BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Eca_sc000102.1_g0850.1
         (1994 letters)

Database: ./nr 
           95,329,361 sequences; 35,143,497,570 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_010251520.1 PREDICTED: vacuolar protein sorting-associated pr...  2449   0.0  
XP_010251529.1 PREDICTED: vacuolar protein sorting-associated pr...  2448   0.0  
XP_002267626.3 PREDICTED: vacuolar protein sorting-associated pr...  2306   0.0  

>XP_010251520.1 PREDICTED: vacuolar protein sorting-associated protein 8 homolog
            isoform X1 [Nelumbo nucifera]
          Length = 2063

 Score = 2449 bits (6346), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 1324/2064 (64%), Positives = 1561/2064 (75%), Gaps = 89/2064 (4%)

Query: 1    MMTKKLNRPPMALDLDSFLELQTSDDEDDLNNFPHRTVDEILNDSDSSSLSSSPTSSHTI 60
            MMT+ L+RP   LDLDSFL++ +S D+DDL+  PHRTVDEILNDSDSSS SSS       
Sbjct: 6    MMTEMLSRPSGDLDLDSFLQIHSSSDDDDLSGVPHRTVDEILNDSDSSSSSSSTL----F 61

Query: 61   TSATEILHQQRISDLSLSRKDSSILSKNEIPSQDEDVSSISSAETTSTSRVSSTVDETLK 120
             SA     + R S++  S  + S   K E    D+D +S+ S E+ S SR+S  VDETLK
Sbjct: 62   DSAA--FRRGRFSNIPTSGNNLSSFPKEESQIHDDD-ASVCSHESLSVSRIS-IVDETLK 117

Query: 121  SPD---SSKLEERFVDSRVSSFNRRSSGEYSGNSLFLGRSLSRPLPPLFGSSKPNPKPGA 177
            S +   S++LEE+ +DS  SS  R  S E SGN+  LGRS SRP  PLFG  K NPKPGA
Sbjct: 118  SREALNSAQLEEKSIDSNTSSLRRIRSDEPSGNTFSLGRSASRPFSPLFGGVKANPKPGA 177

Query: 178  ALAAAAAASRSIPTPHAAAIKSRR-----ERKSLQTEEFSTLIGASEIELDNNNANFDGL 232
            ALAAAAAASRSIPTPHAAAIKSRR     +RK L TEE    +G     LD N A+ D  
Sbjct: 178  ALAAAAAASRSIPTPHAAAIKSRRASSSIQRKLLHTEELDNSVGELNTFLDGN-AHVDEP 236

Query: 233  PSLNDASSSVRSESREIETNLGEEDVKGENFLSSPVETVV-----GEHSYENGSEGYVVA 287
                 A     S    +   L EED K   F SS  E++V     G+   EN  E   V+
Sbjct: 237  SHSGGACDGFGSGIILVGRKLSEEDEKVRKFPSSSAESIVLEFCGGDEVTENSHESNEVS 296

Query: 288  EVLPEETEAISAKETSVGQVEVSE------------LVEIRSVLDIADSNEDTTNLDEKS 335
             +   + E   A E+    +   E            +VE ++   I  +  +  + DEKS
Sbjct: 297  CLKDMQIERAQASESDGTGLLSQENLSNLNSSTSTSIVEPQATSPIGTA--EVLDTDEKS 354

Query: 336  STLDFTHVNEDHIPAPVAITEDAGKLN-----STAYAEEQNLSSCPEHEGATYLED---L 387
               + T++ +++ P+  +  E+A K +     S + + E+++ S P +E A  L+D   +
Sbjct: 355  EISNSTNIKKENHPS-FSTNENAHKEDLSSNVSDSISLEKDIPSSPRYEKAKRLQDDLVV 413

Query: 388  NSLRL----DTITDSAKEGEETLAVDDTK-KSDTTELV-DQILVRDSKKSNRRNGKSFM- 440
              L +     +++   K+GE +   DDT  +S  TELV D+ L  +SK+ +++  K    
Sbjct: 414  QGLGIHDSEKSVSSDTKDGEVSFVGDDTSSRSGITELVEDKFLQLESKRVSKKTAKKLRP 473

Query: 441  --KPLELAEELEKRHAFSGLDWEEGVAAQPMRLEGIRRGPPAVGYLQIDPDNMITRTISS 498
              KPLELAEELEK+HA SGL WEEG AAQPMRLEGIRRGPPAVGYL IDPDN ITR ISS
Sbjct: 474  SKKPLELAEELEKKHASSGLHWEEGAAAQPMRLEGIRRGPPAVGYLHIDPDNAITRAISS 533

Query: 499  QAFRRDHGSAKVVAVHANFIAVGMSKGLVLVAPSKYSPHYADHMDTKMLYLGSQGDKSVS 558
            QAFRRDHGS +V+AVHANFIAVGMSKG+++V PSKYS H AD+MD KM  LG+ G+KS S
Sbjct: 534  QAFRRDHGSPQVLAVHANFIAVGMSKGVIIVVPSKYSAHSADNMDYKMSILGAHGEKSHS 593

Query: 559  PVTSMCFNQQGDLLLVGYGDGHITLWDIPRAAVAKLIAGEHTAPVVHTLFLGQDFQVTRN 618
            PVTSMCFNQQGDLLL GYGDGHIT+WD+ R AVAK+I G HTAPVVHTLFLGQD QVTR 
Sbjct: 594  PVTSMCFNQQGDLLLAGYGDGHITVWDVQREAVAKVITGGHTAPVVHTLFLGQDSQVTRQ 653

Query: 619  FKAVTGDCKGLVLLHTFSVVPLLNRFSNNTQCLLDGQRTGTVLSASPLLIDVS--GGPLT 676
            FKAVTGDCKGLVLLH FSV PL N+ +  TQCLLDGQRTGTVL+ASPLL+  S  G P+ 
Sbjct: 654  FKAVTGDCKGLVLLHAFSVFPLFNKITIKTQCLLDGQRTGTVLTASPLLLYESHEGNPI- 712

