BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000108.1_g3380.1
(952 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010246138.1 PREDICTED: non-lysosomal glucosylceramidase isofo... 1418 0.0
XP_009341355.1 PREDICTED: non-lysosomal glucosylceramidase-like ... 1415 0.0
XP_009341356.1 PREDICTED: non-lysosomal glucosylceramidase-like ... 1411 0.0
>XP_010246138.1 PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Nelumbo
nucifera] XP_010246139.1 PREDICTED: non-lysosomal
glucosylceramidase isoform X1 [Nelumbo nucifera]
Length = 949
Score = 1418 bits (3670), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 679/953 (71%), Positives = 788/953 (82%), Gaps = 9/953 (0%)
Query: 1 MTKNGFYEGEEGPKSDVLVDKVEVDPGRPASLTWERRMDSDETVPLSFSPKLREVIHMIP 60
M +NGF EGE P S+ VDKV+VDP +PA LTW+R+++S V F+ ++E+IH+ P
Sbjct: 1 MLENGFNEGERDP-SNCFVDKVKVDPAQPALLTWQRKVNSQANVLSEFNLTIQEIIHLAP 59
Query: 61 LGYRLLRHVVAEKSKGQVAIIDPFHKRQITGCHGVPLGGIGAGSIGRSYRGDFQRFQLFP 120
+G+R+ R++ E SKG+ I+DPF KR +T CHGVPLGGIGAGSIGRSYRG+FQR+QLFP
Sbjct: 60 MGFRIWRYLKQEASKGRAIIMDPFKKRFVTSCHGVPLGGIGAGSIGRSYRGEFQRWQLFP 119
Query: 121 TICEDKPVLANQFSVFVSRSNGKRYDTVLSRGTQEFLQDDTTSGIGSWGWNFNGNNSTYH 180
ICE+KPVLANQFS FVSR NGK + TVL E + SGIGSW WN NG +STYH
Sbjct: 120 GICEEKPVLANQFSAFVSRENGKSFSTVLCPKNPELQMESNISGIGSWDWNLNGQSSTYH 179
Query: 181 ALFPRAWSVYKGEPDPELEIVCRQISPFIPHNYKESSLPVAVFTYTLSNSGDSAADVNLL 240
ALFPR+W+VY GEPDPEL IVCRQISPFIPHNYKESS PVAVFT+TL NSG++AAD+ LL
Sbjct: 180 ALFPRSWTVYDGEPDPELRIVCRQISPFIPHNYKESSFPVAVFTFTLFNSGETAADITLL 239
Query: 241 FTWANSVGGDSECSGGHVNSRMPTKDGVHGVLLHHNTGNGHAPVTFAIAAQETSDVKVSE 300
FTW NSVGGDS SG H NS+M K+GVHGVLLHH T +G PVT+AIAA+E+SD+ VSE
Sbjct: 240 FTWTNSVGGDSGFSGYHFNSKMKVKNGVHGVLLHHKTADGQPPVTYAIAAEESSDIHVSE 299
Query: 301 CPCFLISGNSQGVTAKDMWQEIKEHGSFDHLASVETSMPSNQGSSIGAAVAASLSVPSKS 360
CPCF+ISGNSQG+TAKDMW EIKEHGSFDHL S ETS S GSSIGAA+AASL++PS S
Sbjct: 300 CPCFIISGNSQGITAKDMWHEIKEHGSFDHLGSCETSTISEPGSSIGAAIAASLTLPSNS 359
Query: 361 TRTVTFSLAWACPEVNFSSGNTYHRRYTKFYGTHTDAAANLAHDAIFEHGQWESQIEAWQ 420
+RTVTFSLAW+CPEV FSSGN YHRRYTKFYGT+ DAA N+A+DAI EH WESQIEAWQ
Sbjct: 360 SRTVTFSLAWSCPEVKFSSGNIYHRRYTKFYGTNCDAAENIAYDAIHEHCYWESQIEAWQ 419
Query: 421 QPILEDKRLPEWYPVTLFNELYYLNAGGTIWTDGALPMRSLATIGERKFSIDNSGSDI-N 479
+PIL D+ LPEWYP+TLFNELYYLNAGGTIWTDGA P+++L++IGE KFS+D SD N
