDMGSNENWFTFTGVNGL 120
PTTIY+P K+YF+ PV+F+GPC +S IT +D ILVAPD Y +M S+ENW F V+GL
Sbjct: 57 KPTTIYLPAKKYFLGPVVFRGPCNNSRITLQMDAILVAPD-YQEMASSENWIMFDVVDGL 115
Query: 121 SIVGGGYLDGQGARLWSCKGTSSRKCPPGATSLAVYGSKDVLIRNITLIDAKLYHIVIHS 180
S++GG YLDG+G+ W CKG S+ C G+TSLA+Y SK+++++N+T I++KL HI++H+
Sbjct: 116 SVIGG-YLDGRGSSFWKCKGVSN-NCSAGSTSLAIYSSKNIVVQNLTSINSKLGHILVHT 173
Query: 181 CKNVKIEGVRIYAPENSPNTDGIHIQNAVNVRVLK----------TVFTGTT-------- 222
C++V ++GVRI AP+ SP+TDGIH+QN++NV +L ++ GTT
Sbjct: 174 CRDVVLQGVRIRAPDQSPHTDGIHVQNSINVSILNAGIKTGDDCISIGQGTTNLWIERVA 233
Query: 223 -----------------------------------NGLRIKSWGRPSNGFVKGVVFKQVV 247
NG+RIKSWGRPSNG+VKGVVFK+V+
Sbjct: 234 CGPGHGISIGSLAKDLEEEGVENVVVQSVVFTGTQNGVRIKSWGRPSNGYVKGVVFKKVL 293
Query: 248 MKNVENPIVIDQNYCPWYEGCPDQNSGIKISHVTYSNIKGTSASQVAMKFDCSATSPCRG 307
MKNV NPIVIDQNYCP E CP Q+SG++++ V+Y +I+GTSA+QVA+ FDCS TSPC+
Sbjct: 294 MKNVNNPIVIDQNYCPRNENCPYQDSGVEVNGVSYIDIEGTSATQVALNFDCSPTSPCKE 353
Query: 308 IGLKDIQLTYQRQPAQSFCRNIHGIARGVITPPSCL 343
I L+DI LTYQ +PAQS C+N G+ARG I PPSCL
Sbjct: 354 ISLQDINLTYQNKPAQSSCQNAFGVARGQIVPPSCL 389
>XP_010251524.1 PREDICTED: polygalacturonase-like [Nelumbo nucifera]
Length = 384
Score = 335 bits (859), Expect = e-110, Method: Compositional matrix adjust.
Identities = 172/367 (46%), Positives = 225/367 (61%), Gaps = 56/367 (15%)
Query: 30 NVADFGATAGDGNTDSSPSFIRAWIKACTSNLPTTIYIPPKRYFVRPVIFQGPCTSSNIT 89
NV +GA DG TDS+ +F+ AW AC S IY+PP RY + +F G C S+ IT
Sbjct: 21 NVVSYGAKP-DGTTDSTSAFLNAWTGACGSASSAMIYVPPGRYLLNHAVFSGACRSTGIT 79
Query: 90 FHLDGILVAPDDYVDMGSNENWFTFTGVNGLSIVGGGYLDGQGARLWSCKGTSSRKCPPG 149
+DG LVAP DY +GS+ W TF GVNG+SI GG LDGQG+ LW+CK + + CP G
Sbjct: 80 IRIDGTLVAPSDYWALGSSGYWLTFQGVNGVSI-SGGTLDGQGSSLWACK-AAGKSCPVG 137
Query: 150 ATSLAVYGSKDVLIRNITLIDAKLYHIVIHSCKNVKIEGVRIYAPENSPNTDGIHIQNAV 209
A SL+ S++++IR ++ I++KL+HIVI C+NV ++GV I A NSPNTDGIH++ +
Sbjct: 138 ARSLSFVNSRNIVIRGLSSINSKLFHIVIDGCQNVYLQGVTIMASGNSPNTDGIHVELST 197
Query: 210 NVRVLKT----------------------------------------------------- 216
V +L++
Sbjct: 198 GVTILRSNIMTGDDCISIGPGTTNLWIENIVCGPGHGISIGSLGRSLNEPGVQNVTVKTV 257