Query: 677  SGPGNATASSSGIGSMMGGVVGGVVGAEARWKLFSEASSLVEEGVVIFVTHQTALVVRLT 736
            S  GNAT S+SGIGSMMGGVVGGVVG EA WK+ SE SSLV+EGVVIFVTHQTALV RL+
Sbjct: 713  SAQGNATTSASGIGSMMGGVVGGVVGGEAGWKILSEGSSLVDEGVVIFVTHQTALVARLS 772

Query: 737  PNLEVYAQLPKPDGVREGSMPYTAWKCTMQ----TTENVSTETSDKASLLAIAWDRKVQV 792
            P LEVYAQL KPDGVREGSMPYTAWKC  Q    +TE+   ET +K SLLAIAWDRK+QV
Sbjct: 773  PTLEVYAQLSKPDGVREGSMPYTAWKCMTQPQGSSTESFPGETFEKVSLLAIAWDRKIQV 832

Query: 793  AKLVKSELKVYREWTLDSAAIGVAWLDDQMLVVVTLRGHLCLFAKEGTELHRSSFSANGS 852
            AKLVKSELK+Y+EWTLDS AIGV WLDDQMLVV+TLRG LCLFAKEGTELHR+SF+ +GS
Sbjct: 833  AKLVKSELKIYKEWTLDSVAIGVQWLDDQMLVVLTLRGQLCLFAKEGTELHRTSFAVDGS 892

Query: 853  GADDLIAYHTYFSNVFGNPEKAYHNCIAVRGATVYIIGSTQLVISRLLPWKERIQVLQKA 912
            G  D+I YHTYF+NVFGNPEKAYHNC+A RGA++Y++G   LV+SRLLPWKERIQVL++A
Sbjct: 893  GGVDIITYHTYFTNVFGNPEKAYHNCVAARGASIYMLGPMHLVVSRLLPWKERIQVLRRA 952

Query: 913  GDWMGALDMAMRLYDGQAHGVIDLPRTVDAIREAIMPYLVELVLSYVDEVFSYMSVAFCN 972
            GDWMGALDMAMRLYDG AHGVIDLPRT+DAI+E IMPYLVEL+LSYVDEVFSY+SVAFCN
Sbjct: 953  GDWMGALDMAMRLYDGHAHGVIDLPRTLDAIQETIMPYLVELLLSYVDEVFSYISVAFCN 1012

Query: 973  QIGR-EQLDDSVSISSSVQIEMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKFVAVKHG 1031
            QI + EQ++D  SI SSV  EMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKF+AVKHG
Sbjct: 1013 QIEKVEQVNDPKSIRSSVHSEMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKFMAVKHG 1072

Query: 1032 GTFLELLEPYILKDMLGCLPPEIMQALVEHYSSKGWLQRVEQCVLHMDISSLDFNQVVRL 1091
            GTFLELLEPYILKDMLGCLPPEIMQALVEHYS KGWLQRVEQCVLHMDISSLDFNQVVRL
Sbjct: 1073 GTFLELLEPYILKDMLGCLPPEIMQALVEHYSEKGWLQRVEQCVLHMDISSLDFNQVVRL 1132

Query: 1092 CREHGLYGALIYLFNKGLDDYRAPLEELLLVLQNKHIENASAIGYRMLVYLKYCFTGLAF 1151
            C+EHGLYGALIYLFN+GLDD++APLEELL VL+N   ++A AIGYR+LVYLKYCF+GLAF
Sbjct: 1133 CQEHGLYGALIYLFNRGLDDFKAPLEELLQVLRNSQGDDAVAIGYRILVYLKYCFSGLAF 1192

Query: 1152 PPGHGEISPTRLPSVRRELVHFLLEDSVAKLSDVVKGFKPPTGAYPNLYHLLWLDTEATL 1211
            PPGHG I PTRLPS+R EL+ FL+EDS    S+VV G K  TG  PNLY LL LDTEATL
Sbjct: 1193 PPGHGSIPPTRLPSLRAELMQFLIEDSNDLNSEVVAGTKSSTGTCPNLYPLLLLDTEATL 1252

Query: 1212 DVLRLAFLEEVSNVNLVLDDGGVEDNDKNNGTSKENQ------NLMVQHTVNALTCIVDS 1265
             V+  AFLEE     +   D     +D N+   KEN       +LMVQ+TV+ L  I+D 
Sbjct: 1253 QVISCAFLEE----EVPRSDHSFHGSDTNSEDVKENDPKIESLDLMVQNTVDTLIHILDL 1308

Query: 1266 DILGVDHSSPV-DTGSPEVWPSSKDKGHLLEFIACFVAYERATISEPVLSHVLEFLTSES 1324
            +I  V+ SS + DTG PE+WPS KD  HLLEFIA FVA ++AT+S+ VLSH+LE+LTSES
Sbjct: 1309 EISEVERSSGIDDTGYPEIWPSKKDMAHLLEFIAYFVACKQATVSKSVLSHILEYLTSES 1368

Query: 1325 P------NQKNETSKRREKQVLALLRVVPETDWDSFHLLHLCEKAQYYQVCGLIHTARGQ 1378
                   +QK ET KRREK V+ALL+VVPET+WDS ++LHLCEKA+++QVCGLIH  RGQ
Sbjct: 1369 SLSLSVHHQKTETLKRREKHVIALLKVVPETNWDSSYVLHLCEKAEFHQVCGLIHARRGQ 1428

Query: 1379 HVDALDSYLKDREEPVHAFSFINNMLLQLRDNNLATFRSAVISRVPELVTLSREGTFFLT 1438
            ++ ALDSYLKD +EP+HAFSFIN+ML  LRD     F+SAVISR+P+LV LSREG FFL 
Sbjct: 1429 YIAALDSYLKDTDEPIHAFSFINDMLRLLRDTESTQFQSAVISRIPDLVNLSREGAFFLV 1488