Sbjct: 420 RPILNDQSLPEWYPITLFNELYYLNAGGTIWTDGAQPVQNLSSIGEIKFSLDRYRSDSKN 479
Query: 480 IPSTDPQNNIAVGLLGRMTSVLEQIQTPVASNSAFGTSLLQKGEENIGQFLYLEGNEYLM 539
QN+ +V +L RM S+LEQI TPV S+SA GT LLQK EENIGQFLYLEG EY M
Sbjct: 480 TVDNAHQNDTSVDILERMASILEQIHTPVKSSSALGTFLLQKDEENIGQFLYLEGIEYHM 539
Query: 540 WNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVMMHDPESVKSFDDGNWAQRKALGAVPH 599
WNTYDVHFYSS AL+MLFPKLELSIQRDFAAAVMMHDP ++ +G W RK LGAVPH
Sbjct: 540 WNTYDVHFYSSCALVMLFPKLELSIQRDFAAAVMMHDPSRMQVLSNGRWVPRKVLGAVPH 599
Query: 600 DLGLKNPWFEVNAYNFYNTDRWKDLNPKFVLQVYRDVIATGDKSFTQAVWPAVYMAMAYM 659
DLG+ +PWFEVNAYN +NTDRWKDLNPKFVLQVYRDV+ TGDK F ++VW +VY+AMAYM
Sbjct: 600 DLGMHDPWFEVNAYNLHNTDRWKDLNPKFVLQVYRDVVVTGDKLFAKSVWRSVYIAMAYM 659
Query: 660 DQFDKDRDGMIENEGFPDQTYDVWSVKGISAYSGGLWVAALQAASAMAREVGDKVSEDHF 719
DQFDKD DGMIENEGFPDQTYDVWSV G+SAYSGGLWVAALQAAS MAREVGDK SE +F
Sbjct: 660 DQFDKDGDGMIENEGFPDQTYDVWSVTGVSAYSGGLWVAALQAASFMAREVGDKTSEGYF 719
Query: 720 WLKFQKAKAVYEQLWNGEYFNYDNSCGGSSSSIQADQLAGQWYARACQLLPIVDEDKAQS 779
W KFQ+AK+VYE+LWNG YFNYDN+ SSSSIQADQLAGQWYARAC L PIVD++K +S
Sbjct: 720 WDKFQRAKSVYEKLWNGSYFNYDNNNDSSSSSIQADQLAGQWYARACGLQPIVDKEKVKS 779
Query: 780 ALDKVYNFNVLKMKNGKRGAVNGMRPDGTVDMTALQSREIWAGVTYAVAASMIQEGLEDM 839
AL+KVYNFNVL++K G+RGAVNGM PDG VDM+A+QSREIW+GVTY++AASMI EG+ DM
Sbjct: 780 ALEKVYNFNVLRVKGGRRGAVNGMLPDGRVDMSAMQSREIWSGVTYSLAASMIHEGMIDM 839
Query: 840 GFKTASGVYEASWSQEGLGYAFQTPEAWDNEDKYRSLTYMRPLAIWAMQWALSPPKLFKE 899
F TA GVYE +WS EGLGY+FQTPE W +D+YRSL YMRPLAIWAMQWALSPPKLF +
Sbjct: 840 AFNTAYGVYETAWSHEGLGYSFQTPEGWTTDDQYRSLAYMRPLAIWAMQWALSPPKLFIK 899
Query: 900 ER-TETSELPKEAPPLLFRNIDGFSRVAKLLKLPKEEDSRSYLQMFYDKAVRR 951
ER +ET E PK F + GFS+VA+LLKLP++E S+S+LQ+ YD RR
Sbjct: 900 ERGSETEEYPK------FMHHSGFSKVAQLLKLPEDEASKSFLQVVYDCTCRR 946
>XP_009341355.1 PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Pyrus
x bretschneideri]
Length = 966
Score = 1415 bits (3664), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 678/953 (71%), Positives = 781/953 (81%), Gaps = 11/953 (1%)
Query: 1 MTKNGFYEGEEGPKSDVLVDKVEVDPGRPASLTWERRMDSDETVPLSFSPKLREVIHMIP 60
+ +NGF E + SD ++KV DPG+PASLTW+R+++S + PL F+ L+E+IH+ P
Sbjct: 20 ILENGFAE-RDYEHSDSSLEKV--DPGKPASLTWQRKLNSKGSAPLPFTLSLKEIIHLAP 76
