BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000108.1_g3370.1
(862 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_009341355.1 PREDICTED: non-lysosomal glucosylceramidase-like ... 1248 0.0
XP_009341354.1 PREDICTED: non-lysosomal glucosylceramidase-like ... 1242 0.0
XP_009341356.1 PREDICTED: non-lysosomal glucosylceramidase-like ... 1242 0.0
>XP_009341355.1 PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Pyrus
x bretschneideri]
Length = 966
Score = 1248 bits (3228), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 606/911 (66%), Positives = 706/911 (77%), Gaps = 53/911 (5%)
Query: 1 MDSDEIVPSSFMPKIQEIIHMAPKALRLWHHVRDEVSRGQAVTVDPFYSRQVTGCHGVPL 60
++S P F ++EIIH+AP +RLW H+R+E + + +DPF R +T HGVPL
Sbjct: 54 LNSKGSAPLPFTLSLKEIIHLAPIGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPL 113
Query: 61 GGIGAGSIGRSYRGEFQRFQLFPTICEDKPVLANQFSVFISRSNGKRYATVLSPRTPEGV 120
GGIGAGSIGRSY G+FQR+QLFP CE+KPVLA+QFSVF+SR+NG++Y+TVL PR+P+ +
Sbjct: 114 GGIGAGSIGRSYSGDFQRWQLFPGRCEEKPVLADQFSVFVSRTNGEKYSTVLCPRSPDDL 173
Query: 121 KEVSTSGIGSWDWNLNGKNSTYHALYPRAWSVYDGEPDPELKIVCRQISPFIPHNYKESS 180
KE SGIGSWDWNL G NSTYHAL+PRAWSVYDGEPDP LKIVCRQISPFIPHNYKESS
Sbjct: 174 KESQVSGIGSWDWNLKGDNSTYHALFPRAWSVYDGEPDPALKIVCRQISPFIPHNYKESS 233
Query: 181 FPVA-----LSNSGETAANVSLLFTWTNSVGGDSESSGSHFNSRMPTKSGVHGVLLHHNT 235
PV+ L NSG+T+A+V+LLFTW NSVGG S SG H NSR K GVHGVLLHH T
Sbjct: 234 LPVSVFTYTLYNSGKTSADVTLLFTWANSVGGLSGISGHHSNSRALMKDGVHGVLLHHKT 293
Query: 236 KDGHSPVTFAIAAQETADVKVSECPCFLISGNSQGITAKDMWNEIKERGSFDHLVSDNTS 295
+G SPVTFAIAAQET V VSECPCF+ISG+S+G+TAKDMW+EIKE GSFD L S
Sbjct: 294 ANGLSPVTFAIAAQETDGVHVSECPCFVISGDSKGVTAKDMWSEIKEHGSFDRLNSAEMP 353
Query: 296 MPSEPGSSIGAAVAASVSVPSNSTRTVTFSLAWACPEVNFPSGNTYHRRYTKFYGIHTDA 355
+PSEPGSSIGAA+AASV+VPS RTVTFSLAW CPE F G YHRRYTKFYG H +A
Sbjct: 354 LPSEPGSSIGAAIAASVAVPSGEVRTVTFSLAWDCPEAKFRGGKAYHRRYTKFYGTHGEA 413
Query: 356 AANLAHDAIL---------------------------------------------DGSLP 370
AAN+AHDAIL DGS P
Sbjct: 414 AANIAHDAILEHRHWESQIEAWQRPVLEDKRLPEWYPVTLFNELYFLNSGGTVWTDGSPP 473
Query: 371 LRSLATSGERKFSINCSGSNIGTPTAEP-QNNISVGLSGKMISVLEEIQTPVPSNSAFGS 429
+ SL + ERKFS++ S + + P QN+ ++ + G+M S LE++ TP+ +NSAFG+