Query: 1439 IDHFHKESQTILSELHPHPKSLFLYLKTTIEVHLSGRLDFSAMEKEYVLDVPNGRRVKDE 1498
            I+HF+KE   IL+ L  HPKSLFLYLKT IE+HL+G L+FS++EK   LDV  G+R+KD 
Sbjct: 1489 IEHFNKEYHQILAGLRSHPKSLFLYLKTIIEIHLAGTLNFSSLEKGDNLDVSCGKRLKDH 1548

Query: 1499 KV--ESYLERISGFPKLLQHKSISVTDEVVELYLQLLCQYERHSVLKFLETFESYRVENC 1556
                E+YL RIS FPKLL+   + VTDE++ELYL+LLCQYER SVLKFLETFESYRVE+C
Sbjct: 1549 TNGHEAYLARISDFPKLLRQNPVHVTDEMIELYLELLCQYERQSVLKFLETFESYRVEHC 1608

Query: 1557 LRLCQEYQVIDAAAFLLERVGDVGSALLLTLSGLNENFRTLEAAVENIVCSSP----TEK 1612
            LRLCQEY VIDAAAFLLERVGDVGSALLLTLSGLNE F  L+AAVE I+   P    TE 
Sbjct: 1609 LRLCQEYGVIDAAAFLLERVGDVGSALLLTLSGLNEKFTILDAAVERIISDIPLSGTTEI 1668

Query: 1613 EQLNTILMMGEVNSIRDILHASIGLCQRNTVRLDPEESESLWFQLLDSFCEPLRDSYDGK 1672
            EQLN++L M EV++I DIL  SIGLCQRNT RLDP ESESLWF LLDSFCEPL+DSYD +
Sbjct: 1669 EQLNSVLRMEEVDAIHDILQTSIGLCQRNTQRLDPNESESLWFHLLDSFCEPLKDSYDSQ 1728

Query: 1673 MPLE-GKYASKTKEMFDWQDDREAATYKWRISQSHKGAHIFRKVFAQFIKEIVEGMIGYL 1731
               E G + S     F   +D+ A+  KWRIS SH+GAH+ R+V +QFI++IVEGMIGY+
Sbjct: 1729 TASEGGNHVSILAASFGTAEDKGASMNKWRISNSHRGAHVLRRVISQFIRKIVEGMIGYV 1788

Query: 1732 RLPSIMAKLLSDNGDQEFGDFKLTILGMLGTYGFERRILDTAKSLIEEDTFYTMSLLKKG 1791
            RLP+IM KLLSDNG QEFGDFKLTILGMLGTYGFERRIL TAKSLIE+DTFYTMSLLKKG
Sbjct: 1789 RLPTIMTKLLSDNGGQEFGDFKLTILGMLGTYGFERRILVTAKSLIEDDTFYTMSLLKKG 1848

Query: 1792 ASHGYAPQSLLCSICNYSLTKKHSTSGIRVFNCGHATHLQCEFQETESLNGDSSAGCPVC 1851
            ASHGYAPQSLLC +CN  L+K+ S+S IRVFNCGHATHLQCEFQE E+L   SS GCPVC
Sbjct: 1849 ASHGYAPQSLLCCLCNSVLSKESSSSSIRVFNCGHATHLQCEFQENEALEVGSSVGCPVC 1908

Query: 1852 LPRKKKQRSRNKSVITNNGLVKSSLLKPKRTQGTTGITQHSYESDA-EKPYGLQQISRFE 1910
            +P+KK ++SR+KSV  ++GLVKSSL + +  +GTT I QH YE +A EKPYGLQQISRFE
Sbjct: 1909 MPKKKSRQSRSKSVHEDSGLVKSSLSRTQHARGTT-IIQHPYEPEALEKPYGLQQISRFE 1967

Query: 1911 ILNNLQKVQRTFQLENLPPLKLAPPTVYHEKVKKRSISLTGETSSS-VKSEKTNKIKQLK 1969
            ILNNLQK +++ QLENLP L+L PP +YHEKVKK      GE+SS+  K EK +K KQ +
Sbjct: 1968 ILNNLQKGKKSVQLENLPQLRLVPPAIYHEKVKKGMDIFAGESSSTPPKGEKPSKSKQFR 2027

Query: 1970 DLKTKGSSLRFPLKSSIFGNEKAR 1993
            +LK KGS+LRFPL+S+IF   + +
Sbjct: 2028 ELKMKGSTLRFPLRSNIFVQRRGQ 2051


>XP_010251529.1 PREDICTED: vacuolar protein sorting-associated protein 8 homolog
            isoform X2 [Nelumbo nucifera]
          Length = 2051

 Score = 2448 bits (6344), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 1325/2064 (64%), Positives = 1560/2064 (75%), Gaps = 89/2064 (4%)

Query: 1    MMTKKLNRPPMALDLDSFLELQTSDDEDDLNNFPHRTVDEILNDSDSSSLSSSPTSSHTI 60
            MMT+ L+RP   LDLDSFL++ +S D+DDL+  PHRTVDEILNDSDSSS SSS       
Sbjct: 6    MMTEMLSRPSGDLDLDSFLQIHSSSDDDDLSGVPHRTVDEILNDSDSSSSSSSTL----F 61

Query: 61   TSATEILHQQRISDLSLSRKDSSILSKNEIPSQDEDVSSISSAETTSTSRVSSTVDETLK 120
             SA     + R S++  S  + S   K E    D+D +S+ S E+ S SR+S  VDETLK
Sbjct: 62   DSAA--FRRGRFSNIPTSGNNLSSFPKEESQIHDDD-ASVCSHESLSVSRIS-IVDETLK 117

Query: 121  SPD---SSKLEERFVDSRVSSFNRRSSGEYSGNSLFLGRSLSRPLPPLFGSSKPNPKPGA 177
            S +   S++LEE+ +DS  SS  R  S E SGN+  LGRS SRP  PLFG  K NPKPGA
Sbjct: 118  SREALNSAQLEEKSIDSNTSSLRRIRSDEPSGNTFSLGRSASRPFSPLFGGVKANPKPGA 177