Query: 61 LGYRLLRHVVAEKSKGQVAIIDPFHKRQITGCHGVPLGGIGAGSIGRSYRGDFQRFQLFP 120
+G RL RH+ E + + IDPF KR +T HGVPLGGIGAGSIGRSY GDFQR+QLFP
Sbjct: 77 IGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPLGGIGAGSIGRSYSGDFQRWQLFP 136
Query: 121 TICEDKPVLANQFSVFVSRSNGKRYDTVLSRGTQEFLQDDTTSGIGSWGWNFNGNNSTYH 180
CE+KPVLA+QFSVFVSR+NG++Y TVL + + L++ SGIGSW WN G+NSTYH
Sbjct: 137 GRCEEKPVLADQFSVFVSRTNGEKYSTVLCPRSPDDLKESQVSGIGSWDWNLKGDNSTYH 196
Query: 181 ALFPRAWSVYKGEPDPELEIVCRQISPFIPHNYKESSLPVAVFTYTLSNSGDSAADVNLL 240
ALFPRAWSVY GEPDP L+IVCRQISPFIPHNYKESSLPV+VFTYTL NSG ++ADV LL
Sbjct: 197 ALFPRAWSVYDGEPDPALKIVCRQISPFIPHNYKESSLPVSVFTYTLYNSGKTSADVTLL 256
Query: 241 FTWANSVGGDSECSGGHVNSRMPTKDGVHGVLLHHNTGNGHAPVTFAIAAQETSDVKVSE 300
FTWANSVGG S SG H NSR KDGVHGVLLHH T NG +PVTFAIAAQET V VSE
Sbjct: 257 FTWANSVGGLSGISGHHSNSRALMKDGVHGVLLHHKTANGLSPVTFAIAAQETDGVHVSE 316
Query: 301 CPCFLISGNSQGVTAKDMWQEIKEHGSFDHLASVETSMPSNQGSSIGAAVAASLSVPSKS 360
CPCF+ISG+S+GVTAKDMW EIKEHGSFD L S E +PS GSSIGAA+AAS++VPS
Sbjct: 317 CPCFVISGDSKGVTAKDMWSEIKEHGSFDRLNSAEMPLPSEPGSSIGAAIAASVAVPSGE 376
Query: 361 TRTVTFSLAWACPEVNFSSGNTYHRRYTKFYGTHTDAAANLAHDAIFEHGQWESQIEAWQ 420
RTVTFSLAW CPE F G YHRRYTKFYGTH +AAAN+AHDAI EH WESQIEAWQ
Sbjct: 377 VRTVTFSLAWDCPEAKFRGGKAYHRRYTKFYGTHGEAAANIAHDAILEHRHWESQIEAWQ 436
Query: 421 QPILEDKRLPEWYPVTLFNELYYLNAGGTIWTDGALPMRSLATIGERKFSIDNSGSDI-N 479
+P+LEDKRLPEWYPVTLFNELY+LN+GGT+WTDG+ P+ SL +I ERKFS+D S + +
Sbjct: 437 RPVLEDKRLPEWYPVTLFNELYFLNSGGTVWTDGSPPVHSLRSIIERKFSLDKSSLGLKS 496
Query: 480 IPSTDPQNNIAVGLLGRMTSVLEQIQTPVASNSAFGTSLLQKGEENIGQFLYLEGNEYLM 539
I QN+ A+ +LGRMTS LEQ+ TP+A+NSAFGT+LLQ+GEENIGQFLYLEG EY M
Sbjct: 497 IIDAPEQNDTAIDILGRMTSTLEQVHTPIAANSAFGTNLLQEGEENIGQFLYLEGIEYQM 556
Query: 540 WNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVMMHDPESVKSFDDGNWAQRKALGAVPH 599
WNTYDVHFYSSFAL+MLFPKL+LSIQRDFAAAVM+HDP ++ DG W QRK LGAVPH
Sbjct: 557 WNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMIHDPSKMRLLCDGRWVQRKVLGAVPH 616
Query: 600 DLGLKNPWFEVNAYNFYNTDRWKDLNPKFVLQVYRDVIATGDKSFTQAVWPAVYMAMAYM 659
D+GL +PWFEVNAYN YNTDRWKDLNPKFVLQVYRDV+ATGDK F +AVWPAVY+AMAYM
Sbjct: 617 DIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDVVATGDKKFAEAVWPAVYVAMAYM 676
Query: 660 DQFDKDRDGMIENEGFPDQTYDVWSVKGISAYSGGLWVAALQAASAMAREVGDKVSEDHF 719
+QFDKD DGMIEN+GFPDQTYD WSV G+SAYSGGLWVAALQAAS MAREVGDK SE +F