Sbjct: 474 VHSLRSIIERKFSLDKSSLGLKSIIDAPEQNDTAIDILGRMTSTLEQVHTPIAANSAFGT 533
Query: 430 SLLQKGEENIGQFLYLEGIEYHMWNTCDVHFYSSFALIMLFPKLELSIQRDFAAAVMMHD 489
+LLQ+GEENIGQFLYLEGIEY MWNT DVHFYSSFAL+MLFPKL+LSIQRDFAAAVM+HD
Sbjct: 534 NLLQEGEENIGQFLYLEGIEYQMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMIHD 593
Query: 490 PERVRMLDDGNWAQRKALGAVPHDLGLNNPWIEVNAYNFHNTNRWKDLNPKFVLQIYRDV 549
P ++R+L DG W QRK LGAVPHD+GL++PW EVNAYN +NT+RWKDLNPKFVLQ+YRDV
Sbjct: 594 PSKMRLLCDGRWVQRKVLGAVPHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDV 653
Query: 550 IATGDKSFAQAVWPAVYTAMAYMDQFDKDNDGMIENEGFPDQTYDVWSVKGVSAYSGGLW 609
+ATGDK FA+AVWPAVY AMAYM+QFDKD DGMIEN+GFPDQTYD WSV GVSAYSGGLW
Sbjct: 654 VATGDKKFAEAVWPAVYVAMAYMEQFDKDGDGMIENDGFPDQTYDTWSVYGVSAYSGGLW 713
Query: 610 VAALQAASAMAREVGDEVSEEHFWLKFQKAKAVYGQLWNGEYFNYDNSGGISSSSIQADQ 669
VAALQAAS MAREVGD+ SE +FW KFQKAKAVY +LWNG YFNYDNSG SSSSIQADQ
Sbjct: 714 VAALQAASGMAREVGDKGSEVYFWQKFQKAKAVYAKLWNGSYFNYDNSGQASSSSIQADQ 773
Query: 670 LAGQWYARACQLLPIVDEDKAQSALGKVYNFNVLKTNNGKRGAVNGMRPDGTVDMAGMQS 729
LAGQWYARAC LLPIVDEDKA+SAL K+YN+NVLK +G+RGAVNGM P+G VDM+ +QS
Sbjct: 774 LAGQWYARACGLLPIVDEDKARSALEKIYNYNVLKFEDGRRGAVNGMLPNGKVDMSTLQS 833
Query: 730 REIWSGVTYAVAASMIHEGLEEMGFKTASGVYEASWSQEGLGYAFQTPEAWDTDDKYRCL 789
REIWSGVTYAVAASMI E +MGF TA G+YEA WS+EGLGY+FQTPEAW T +YR L
Sbjct: 834 REIWSGVTYAVAASMIQEDAIDMGFHTAEGIYEAGWSKEGLGYSFQTPEAWTTSGEYRSL 893
Query: 790 GYMRPLAIWAMQWALFPPKLFKEETSELLKEDPPLLFRNIDEFPRVAKLLKLPKEEDTRS 849
YMRPLAIW+MQWAL P LFK+ET L+ D +L R+ F +VA+LLKLP EE +RS
Sbjct: 894 AYMRPLAIWSMQWALTKPPLFKQETE--LEVDEVILLRHKAGFSKVAQLLKLPPEESSRS 951
Query: 850 CLQFFYDYAFK 860
LQ +DY K
Sbjct: 952 ILQTVFDYTCK 962
>XP_009341354.1 PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Pyrus
x bretschneideri]
Length = 968
Score = 1242 bits (3214), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 606/913 (66%), Positives = 706/913 (77%), Gaps = 55/913 (6%)
Query: 1 MDSDEIVPSSFMPKIQEIIHMAPKALRLWHHVRDEVSRGQAVTVDPFYSRQVTGCHGVPL 60
++S P F ++EIIH+AP +RLW H+R+E + + +DPF R +T HGVPL
Sbjct: 54 LNSKGSAPLPFTLSLKEIIHLAPIGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPL 113