Query: 178  ALAAAAAASRSIPTPHAAAIKSRR-----ERKSLQTEEFSTLIGASEIELDNNNANFDGL 232
            ALAAAAAASRSIPTPHAAAIKSRR     +RK L TEE    +G     LD N A+ D  
Sbjct: 178  ALAAAAAASRSIPTPHAAAIKSRRASSSIQRKLLHTEELDNSVGELNTFLDGN-AHVDEP 236

Query: 233  PSLNDASSSVRSESREIETNLGEEDVKGENFLSSPVETVV-----GEHSYENGSEGYVVA 287
                 A     S    +   L EED K   F SS  E++V     G+   EN  E   V+
Sbjct: 237  SHSGGACDGFGSGIILVGRKLSEEDEKVRKFPSSSAESIVLEFCGGDEVTENSHESNEVS 296

Query: 288  EVLPEETEAISAKETSVGQVEVSE------------LVEIRSVLDIADSNEDTTNLDEKS 335
             +   + E   A E+    +   E            +VE ++   I  +  +  + DEKS
Sbjct: 297  CLKDMQIERAQASESDGTGLLSQENLSNLNSSTSTSIVEPQATSPIGTA--EVLDTDEKS 354

Query: 336  STLDFTHVNEDHIPAPVAITEDAGKLN-----STAYAEEQNLSSCPEHEGATYLED---L 387
               + T++ +++ P+  +  E+A K +     S + + E+++ S P +E A  L+D   +
Sbjct: 355  EISNSTNIKKENHPS-FSTNENAHKEDLSSNVSDSISLEKDIPSSPRYEKAKRLQDDLVV 413

Query: 388  NSLRL----DTITDSAKEGEETLAVDDTK-KSDTTELV-DQILVRDSKKSNRRNGKSFM- 440
              L +     +++   K+GE +   DDT  +S  TELV D+ L  +SK+ +++  K    
Sbjct: 414  QGLGIHDSEKSVSSDTKDGEVSFVGDDTSSRSGITELVEDKFLQLESKRVSKKTAKKLRP 473

Query: 441  --KPLELAEELEKRHAFSGLDWEEGVAAQPMRLEGIRRGPPAVGYLQIDPDNMITRTISS 498
              KPLELAEELEK+HA SGL WEEG AAQPMRLEGIRRGPPAVGYL IDPDN ITR ISS
Sbjct: 474  SKKPLELAEELEKKHASSGLHWEEGAAAQPMRLEGIRRGPPAVGYLHIDPDNAITRAISS 533

Query: 499  QAFRRDHGSAKVVAVHANFIAVGMSKGLVLVAPSKYSPHYADHMDTKMLYLGSQGDKSVS 558
            QAFRRDHGS +V+AVHANFIAVGMSKG+++V PSKYS H AD+MD KM  LG+ G+KS S
Sbjct: 534  QAFRRDHGSPQVLAVHANFIAVGMSKGVIIVVPSKYSAHSADNMDYKMSILGAHGEKSHS 593

Query: 559  PVTSMCFNQQGDLLLVGYGDGHITLWDIPRAAVAKLIAGEHTAPVVHTLFLGQDFQVTRN 618
            PVTSMCFNQQGDLLL GYGDGHIT+WD+ R AVAK+I G HTAPVVHTLFLGQD QVTR 
Sbjct: 594  PVTSMCFNQQGDLLLAGYGDGHITVWDVQREAVAKVITGGHTAPVVHTLFLGQDSQVTRQ 653

Query: 619  FKAVTGDCKGLVLLHTFSVVPLLNRFSNNTQCLLDGQRTGTVLSASPLLIDVS--GGPLT 676
            FKAVTGDCKGLVLLH FSV PL N+ +  TQCLLDGQRTGTVL+ASPLL+  S  G P+ 
Sbjct: 654  FKAVTGDCKGLVLLHAFSVFPLFNKITIKTQCLLDGQRTGTVLTASPLLLYESHEGNPI- 712

Query: 677  SGPGNATASSSGIGSMMGGVVGGVVGAEARWKLFSEASSLVEEGVVIFVTHQTALVVRLT 736
            S  GNAT S+SGIGSMMGGVVGGVVG EA WK+ SE SSLV+EGVVIFVTHQTALV RL+
Sbjct: 713  SAQGNATTSASGIGSMMGGVVGGVVGGEAGWKILSEGSSLVDEGVVIFVTHQTALVARLS 772

Query: 737  PNLEVYAQLPKPDGVREGSMPYTAWKCTMQ----TTENVSTETSDKASLLAIAWDRKVQV 792
            P LEVYAQL KPDGVREGSMPYTAWKC  Q    +TE+   ET +K SLLAIAWDRK+QV
Sbjct: 773  PTLEVYAQLSKPDGVREGSMPYTAWKCMTQPQGSSTESFPGETFEKVSLLAIAWDRKIQV 832

Query: 793  AKLVKSELKVYREWTLDSAAIGVAWLDDQMLVVVTLRGHLCLFAKEGTELHRSSFSANGS 852
            AKLVKSELK+Y+EWTLDS AIGV WLDDQMLVV+TLRG LCLFAKEGTELHR+SF+ +GS
Sbjct: 833  AKLVKSELKIYKEWTLDSVAIGVQWLDDQMLVVLTLRGQLCLFAKEGTELHRTSFAVDGS 892

Query: 853  GADDLIAYHTYFSNVFGNPEKAYHNCIAVRGATVYIIGSTQLVISRLLPWKERIQVLQKA 912
            G  D+I YHTYF+NVFGNPEKAYHNC+A RGA++Y++G   LV+SRLLPWKERIQVL++A
Sbjct: 893  GGVDIITYHTYFTNVFGNPEKAYHNCVAARGASIYMLGPMHLVVSRLLPWKERIQVLRRA 952

Query: 913  GDWMGALDMAMRLYDGQAHGVIDLPRTVDAIREAIMPYLVELVLSYVDEVFSYMSVAFCN 972
            GDWMGALDMAMRLYDG AHGVIDLPRT+DAI+E IMPYLVEL+LSYVDEVFSY+SVAFCN
Sbjct: 953  GDWMGALDMAMRLYDGHAHGVIDLPRTLDAIQETIMPYLVELLLSYVDEVFSYISVAFCN 1012