Sbjct: 677 EQFDKDGDGMIENDGFPDQTYDTWSVYGVSAYSGGLWVAALQAASGMAREVGDKGSEVYF 736
Query: 720 WLKFQKAKAVYEQLWNGEYFNYDNSCGGSSSSIQADQLAGQWYARACQLLPIVDEDKAQS 779
W KFQKAKAVY +LWNG YFNYDNS SSSSIQADQLAGQWYARAC LLPIVDEDKA+S
Sbjct: 737 WQKFQKAKAVYAKLWNGSYFNYDNSGQASSSSIQADQLAGQWYARACGLLPIVDEDKARS 796
Query: 780 ALDKVYNFNVLKMKNGKRGAVNGMRPDGTVDMTALQSREIWAGVTYAVAASMIQEGLEDM 839
AL+K+YN+NVLK ++G+RGAVNGM P+G VDM+ LQSREIW+GVTYAVAASMIQE DM
Sbjct: 797 ALEKIYNYNVLKFEDGRRGAVNGMLPNGKVDMSTLQSREIWSGVTYAVAASMIQEDAIDM 856
Query: 840 GFKTASGVYEASWSQEGLGYAFQTPEAWDNEDKYRSLTYMRPLAIWAMQWALSPPKLFKE 899
GF TA G+YEA WS+EGLGY+FQTPEAW +YRSL YMRPLAIW+MQWAL+ P LFK+
Sbjct: 857 GFHTAEGIYEAGWSKEGLGYSFQTPEAWTTSGEYRSLAYMRPLAIWSMQWALTKPPLFKQ 916
Query: 900 E-RTETSELPKEAPPLLFRNIDGFSRVAKLLKLPKEEDSRSYLQMFYDKAVRR 951
E E E+ +L R+ GFS+VA+LLKLP EE SRS LQ +D +R
Sbjct: 917 ETELEVDEV------ILLRHKAGFSKVAQLLKLPPEESSRSILQTVFDYTCKR 963
>XP_009341356.1 PREDICTED: non-lysosomal glucosylceramidase-like isoform X3 [Pyrus
x bretschneideri]
Length = 954
Score = 1411 bits (3652), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 678/955 (70%), Positives = 781/955 (81%), Gaps = 13/955 (1%)
Query: 1 MTKNGFYEGEEGPKSDVLVDKVEVDPGRPASLTWERRMDSDETVPLSFSPKLREVIHMIP 60
+ +NGF E + SD ++KV DPG+PASLTW+R+++S + PL F+ L+E+IH+ P
Sbjct: 6 ILENGFAE-RDYEHSDSSLEKV--DPGKPASLTWQRKLNSKGSAPLPFTLSLKEIIHLAP 62
Query: 61 LGYRLLRHVVAEKSKGQVAIIDPFHKRQITGCHGVPLGGIGAGSIGRSYRGDFQRFQLFP 120
+G RL RH+ E + + IDPF KR +T HGVPLGGIGAGSIGRSY GDFQR+QLFP
Sbjct: 63 IGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPLGGIGAGSIGRSYSGDFQRWQLFP 122
Query: 121 TICEDKPVLANQFSVFVSRSNGKRYDTVLSRGTQEFLQDDTTSGIGSWGWNFNGNNSTYH 180
CE+KPVLA+QFSVFVSR+NG++Y TVL + + L++ SGIGSW WN G+NSTYH
Sbjct: 123 GRCEEKPVLADQFSVFVSRTNGEKYSTVLCPRSPDDLKESQVSGIGSWDWNLKGDNSTYH 182
Query: 181 ALFPRAWSVYKGEPDPELEIVCRQISPFIPHNYKESSLPVAVFTYTLSNSGDSAADVNLL 240
ALFPRAWSVY GEPDP L+IVCRQISPFIPHNYKESSLPV+VFTYTL NSG ++ADV LL
Sbjct: 183 ALFPRAWSVYDGEPDPALKIVCRQISPFIPHNYKESSLPVSVFTYTLYNSGKTSADVTLL 242
Query: 241 FTWANSVGGDSECSGGHVNSRMPTKDGVHGVLLHHN--TGNGHAPVTFAIAAQETSDVKV 298
FTWANSVGG S SG H NSR KDGVHGVLLHH T NG +PVTFAIAAQET V V
Sbjct: 243 FTWANSVGGLSGISGHHSNSRALMKDGVHGVLLHHKYRTANGLSPVTFAIAAQETDGVHV 302
Query: 299 SECPCFLISGNSQGVTAKDMWQEIKEHGSFDHLASVETSMPSNQGSSIGAAVAASLSVPS 358