Query: 61 GGIGAGSIGRSYRGEFQRFQLFPTICEDKPVLANQFSVFISRSNGKRYATVLSPRTPEGV 120
GGIGAGSIGRSY G+FQR+QLFP CE+KPVLA+QFSVF+SR+NG++Y+TVL PR+P+ +
Sbjct: 114 GGIGAGSIGRSYSGDFQRWQLFPGRCEEKPVLADQFSVFVSRTNGEKYSTVLCPRSPDDL 173
Query: 121 KEVSTSGIGSWDWNLNGKNSTYHALYPRAWSVYDGEPDPELKIVCRQISPFIPHNYKESS 180
KE SGIGSWDWNL G NSTYHAL+PRAWSVYDGEPDP LKIVCRQISPFIPHNYKESS
Sbjct: 174 KESQVSGIGSWDWNLKGDNSTYHALFPRAWSVYDGEPDPALKIVCRQISPFIPHNYKESS 233
Query: 181 FPVA-----LSNSGETAANVSLLFTWTNSVGGDSESSGSHFNSRMPTKSGVHGVLLHHN- 234
PV+ L NSG+T+A+V+LLFTW NSVGG S SG H NSR K GVHGVLLHH
Sbjct: 234 LPVSVFTYTLYNSGKTSADVTLLFTWANSVGGLSGISGHHSNSRALMKDGVHGVLLHHKY 293
Query: 235 -TKDGHSPVTFAIAAQETADVKVSECPCFLISGNSQGITAKDMWNEIKERGSFDHLVSDN 293
T +G SPVTFAIAAQET V VSECPCF+ISG+S+G+TAKDMW+EIKE GSFD L S
Sbjct: 294 RTANGLSPVTFAIAAQETDGVHVSECPCFVISGDSKGVTAKDMWSEIKEHGSFDRLNSAE 353
Query: 294 TSMPSEPGSSIGAAVAASVSVPSNSTRTVTFSLAWACPEVNFPSGNTYHRRYTKFYGIHT 353
+PSEPGSSIGAA+AASV+VPS RTVTFSLAW CPE F G YHRRYTKFYG H
Sbjct: 354 MPLPSEPGSSIGAAIAASVAVPSGEVRTVTFSLAWDCPEAKFRGGKAYHRRYTKFYGTHG 413
Query: 354 DAAANLAHDAIL---------------------------------------------DGS 368
+AAAN+AHDAIL DGS
Sbjct: 414 EAAANIAHDAILEHRHWESQIEAWQRPVLEDKRLPEWYPVTLFNELYFLNSGGTVWTDGS 473
Query: 369 LPLRSLATSGERKFSINCSGSNIGTPTAEP-QNNISVGLSGKMISVLEEIQTPVPSNSAF 427
P+ SL + ERKFS++ S + + P QN+ ++ + G+M S LE++ TP+ +NSAF
Sbjct: 474 PPVHSLRSIIERKFSLDKSSLGLKSIIDAPEQNDTAIDILGRMTSTLEQVHTPIAANSAF 533
Query: 428 GSSLLQKGEENIGQFLYLEGIEYHMWNTCDVHFYSSFALIMLFPKLELSIQRDFAAAVMM 487
G++LLQ+GEENIGQFLYLEGIEY MWNT DVHFYSSFAL+MLFPKL+LSIQRDFAAAVM+
Sbjct: 534 GTNLLQEGEENIGQFLYLEGIEYQMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMI 593
Query: 488 HDPERVRMLDDGNWAQRKALGAVPHDLGLNNPWIEVNAYNFHNTNRWKDLNPKFVLQIYR 547
HDP ++R+L DG W QRK LGAVPHD+GL++PW EVNAYN +NT+RWKDLNPKFVLQ+YR
Sbjct: 594 HDPSKMRLLCDGRWVQRKVLGAVPHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYR 653
Query: 548 DVIATGDKSFAQAVWPAVYTAMAYMDQFDKDNDGMIENEGFPDQTYDVWSVKGVSAYSGG 607
DV+ATGDK FA+AVWPAVY AMAYM+QFDKD DGMIEN+GFPDQTYD WSV GVSAYSGG
Sbjct: 654 DVVATGDKKFAEAVWPAVYVAMAYMEQFDKDGDGMIENDGFPDQTYDTWSVYGVSAYSGG 713
Query: 608 LWVAALQAASAMAREVGDEVSEEHFWLKFQKAKAVYGQLWNGEYFNYDNSGGISSSSIQA 667