Query: 973  QIGR-EQLDDSVSISSSVQIEMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKFVAVKHG 1031
            QI + EQ++D  SI SSV  EMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKF+AVKHG
Sbjct: 1013 QIEKVEQVNDPKSIRSSVHSEMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKFMAVKHG 1072

Query: 1032 GTFLELLEPYILKDMLGCLPPEIMQALVEHYSSKGWLQRVEQCVLHMDISSLDFNQVVRL 1091
            GTFLELLEPYILKDMLGCLPPEIMQALVEHYS KGWLQRVEQCVLHMDISSLDFNQVVRL
Sbjct: 1073 GTFLELLEPYILKDMLGCLPPEIMQALVEHYSEKGWLQRVEQCVLHMDISSLDFNQVVRL 1132

Query: 1092 CREHGLYGALIYLFNKGLDDYRAPLEELLLVLQNKHIENASAIGYRMLVYLKYCFTGLAF 1151
            C+EHGLYGALIYLFN+GLDD++APLEELL VL+N   ++A AIGYR+LVYLKYCF+GLAF
Sbjct: 1133 CQEHGLYGALIYLFNRGLDDFKAPLEELLQVLRNSQGDDAVAIGYRILVYLKYCFSGLAF 1192

Query: 1152 PPGHGEISPTRLPSVRRELVHFLLEDSVAKLSDVVKGFKPPTGAYPNLYHLLWLDTEATL 1211
            PPGHG I PTRLPS+R EL+ FL+EDS    S+VV G K  TG  PNLY LL LDTEATL
Sbjct: 1193 PPGHGSIPPTRLPSLRAELMQFLIEDSNDLNSEVVAGTKSSTGTCPNLYPLLLLDTEATL 1252

Query: 1212 DVLRLAFLEEVSNVNLVLDDGGVEDNDKNNGTSKENQ------NLMVQHTVNALTCIVDS 1265
             V+  AFLEE     +   D     +D N+   KEN       +LMVQ+TV+ L  I+D 
Sbjct: 1253 QVISCAFLEE----EVPRSDHSFHGSDTNSEDVKENDPKIESLDLMVQNTVDTLIHILDL 1308

Query: 1266 DILGVDHSSPV-DTGSPEVWPSSKDKGHLLEFIACFVAYERATISEPVLSHVLEFLTSES 1324
            +I  V+ SS + DTG PE+WPS KD  HLLEFIA FVA ++AT+S+ VLSH+LE+LTSES
Sbjct: 1309 EISEVERSSGIDDTGYPEIWPSKKDMAHLLEFIAYFVACKQATVSKSVLSHILEYLTSES 1368

Query: 1325 P------NQKNETSKRREKQVLALLRVVPETDWDSFHLLHLCEKAQYYQVCGLIHTARGQ 1378
                   +QK ET KRREK V+ALL+VVPET+WDS ++LHLCEKA+++QVCGLIH  RGQ
Sbjct: 1369 SLSLSVHHQKTETLKRREKHVIALLKVVPETNWDSSYVLHLCEKAEFHQVCGLIHARRGQ 1428

Query: 1379 HVDALDSYLKDREEPVHAFSFINNMLLQLRDNNLATFRSAVISRVPELVTLSREGTFFLT 1438
            ++ ALDSYLKD +EP+HAFSFIN+ML  LRD     F+SAVISR+P+LV LSREG FFL 
Sbjct: 1429 YIAALDSYLKDTDEPIHAFSFINDMLRLLRDTESTQFQSAVISRIPDLVNLSREGAFFLV 1488

Query: 1439 IDHFHKESQTILSELHPHPKSLFLYLKTTIEVHLSGRLDFSAMEKEYVLDVPNGRRVKDE 1498
            I+HF+KE   IL+ L  HPKSLFLYLKT IE+HL+G L+FS++EK   LDV  G+R+KD 
Sbjct: 1489 IEHFNKEYHQILAGLRSHPKSLFLYLKTIIEIHLAGTLNFSSLEKGDNLDVSCGKRLKDH 1548

Query: 1499 KV--ESYLERISGFPKLLQHKSISVTDEVVELYLQLLCQYERHSVLKFLETFESYRVENC 1556
                E+YL RIS FPKLL+   + VTDE++ELYL+LLCQYER SVLKFLETFESYRVE+C
Sbjct: 1549 TNGHEAYLARISDFPKLLRQNPVHVTDEMIELYLELLCQYERQSVLKFLETFESYRVEHC 1608

Query: 1557 LRLCQEYQVIDAAAFLLERVGDVGSALLLTLSGLNENFRTLEAAVENIVCSSP----TEK 1612
            LRLCQEY VIDAAAFLLERVGDVGSALLLTLSGLNE F  L+AAVE I+   P    TE 
Sbjct: 1609 LRLCQEYGVIDAAAFLLERVGDVGSALLLTLSGLNEKFTILDAAVERIISDIPLSGTTEI 1668

Query: 1613 EQLNTILMMGEVNSIRDILHASIGLCQRNTVRLDPEESESLWFQLLDSFCEPLRDSYDGK 1672
            EQLN++L M EV++I DIL  SIGLCQRNT RLDP ESESLWF LLDSFCEPL+DSYD +
Sbjct: 1669 EQLNSVLRMEEVDAIHDILQTSIGLCQRNTQRLDPNESESLWFHLLDSFCEPLKDSYDSQ 1728

Query: 1673 MPLE-GKYASKTKEMFDWQDDREAATYKWRISQSHKGAHIFRKVFAQFIKEIVEGMIGYL 1731
               E G + S     F   +D+ A+  KWRIS SH+GAH+ R+V +QFI++IVEGMIGY+
Sbjct: 1729 TASEGGNHVSILAASFGTAEDKGASMNKWRISNSHRGAHVLRRVISQFIRKIVEGMIGYV 1788

