BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000117.1_g0960.1
(670 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010257856.1 PREDICTED: serine protease SPPA, chloroplastic [N... 892 0.0
XP_009337777.1 PREDICTED: serine protease SPPA, chloroplastic-li... 874 0.0
XP_002268894.1 PREDICTED: serine protease SPPA, chloroplastic [V... 873 0.0
>XP_010257856.1 PREDICTED: serine protease SPPA, chloroplastic [Nelumbo nucifera]
Length = 704
Score = 892 bits (2305), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 447/675 (66%), Positives = 536/675 (79%), Gaps = 49/675 (7%)
Query: 3 SEKKFESMNSESIGQVVTEKEEDQAE---TKAKT-LEENRILKGNNGDEYPSGEFEFKKF 58
S + FES SE+ + + K+E+ + TKA T L++N L+G+ DEYPSGEFEFK+
Sbjct: 72 SVRAFESA-SETKNEEIPRKDENISSSDSTKADTKLDDNGSLRGSK-DEYPSGEFEFKEI 129
Query: 59 TSWKKFIVKLRMLVAYPWERVQNGSVLSIKLRGQVRFIIYFVFSLTLMYLLNVEAHNSFE 118
+ WK F VKLRML+A+PWERV+ GSVLS+KLRGQ
Sbjct: 130 SGWKNFAVKLRMLIAFPWERVRKGSVLSMKLRGQ-------------------------- 163
Query: 119 FDPCLICEHNSIQISDQLKSRFSSKLSLPRICENFNKAAYDPRVSGIYLQIESLKCGWAK 178
ISDQLKSRFSS LSLP+ICENF KAAYDPR+SGIYL IE L CGW K
Sbjct: 164 -------------ISDQLKSRFSSGLSLPQICENFIKAAYDPRISGIYLHIEPLSCGWGK 210
Query: 179 LEEIRRHIVDFRKSGKFIVAYVPICHEKDYYIGSACGELYCPPSAYFSLYGLTVQASFFG 238
+EEIRRHI++FRKSGKFIV YVP C EK+YY+G AC ELY PPSAYF+LYGL VQA+F G
Sbjct: 211 VEEIRRHILNFRKSGKFIVGYVPACGEKEYYLGCACEELYAPPSAYFALYGLAVQATFLG 270
Query: 239 GVFEKVGVEPQVERIGKYKSYGDRRTRKSMSEENREMMTAVLDSRYGNWLDTIASTLGKR 298
G+FEKVG+EPQV+RIGKYKS GD+ TRK+MS+EN EM+TA+LD+ YGNWLD ++S+ GK+
Sbjct: 271 GIFEKVGIEPQVQRIGKYKSAGDQLTRKNMSQENCEMLTALLDNIYGNWLDKVSSSKGKK 330
Query: 299 REEIEDFVNEGAYQVERLKEDGWITDIQYDDEIIQMLKERLGQDKDKNIRMVDYKKYSRV 358
REEIE F+NEG Y+V+RLKE+GWITDI+YDDE+I MLKERLG K+K + MVDY+KYSRV
Sbjct: 331 REEIEAFINEGVYEVQRLKEEGWITDIRYDDEVISMLKERLGVKKEKKLSMVDYRKYSRV 390
Query: 359 KNWTLGLSGGKKQIAVIRASGSITRVRN--SIFSSGIVSEQFIKKIRRVRESKKYKAVIL 416
+NWTLGLSGGK QIAVIRASGSI RVR+ S+ SSGI++EQFI+K+R VRESK+YKAVI+
Sbjct: 391 RNWTLGLSGGKDQIAVIRASGSIIRVRSPLSLPSSGIIAEQFIEKVRSVRESKRYKAVII 450
Query: 417 RIDSPGGEALASDLMWREIKLLAASKPVVASMSDVAASGGYYMAMAAGCIVAENLTLTGS 476
RIDSPGG+ALASDLMWREI+LLAASKPV+ASMSDVAASGGYYMAMA G IVAENLTLTGS
Sbjct: 451 RIDSPGGDALASDLMWREIRLLAASKPVIASMSDVAASGGYYMAMATGTIVAENLTLTGS 510
Query: 477 IGVVIENYNAGKLHEKIGFNKEIISRGKYADFRAT-NRSFRPDEAELFTKTAQTIYKRFR 535
IGVV +N G L+EKIGFNKEIISRGKYA+ A R FRPDEA+LF K+AQ YK+FR
Sbjct: 511 IGVVTGKFNLGNLYEKIGFNKEIISRGKYAELNAAEQRPFRPDEAKLFAKSAQNAYKQFR 570
Query: 536 DKAALSRSMTIDEMEEFAQGRVWSGNDAACRGLIDALGGFSRAVAIAKQKAGIAQDKQVK 595
DKAA SRSM +D+ME AQGRVW+GNDAA RGL+DA+GG SRAVAIAKQKA I QD+QV
Sbjct: 571 DKAAFSRSMDVDQMENVAQGRVWTGNDAASRGLVDAIGGLSRAVAIAKQKANIPQDRQVS 630
Query: 596 LVELSKPPSPTLLGLLKGTLIKFIGLNGTMRELLQNITTSEGVQAKMEGLGFEGLEGASY 655
LVELS+ PSPTL +L G IG++ T++ELLQ++T S+GVQA+M+G+ FE LEG S