LWVAALQAAS MAREVGD+ SE +FW KFQKAKAVY +LWNG YFNYDNSG SSSSIQA
Sbjct: 714 LWVAALQAASGMAREVGDKGSEVYFWQKFQKAKAVYAKLWNGSYFNYDNSGQASSSSIQA 773
Query: 668 DQLAGQWYARACQLLPIVDEDKAQSALGKVYNFNVLKTNNGKRGAVNGMRPDGTVDMAGM 727
DQLAGQWYARAC LLPIVDEDKA+SAL K+YN+NVLK +G+RGAVNGM P+G VDM+ +
Sbjct: 774 DQLAGQWYARACGLLPIVDEDKARSALEKIYNYNVLKFEDGRRGAVNGMLPNGKVDMSTL 833
Query: 728 QSREIWSGVTYAVAASMIHEGLEEMGFKTASGVYEASWSQEGLGYAFQTPEAWDTDDKYR 787
QSREIWSGVTYAVAASMI E +MGF TA G+YEA WS+EGLGY+FQTPEAW T +YR
Sbjct: 834 QSREIWSGVTYAVAASMIQEDAIDMGFHTAEGIYEAGWSKEGLGYSFQTPEAWTTSGEYR 893
Query: 788 CLGYMRPLAIWAMQWALFPPKLFKEETSELLKEDPPLLFRNIDEFPRVAKLLKLPKEEDT 847
L YMRPLAIW+MQWAL P LFK+ET L+ D +L R+ F +VA+LLKLP EE +
Sbjct: 894 SLAYMRPLAIWSMQWALTKPPLFKQETE--LEVDEVILLRHKAGFSKVAQLLKLPPEESS 951
Query: 848 RSCLQFFYDYAFK 860
RS LQ +DY K
Sbjct: 952 RSILQTVFDYTCK 964
>XP_009341356.1 PREDICTED: non-lysosomal glucosylceramidase-like isoform X3 [Pyrus
x bretschneideri]
Length = 954
Score = 1242 bits (3213), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 606/913 (66%), Positives = 706/913 (77%), Gaps = 55/913 (6%)
Query: 1 MDSDEIVPSSFMPKIQEIIHMAPKALRLWHHVRDEVSRGQAVTVDPFYSRQVTGCHGVPL 60
++S P F ++EIIH+AP +RLW H+R+E + + +DPF R +T HGVPL
Sbjct: 40 LNSKGSAPLPFTLSLKEIIHLAPIGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPL 99
Query: 61 GGIGAGSIGRSYRGEFQRFQLFPTICEDKPVLANQFSVFISRSNGKRYATVLSPRTPEGV 120
GGIGAGSIGRSY G+FQR+QLFP CE+KPVLA+QFSVF+SR+NG++Y+TVL PR+P+ +
Sbjct: 100 GGIGAGSIGRSYSGDFQRWQLFPGRCEEKPVLADQFSVFVSRTNGEKYSTVLCPRSPDDL 159
Query: 121 KEVSTSGIGSWDWNLNGKNSTYHALYPRAWSVYDGEPDPELKIVCRQISPFIPHNYKESS 180
KE SGIGSWDWNL G NSTYHAL+PRAWSVYDGEPDP LKIVCRQISPFIPHNYKESS
Sbjct: 160 KESQVSGIGSWDWNLKGDNSTYHALFPRAWSVYDGEPDPALKIVCRQISPFIPHNYKESS 219
Query: 181 FPVA-----LSNSGETAANVSLLFTWTNSVGGDSESSGSHFNSRMPTKSGVHGVLLHHN- 234
PV+ L NSG+T+A+V+LLFTW NSVGG S SG H NSR K GVHGVLLHH
Sbjct: 220 LPVSVFTYTLYNSGKTSADVTLLFTWANSVGGLSGISGHHSNSRALMKDGVHGVLLHHKY 279
Query: 235 -TKDGHSPVTFAIAAQETADVKVSECPCFLISGNSQGITAKDMWNEIKERGSFDHLVSDN 293
T +G SPVTFAIAAQET V VSECPCF+ISG+S+G+TAKDMW+EIKE GSFD L S
Sbjct: 280 RTANGLSPVTFAIAAQETDGVHVSECPCFVISGDSKGVTAKDMWSEIKEHGSFDRLNSAE 339