Query: 1732 RLPSIMAKLLSDNGDQEFGDFKLTILGMLGTYGFERRILDTAKSLIEEDTFYTMSLLKKG 1791
            RLP+IM KLLSDNG QEFGDFKLTILGMLGTYGFERRIL TAKSLIE+DTFYTMSLLKKG
Sbjct: 1789 RLPTIMTKLLSDNGGQEFGDFKLTILGMLGTYGFERRILVTAKSLIEDDTFYTMSLLKKG 1848

Query: 1792 ASHGYAPQSLLCSICNYSLTKKHSTSGIRVFNCGHATHLQCEFQETESLNGDSSAGCPVC 1851
            ASHGYAPQSLLC +CN  L+K+ S+S IRVFNCGHATHLQCEFQE E+L   SS GCPVC
Sbjct: 1849 ASHGYAPQSLLCCLCNSVLSKESSSSSIRVFNCGHATHLQCEFQENEALEVGSSVGCPVC 1908

Query: 1852 LPRKKKQRSRNKSVITNNGLVKSSLLKPKRTQGTTGITQHSYESDA-EKPYGLQQISRFE 1910
            +P+KK ++SR+KSV  ++GLVKSSL + +  +GTT I QH YE +A EKPYGLQQISRFE
Sbjct: 1909 MPKKKSRQSRSKSVHEDSGLVKSSLSRTQHARGTT-IIQHPYEPEALEKPYGLQQISRFE 1967

Query: 1911 ILNNLQKVQRTFQLENLPPLKLAPPTVYHEKVKKRSISLTGETSSS-VKSEKTNKIKQLK 1969
            ILNNLQK +++ QLENLP L+L PP +YHEKVKK      GE+SS+  K EK +K KQ +
Sbjct: 1968 ILNNLQKGKKSVQLENLPQLRLVPPAIYHEKVKKGMDIFAGESSSTPPKGEKPSKSKQFR 2027

Query: 1970 DLKTKGSSLRFPLKSSIFGNEKAR 1993
            +LK KGS+LRFPL+S+IF     R
Sbjct: 2028 ELKMKGSTLRFPLRSNIFEKRTKR 2051


>XP_002267626.3 PREDICTED: vacuolar protein sorting-associated protein 8 homolog
            isoform X1 [Vitis vinifera]
          Length = 1988

 Score = 2306 bits (5977), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 1278/2053 (62%), Positives = 1507/2053 (73%), Gaps = 126/2053 (6%)

Query: 2    MTKKLNRPPMALDLDSFLELQTSDDEDD-LNNFPHRTVDEILNDSDSSSLSSSPTSSHTI 60
            MTKKL+ PPM LDLDSF+ L + DD+DD LN  PHRTVDEILNDSDSSS S SP+    +
Sbjct: 1    MTKKLSAPPMELDLDSFIHLTSDDDDDDALNRVPHRTVDEILNDSDSSSSSLSPSDHSYL 60

Query: 61   TSATEILHQQRISDLSLSRKDSSILSKNEIPSQDEDVSSISSAETTSTSRVSSTVDETLK 120
                   H     D + SR               +DV S+S+ +T S  R  S   E+LK
Sbjct: 61   AK-----HSSLFEDANDSR---------------DDVVSVSTPKTLSDERPKSA--ESLK 98

Query: 121  SPDSSKLEERFVDSRVSSFNRRSSGEYSGNSLFLGRSLSRPLPPLFGSSKPNPKPGAALA 180
                +++E+R V  + +S +R  +G+ SG+S  LGR +SRPLPPLFGS + N KPGAALA
Sbjct: 99   F---NEIEDRLVQFKANSLSRVRTGDLSGDSFSLGRRVSRPLPPLFGSVRSNAKPGAALA 155

Query: 181  AAAAASRSIPTPHAAAIKSRRE-----RKSLQTEEFSTLIGASEIELDNNNANFDGLPSL 235
            AAAAASR +PTPHAAAIKSRR      ++ L TEE                +  D L S 
Sbjct: 156  AAAAASRPVPTPHAAAIKSRRAGSGALQRVLDTEEL-------------GGSGLDKLGSS 202

Query: 236  NDASSSVRSESREIETNLGEEDVKGENFLSSPVETVVGEHSYENGSEGYVVAEVLPEETE 295
            +D  +   SE    +   GEED K E+F S+ +E  V     +  S    + E    + E
Sbjct: 203  SDVLNGAGSEIASSDWKSGEEDDKFEDFQSATIEWTVKADVDDKVSVKDEIVESSHRDGE 262

Query: 296  AISAKETSVGQVEVSELVEIRSVLDIADSNEDTTNLDEKSSTLDFTHVNEDHIPAPVAIT 355
                ++     V   E  E R    + DS+E   N    SS        E  + A ++I 
Sbjct: 263  VFDLEKVPTEVVHTLEEDESR----VNDSDEILLN----SSA-------ETGLAASLSIE 307

Query: 356  EDAGKLN-----STAY-AEEQNLSSCPEHEGA---TYLEDLNSLR--------------- 391
            E++  LN     S +Y  ++QN++S    E A   T+L+  NS                 
Sbjct: 308  EESFDLNEGSAISGSYDVKDQNIASDNVEETASNSTFLDAANSADKDEKVREDLTLKTQD 367

Query: 392  LDTITDSAKEGEETLAVDD-TKKSDTTELVDQILVRDSKK--SNRRNGKSFMKPLELAEE 448
            L+ +   + +GE  +A DD + KSD TELV++ L +   K  S R   K  +KPLELAEE
Sbjct: 368  LEPVEPPSTDGEVNIAGDDWSPKSDVTELVEERLGQLESKMGSKRTEKKPRLKPLELAEE 427

Query: 449  LEKRHAFSGLDWEEGVAAQPMRLEGIRRGPPAVGYLQIDPDNMITRTISSQAFRRDHGSA 508
            LEK  A +GL WEEG AAQPMRLEG+RRG   +GY +ID +N ITRTISS AF+RDHGS 
Sbjct: 428  LEKSQASTGLHWEEGAAAQPMRLEGVRRGSTTLGYFEIDNNNTITRTISSPAFKRDHGSP 487