Query: 217 VFTGTTNGLRIKSWGRPSNGFVKGVVFKQVVMKNVENPIVIDQNYCPWYEGCPDQNSGIK 276
F GT NGLRIKSWGRPSNGFVKGV+F+ M+NV+NPI+IDQNYCP GCPDQ SG+K
Sbjct: 258 TFNGTQNGLRIKSWGRPSNGFVKGVLFQNATMENVQNPIIIDQNYCPHNVGCPDQASGVK 317
Query: 277 ISHVTYSNIKGTSASQVAMKFDCSATSPCRGIGLKDIQLTYQRQPAQSFCRNIHGIARGV 336
IS VTY +I+GTSA+QVA+K DCS+ PC I L+DI+L+ + Q +S C N GIARG+
Sbjct: 318 ISKVTYKHIQGTSATQVAVKLDCSSKHPCTDITLEDIKLSCKNQNVESSCANAGGIARGL 377
Query: 337 ITPPSCL 343
+ P SCL
Sbjct: 378 VQPSSCL 384
>OAY50275.1 hypothetical protein MANES_05G122500 [Manihot esculenta]
Length = 392
Score = 334 bits (857), Expect = e-110, Method: Compositional matrix adjust.
Identities = 176/396 (44%), Positives = 237/396 (59%), Gaps = 57/396 (14%)
Query: 1 MATIIYSFLFSLLFIFPFLPSSSTAYLLINVADFGATAGDGNTDSSPSFIRAWIKACTSN 60
MA S LF +FI F S + A NV ++GA DG TDS+ +F+ AW +AC S
Sbjct: 1 MAKHETSHLFVAIFIILFASSLANAARY-NVLNYGAKP-DGRTDSTKAFLAAWTQACGSV 58
Query: 61 LPTTIYIPPKRYFVRPVIFQGPCTSSNITFHLDGILVAPDDYVDMGSNENWFTFTGVNGL 120
P T+Y+P R+F+R V+FQGPC ++ I FH+ G L+AP DY +G+ NW +F VNG+
Sbjct: 59 KPATVYVPAGRFFLRNVVFQGPCKNNAILFHIGGTLLAPSDYRVIGNAGNWLSFQYVNGV 118
Query: 121 SIVGGGYLDGQGARLWSCKGTSSRKCPPGATSLAVYGSKDVLIRNITLIDAKLYHIVIHS 180
+V GG LDGQG LW+CK S + CP GATSL S ++ I +T ++++++HIVI+
Sbjct: 119 -MVSGGVLDGQGPALWACK-ASGKDCPSGATSLGFSNSNNIAISGLTSLNSQMFHIVING 176
Query: 181 CKNVKIEGVRIYAPENSPNTDGIHIQNAVNVRVLKT------------------------ 216
C NVK++GV + A +SPNTDGIH+Q + V +L +
Sbjct: 177 CNNVKVQGVTVSASGHSPNTDGIHVQLSSGVTILNSKIRTGDDCISIGAGTTNLWIEKVA 236
Query: 217 -----------------------------VFTGTTNGLRIKSWGRPSNGFVKGVVFKQVV 247
FT T NGLRIKSWGRPS+GFV+ V+F+
Sbjct: 237 CGPGHGISIGSLGKDLKEPGVQNVTVKSVTFTDTQNGLRIKSWGRPSSGFVRNVLFQHAT 296
Query: 248 MKNVENPIVIDQNYCPWYEGCPDQNSGIKISHVTYSNIKGTSASQVAMKFDCSATSPCRG 307
M NV+NPIVIDQNYCP + CP Q SGIKIS VTY +I GTSA+++A+KFDCS +PC
Sbjct: 297 MTNVQNPIVIDQNYCPDNKNCPGQESGIKISDVTYQDIHGTSATEMAVKFDCSKRNPCTR 356
Query: 308 IGLKDIQLTYQRQPAQSFCRNIHGIARGVITPPSCL 343
I L+D++LTY+ QPA + C+N G A G + P SCL
Sbjct: 357 IKLEDVKLTYKNQPADASCKNADGTASGFVQPTSCL 392