Query: 294 TSMPSEPGSSIGAAVAASVSVPSNSTRTVTFSLAWACPEVNFPSGNTYHRRYTKFYGIHT 353
+PSEPGSSIGAA+AASV+VPS RTVTFSLAW CPE F G YHRRYTKFYG H
Sbjct: 340 MPLPSEPGSSIGAAIAASVAVPSGEVRTVTFSLAWDCPEAKFRGGKAYHRRYTKFYGTHG 399
Query: 354 DAAANLAHDAIL---------------------------------------------DGS 368
+AAAN+AHDAIL DGS
Sbjct: 400 EAAANIAHDAILEHRHWESQIEAWQRPVLEDKRLPEWYPVTLFNELYFLNSGGTVWTDGS 459
Query: 369 LPLRSLATSGERKFSINCSGSNIGTPTAEP-QNNISVGLSGKMISVLEEIQTPVPSNSAF 427
P+ SL + ERKFS++ S + + P QN+ ++ + G+M S LE++ TP+ +NSAF
Sbjct: 460 PPVHSLRSIIERKFSLDKSSLGLKSIIDAPEQNDTAIDILGRMTSTLEQVHTPIAANSAF 519
Query: 428 GSSLLQKGEENIGQFLYLEGIEYHMWNTCDVHFYSSFALIMLFPKLELSIQRDFAAAVMM 487
G++LLQ+GEENIGQFLYLEGIEY MWNT DVHFYSSFAL+MLFPKL+LSIQRDFAAAVM+
Sbjct: 520 GTNLLQEGEENIGQFLYLEGIEYQMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMI 579
Query: 488 HDPERVRMLDDGNWAQRKALGAVPHDLGLNNPWIEVNAYNFHNTNRWKDLNPKFVLQIYR 547
HDP ++R+L DG W QRK LGAVPHD+GL++PW EVNAYN +NT+RWKDLNPKFVLQ+YR
Sbjct: 580 HDPSKMRLLCDGRWVQRKVLGAVPHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYR 639
Query: 548 DVIATGDKSFAQAVWPAVYTAMAYMDQFDKDNDGMIENEGFPDQTYDVWSVKGVSAYSGG 607
DV+ATGDK FA+AVWPAVY AMAYM+QFDKD DGMIEN+GFPDQTYD WSV GVSAYSGG
Sbjct: 640 DVVATGDKKFAEAVWPAVYVAMAYMEQFDKDGDGMIENDGFPDQTYDTWSVYGVSAYSGG 699
Query: 608 LWVAALQAASAMAREVGDEVSEEHFWLKFQKAKAVYGQLWNGEYFNYDNSGGISSSSIQA 667
LWVAALQAAS MAREVGD+ SE +FW KFQKAKAVY +LWNG YFNYDNSG SSSSIQA
Sbjct: 700 LWVAALQAASGMAREVGDKGSEVYFWQKFQKAKAVYAKLWNGSYFNYDNSGQASSSSIQA 759
Query: 668 DQLAGQWYARACQLLPIVDEDKAQSALGKVYNFNVLKTNNGKRGAVNGMRPDGTVDMAGM 727
DQLAGQWYARAC LLPIVDEDKA+SAL K+YN+NVLK +G+RGAVNGM P+G VDM+ +
Sbjct: 760 DQLAGQWYARACGLLPIVDEDKARSALEKIYNYNVLKFEDGRRGAVNGMLPNGKVDMSTL 819
Query: 728 QSREIWSGVTYAVAASMIHEGLEEMGFKTASGVYEASWSQEGLGYAFQTPEAWDTDDKYR 787
QSREIWSGVTYAVAASMI E +MGF TA G+YEA WS+EGLGY+FQTPEAW T +YR
Sbjct: 820 QSREIWSGVTYAVAASMIQEDAIDMGFHTAEGIYEAGWSKEGLGYSFQTPEAWTTSGEYR 879
Query: 788 CLGYMRPLAIWAMQWALFPPKLFKEETSELLKEDPPLLFRNIDEFPRVAKLLKLPKEEDT 847
L YMRPLAIW+MQWAL P LFK+ET L+ D +L R+ F +VA+LLKLP EE +
Sbjct: 880 SLAYMRPLAIWSMQWALTKPPLFKQETE--LEVDEVILLRHKAGFSKVAQLLKLPPEESS 937
Query: 848 RSCLQFFYDYAFK 860
RS LQ +DY K
Sbjct: 938 RSILQTVFDYTCK 950