Query: 509  KVVAVHANFIAVGMSKGLVLVAPSKYSPHYADHMDTKMLYLGSQGDKSVSPVTSMCFNQQ 568
            +V+AVH NFIAVGMS+G+V+V PSKYS + AD+MD K+L LG QG++S +PVTSMCFN Q
Sbjct: 488  QVLAVHLNFIAVGMSRGVVMVVPSKYSAYNADNMDAKILMLGLQGERSHAPVTSMCFNHQ 547

Query: 569  GDLLLVGYGDGHITLWDIPRAAVAKLIAGEHTAPVVHTLFLGQDFQVTRNFKAVTGDCKG 628
            GDLLL GYGDGHIT+WD+ RA  AK+I GEH+APV+HTLFLGQD QVTR FKAVTGD KG
Sbjct: 548  GDLLLAGYGDGHITVWDVQRATAAKVITGEHSAPVIHTLFLGQDSQVTRQFKAVTGDSKG 607

Query: 629  LVLLHTFSVVPLLNRFSNNTQCLLDGQRTGTVLSASPLLID-VSGGPLTSGPGNATASSS 687
            LVLLH FSVVPLLNRFS  TQCLLDGQRTGTVLSASPLL+D  SG  L S  GNAT S+S
Sbjct: 608  LVLLHAFSVVPLLNRFSIKTQCLLDGQRTGTVLSASPLLLDESSGSSLMSSQGNATGSTS 667

Query: 688  GIGSMMGGVVGGVVGAEARWKLFSEASSLVEEGVVIFVTHQTALVVRLTPNLEVYAQLPK 747
             IGSMM    GGVVG +A WKLFSE SSLVEEGVVIFVTHQTALVVRL+P+LEVYAQL K
Sbjct: 668  SIGSMM----GGVVGGDAGWKLFSEGSSLVEEGVVIFVTHQTALVVRLSPSLEVYAQLNK 723

Query: 748  PDGVREGSMPYTAWKCTM-----QTTENVSTETSDKASLLAIAWDRKVQVAKLVKSELKV 802
            PDGVREGSMPYTAWKC        +TEN   E S++ SLLAIAWDRKVQVAKLVKSELK+
Sbjct: 724  PDGVREGSMPYTAWKCMTIHSRGLSTENTPVEASERVSLLAIAWDRKVQVAKLVKSELKI 783

Query: 803  YREWTLDSAAIGVAWLDDQMLVVVTLRGHLCLFAKEGTELHRSSFSANGSGADDLIAYHT 862
            Y +WTL+S AIGVAWLDDQ+LVV+T  G LCLFAK+GT +H++SF+ +GSG DD +AYHT
Sbjct: 784  YGKWTLESTAIGVAWLDDQILVVLTSTGQLCLFAKDGTVIHQTSFAVDGSGGDDPVAYHT 843

Query: 863  YFSNVFGNPEKAYHNCIAVRGATVYIIGSTQLVISRLLPWKERIQVLQKAGDWMGALDMA 922
            YF+N+FGNPEKAY N IAVRGA++YI+G   LV+SRLL WKERIQVL+KAGDWMGAL+MA
Sbjct: 844  YFTNIFGNPEKAYQNSIAVRGASIYILGPVHLVVSRLLTWKERIQVLRKAGDWMGALNMA 903

Query: 923  MRLYDGQAHGVIDLPRTVDAIREAIMPYLVELVLSYVDEVFSYMSVAFCNQIGR-EQLDD 981
            M LYDG +HGVIDLPR+++A++EAIMPYLVEL+LSYVDEVFSY+SVAFCNQIG+ EQLDD
Sbjct: 904  MTLYDGNSHGVIDLPRSLEAVQEAIMPYLVELLLSYVDEVFSYISVAFCNQIGKMEQLDD 963

Query: 982  SVSISSSVQIEMEEQFARVGGVAVEFCVHIKRIDILFDDIFSKFVAVKHGGTFLELLEPY 1041
              +  SSV  E++EQF RVGGVAVEFCVHIKR DILFD+IFSKFV V+H  TFLELLEPY
Sbjct: 964  PKNRGSSVHFEIKEQFTRVGGVAVEFCVHIKRTDILFDEIFSKFVGVQHRDTFLELLEPY 1023

Query: 1042 ILKDMLGCLPPEIMQALVEHYSSKGWLQRVEQCVLHMDISSLDFNQVVRLCREHGLYGAL 1101
            ILKDMLG LPPEIMQALVEHYSSKGWLQRVEQCVLHMDISSLDFNQVVRLCREHGLYGAL
Sbjct: 1024 ILKDMLGSLPPEIMQALVEHYSSKGWLQRVEQCVLHMDISSLDFNQVVRLCREHGLYGAL 1083

Query: 1102 IYLFNKGLDDYRAPLEELLLVLQNKHIENASAIGYRMLVYLKYCFTGLAFPPGHGEISPT 1161
            IYLFN+GLDD++APLEELL+VL N+  E+AS++GYRMLVYLKYCF+GLAFPPGHG + PT
Sbjct: 1084 IYLFNRGLDDFKAPLEELLVVLLNRPRESASSLGYRMLVYLKYCFSGLAFPPGHGTLPPT 1143

Query: 1162 RLPSVRRELVHFLLEDSVAKLSDVVKGFKPPTGAYPNLYHLLWLDTEATLDVLRLAFLE- 1220
            RLPS+R ELV FLLED  A  S  V      T A PNLYHLL LDTEATLDVLR AF+E 
Sbjct: 1144 RLPSLRTELVQFLLEDLNALNSQAVSSLS-STRALPNLYHLLELDTEATLDVLRYAFVED 1202