SECPCF+ISG+S+GVTAKDMW EIKEHGSFD L S E +PS GSSIGAA+AAS++VPS
Sbjct: 303 SECPCFVISGDSKGVTAKDMWSEIKEHGSFDRLNSAEMPLPSEPGSSIGAAIAASVAVPS 362
Query: 359 KSTRTVTFSLAWACPEVNFSSGNTYHRRYTKFYGTHTDAAANLAHDAIFEHGQWESQIEA 418
RTVTFSLAW CPE F G YHRRYTKFYGTH +AAAN+AHDAI EH WESQIEA
Sbjct: 363 GEVRTVTFSLAWDCPEAKFRGGKAYHRRYTKFYGTHGEAAANIAHDAILEHRHWESQIEA 422
Query: 419 WQQPILEDKRLPEWYPVTLFNELYYLNAGGTIWTDGALPMRSLATIGERKFSIDNSGSDI 478
WQ+P+LEDKRLPEWYPVTLFNELY+LN+GGT+WTDG+ P+ SL +I ERKFS+D S +
Sbjct: 423 WQRPVLEDKRLPEWYPVTLFNELYFLNSGGTVWTDGSPPVHSLRSIIERKFSLDKSSLGL 482
Query: 479 -NIPSTDPQNNIAVGLLGRMTSVLEQIQTPVASNSAFGTSLLQKGEENIGQFLYLEGNEY 537
+I QN+ A+ +LGRMTS LEQ+ TP+A+NSAFGT+LLQ+GEENIGQFLYLEG EY
Sbjct: 483 KSIIDAPEQNDTAIDILGRMTSTLEQVHTPIAANSAFGTNLLQEGEENIGQFLYLEGIEY 542
Query: 538 LMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVMMHDPESVKSFDDGNWAQRKALGAV 597
MWNTYDVHFYSSFAL+MLFPKL+LSIQRDFAAAVM+HDP ++ DG W QRK LGAV
Sbjct: 543 QMWNTYDVHFYSSFALVMLFPKLQLSIQRDFAAAVMIHDPSKMRLLCDGRWVQRKVLGAV 602
Query: 598 PHDLGLKNPWFEVNAYNFYNTDRWKDLNPKFVLQVYRDVIATGDKSFTQAVWPAVYMAMA 657
PHD+GL +PWFEVNAYN YNTDRWKDLNPKFVLQVYRDV+ATGDK F +AVWPAVY+AMA
Sbjct: 603 PHDIGLHDPWFEVNAYNLYNTDRWKDLNPKFVLQVYRDVVATGDKKFAEAVWPAVYVAMA 662
Query: 658 YMDQFDKDRDGMIENEGFPDQTYDVWSVKGISAYSGGLWVAALQAASAMAREVGDKVSED 717
YM+QFDKD DGMIEN+GFPDQTYD WSV G+SAYSGGLWVAALQAAS MAREVGDK SE
Sbjct: 663 YMEQFDKDGDGMIENDGFPDQTYDTWSVYGVSAYSGGLWVAALQAASGMAREVGDKGSEV 722
Query: 718 HFWLKFQKAKAVYEQLWNGEYFNYDNSCGGSSSSIQADQLAGQWYARACQLLPIVDEDKA 777
+FW KFQKAKAVY +LWNG YFNYDNS SSSSIQADQLAGQWYARAC LLPIVDEDKA
Sbjct: 723 YFWQKFQKAKAVYAKLWNGSYFNYDNSGQASSSSIQADQLAGQWYARACGLLPIVDEDKA 782
Query: 778 QSALDKVYNFNVLKMKNGKRGAVNGMRPDGTVDMTALQSREIWAGVTYAVAASMIQEGLE 837
+SAL+K+YN+NVLK ++G+RGAVNGM P+G VDM+ LQSREIW+GVTYAVAASMIQE
Sbjct: 783 RSALEKIYNYNVLKFEDGRRGAVNGMLPNGKVDMSTLQSREIWSGVTYAVAASMIQEDAI 842
Query: 838 DMGFKTASGVYEASWSQEGLGYAFQTPEAWDNEDKYRSLTYMRPLAIWAMQWALSPPKLF 897
DMGF TA G+YEA WS+EGLGY+FQTPEAW +YRSL YMRPLAIW+MQWAL+ P LF
Sbjct: 843 DMGFHTAEGIYEAGWSKEGLGYSFQTPEAWTTSGEYRSLAYMRPLAIWSMQWALTKPPLF 902
Query: 898 KEE-RTETSELPKEAPPLLFRNIDGFSRVAKLLKLPKEEDSRSYLQMFYDKAVRR 951
K+E E E+ +L R+ GFS+VA+LLKLP EE SRS LQ +D +R
Sbjct: 903 KQETELEVDEV------ILLRHKAGFSKVAQLLKLPPEESSRSILQTVFDYTCKR 951