Query: 1221 EVSNVNLVLDDGGVEDNDKNNGTSK------ENQNLMVQHTVNALTCIVDSDILGVDHSS 1274
            E++  ++ L D      D N    K      E QNL+VQ+TVNAL  I+  DI   + SS
Sbjct: 1203 EITKPDVSLHDS----TDANMEAGKEIDLMGEIQNLLVQNTVNALIHIL--DISQKNRSS 1256

Query: 1275 -PVDTGSPEVWPSSKDKGHLLEFIACFVAYERATISEPVLSHVLEFLTSE------SPNQ 1327
               D GS E+WPS KD GHL EF+A +VA +RA +S+ VLS +LE+LTSE      S  +
Sbjct: 1257 GSSDIGSLELWPSKKDMGHLFEFVAYYVACKRANVSKTVLSQILEYLTSENKLPQSSSKE 1316

Query: 1328 KNETSKRREKQVLALLRVVPETDWDSFHLLHLCEKAQYYQVCGLIHTARGQHVDALDSYL 1387
               T KRREKQVLALL VVPE DWD+ ++LHLCEKA++YQVCGLIH+ R Q++ ALDSY+
Sbjct: 1317 SVGTLKRREKQVLALLEVVPEKDWDASYVLHLCEKAEFYQVCGLIHSIRHQYLTALDSYM 1376

Query: 1388 KDREEPVHAFSFINNMLLQLRDNNLATFRSAVISRVPELVTLSREGTFFLTIDHFHKESQ 1447
            KD +EPVHAFSFIN+ L QL D   A FRSAVISR+PELV LSREGTFFL IDHF+KES 
Sbjct: 1377 KDVDEPVHAFSFINHTLSQLSDTESAAFRSAVISRIPELVNLSREGTFFLIIDHFNKESP 1436

Query: 1448 TILSELHPHPKSLFLYLKTTIEVHLSGRLDFSAMEKEYVLDVPNGRRVKDE--KVESYLE 1505
             ILSEL  HPKSLFLYLKT IEVHLSG L+FS ++ +  +D   GRRVK++   +E+YLE
Sbjct: 1437 HILSELRSHPKSLFLYLKTVIEVHLSGTLNFSCLQNDDTMDASCGRRVKNQLYGLEAYLE 1496

Query: 1506 RISGFPKLLQHKSISVTDEVVELYLQLLCQYERHSVLKFLETFESYRVENCLRLCQEYQV 1565
            RI  FPKLL +  + VTDE++ELYL+LLCQYE  SVLKFLETFESYRVE+CLRLCQEY +
Sbjct: 1497 RILDFPKLLLNNPVHVTDEMIELYLELLCQYEHTSVLKFLETFESYRVEHCLRLCQEYGI 1556

Query: 1566 IDAAAFLLERVGDVGSALLLTLSGLNENFRTLEAAVENIVCSSPTEKEQLNTILMMGEVN 1625
            IDAAAFLLERVGDVGSALLLTLSGLN+ F  LE AV +I+    +  + LNT+L M EV+
Sbjct: 1557 IDAAAFLLERVGDVGSALLLTLSGLNDKFNVLETAVGSILSEKASSVDHLNTVLKMKEVS 1616

Query: 1626 SIRDILHASIGLCQRNTVRLDPEESESLWFQLLDSFCEPLRDSYDGKMPLE-GKYASKTK 1684
             I DILH  IGLCQRNT RL PEESESLWFQLLDSFCEPL DSYD K+  E  K      
Sbjct: 1617 DIYDILHTCIGLCQRNTPRLVPEESESLWFQLLDSFCEPLMDSYDDKIVSEVEKPVGILA 1676

Query: 1685 EMFDWQDDREAATYKWRISQSHKGAHIFRKVFAQFIKEIVEGMIGYLRLPSIMAKLLSDN 1744
            E  + Q   EA   KW I +SH+GAH+ R++F+QFIKEIVEGM+G++RLP IM+KLLSDN
Sbjct: 1677 ESLETQAGDEACLNKWSIPKSHQGAHLLRRLFSQFIKEIVEGMVGFVRLPVIMSKLLSDN 1736

Query: 1745 GDQEFGDFKLTILGMLGTYGFERRILDTAKSLIEEDTFYTMSLLKKGASHGYAPQSLLCS 1804
            G+QEFGDFK+TILGMLGTYGFERRILDTAKSLIE+DTFYTMSLLKKGASHGYAP+SL+C 
Sbjct: 1737 GNQEFGDFKVTILGMLGTYGFERRILDTAKSLIEDDTFYTMSLLKKGASHGYAPRSLICC 1796

Query: 1805 ICNYSLTKKHSTSGIRVFNCGHATHLQCEFQETESLNGDSSAGCPVCLPRKKKQRSRNKS 1864
            ICN   TK  S+S IRVFNCGHATHLQCE  E E+ N  SS GCPVCLP+KK QRSR+KS
Sbjct: 1797 ICNCLFTKNSSSSSIRVFNCGHATHLQCELLENEASNRSSSVGCPVCLPKKKTQRSRSKS 1856

Query: 1865 VITNNGLV-KSSLLKPKRTQGTTGITQHSYESDA-EKPYGLQQISRFEILNNLQKVQRTF 1922
            V+  NGLV K    K ++ QGT  I  H +E+D  E PYGLQQI RFEILNNLQK +R  
Sbjct: 1857 VLMENGLVSKVPSRKTQQAQGT--IVLHPHENDVLENPYGLQQIPRFEILNNLQKDKRAI 1914

Query: 1923 QLENLPPLKLAPPTVYHEKVKKRSISLTGETSSSV-KSEKTNKIKQLKDLKTKGSSLRFP 1981
            Q+ENLP L+LAPP VYHEKV K    LTGE+SS++ K EK +K +QL++LK KGSS+RFP
Sbjct: 1915 QIENLPQLRLAPPAVYHEKVAKGIDFLTGESSSALAKIEKPSKTRQLRELKEKGSSIRFP 1974

Query: 1982 LKSSIFGNEKARK 1994
            LKSSIFG EK  K
Sbjct: 1975 LKSSIFGKEKTSK 1987


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