Sbjct: 631 LVELSR-PSPTLPEILSGIGNSIIGVDRTVKELLQDLTFSDGVQARMDGIMFERLEGTSN 689
Query: 656 VNPIFAVIEDYISSI 670
NPIF +++DY+SS+
Sbjct: 690 TNPIFTLMKDYLSSL 704
>XP_009337777.1 PREDICTED: serine protease SPPA, chloroplastic-like [Pyrus x
bretschneideri]
Length = 678
Score = 874 bits (2259), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 429/650 (66%), Positives = 515/650 (79%), Gaps = 44/650 (6%)
Query: 25 DQAETKAKTLEENRILKGNNGD-EYPSGEFEFKKFTSWKKFIVKLRMLVAYPWERVQNGS 83
D E ++K + + K D EYPSGEF+F+K ++WK F+VKLRML+A+PW+RV+ GS
Sbjct: 69 DTKEDESKETDGEIVNKSKTADKEYPSGEFQFEKASAWKSFVVKLRMLIAFPWQRVKKGS 128
Query: 84 VLSIKLRGQVRFIIYFVFSLTLMYLLNVEAHNSFEFDPCLICEHNSIQISDQLKSRFSSK 143
VL+IKLRGQV SDQLKSRFSS
Sbjct: 129 VLTIKLRGQV---------------------------------------SDQLKSRFSSG 149
Query: 144 LSLPRICENFNKAAYDPRVSGIYLQIESLKCGWAKLEEIRRHIVDFRKSGKFIVAYVPIC 203
LSLP+ICEN KAAYDPR+SG+YLQIESL CGW K+EEIRRHI+DF+KSGKF+VAYVP C
Sbjct: 150 LSLPQICENLIKAAYDPRISGVYLQIESLNCGWGKVEEIRRHILDFKKSGKFVVAYVPAC 209
Query: 204 HEKDYYIGSACGELYCPPSAYFSLYGLTVQASFFGGVFEKVGVEPQVERIGKYKSYGDRR 263
EK+YY+ SAC E+Y PPSAYFSL+GLTVQASF GV EKVG+EPQVERIGKYKS GD+
Sbjct: 210 REKEYYLASACQEIYAPPSAYFSLFGLTVQASFIRGVLEKVGIEPQVERIGKYKSAGDQL 269
Query: 264 TRKSMSEENREMMTAVLDSRYGNWLDTIASTLGKRREEIEDFVNEGAYQVERLKEDGWIT 323
RK+MS+EN EM+TA+LD+ YGNWLD I+ST GK+RE+IE+F+NEG YQVE+LKE+GWIT
Sbjct: 270 ARKTMSKENCEMLTALLDNIYGNWLDVISSTRGKKREDIENFINEGVYQVEKLKEEGWIT 329
Query: 324 DIQYDDEIIQMLKERLGQDKDKNIRMVDYKKYSRVKNWTLGLSGGKKQIAVIRASGSITR 383
+IQYDDE+I MLKERLG K+K + MVDYKKYS+V+ WT+GLSGGK +IA+IRASGSITR
Sbjct: 330 NIQYDDEVISMLKERLGVQKEKTLPMVDYKKYSKVRQWTVGLSGGKDKIAIIRASGSITR 389
Query: 384 VRN--SIFSSGIVSEQFIKKIRRVRESKKYKAVILRIDSPGGEALASDLMWREIKLLAAS 441
VR S+ S I+ EQFI+KIR VRESK+YKA I+RIDSPGG+ALASDLMWREI+LLA S
Sbjct: 390 VRGGLSLPGSSIIGEQFIEKIRSVRESKRYKAAIIRIDSPGGDALASDLMWREIRLLATS 449
Query: 442 KPVVASMSDVAASGGYYMAMAAGCIVAENLTLTGSIGVVIENYNAGKLHEKIGFNKEIIS 501
KPV+ASMSDVAASGGYYMAMAA IVAENLTLTGSIGVV +N GKL+EKIGFNKEIIS
Sbjct: 450 KPVIASMSDVAASGGYYMAMAADTIVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEIIS 509
Query: 502 RGKYAD-FRATNRSFRPDEAELFTKTAQTIYKRFRDKAALSRSMTIDEMEEFAQGRVWSG 560
RGKYA+ A R FRP+EAELF K+AQ YK+FRDKAA SRSMT+D+MEE AQGRVW+G
Sbjct: 510 RGKYAEVLAAEQRPFRPEEAELFAKSAQNAYKQFRDKAAFSRSMTVDKMEEVAQGRVWTG 569
Query: 561 NDAACRGLIDALGGFSRAVAIAKQKAGIAQDKQVKLVELSKPPSPTLLGLLKGTLIKFIG 620
NDA RGL+DA+GGFSRAVAIAK KA I QD+QV LVEL++ PSPTL LL G +G
Sbjct: 570 NDAVSRGLVDAIGGFSRAVAIAKLKANIPQDRQVALVELAR-PSPTLPELLSGIGATLVG 628
Query: 621 LNGTMRELLQNITTSEGVQAKMEGLGFEGLEGASYVNPIFAVIEDYISSI 670
++ TM+E+LQ +T S+GVQA+M+G+ F+ LEGAS NPIF++++DY S+
Sbjct: 629 VDRTMKEVLQELTFSDGVQARMDGIMFQRLEGASQANPIFSLLKDYFGSL 678
>XP_002268894.1 PREDICTED: serine protease SPPA, chloroplastic [Vitis vinifera]
Length = 686
Score = 873 bits (2256), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 436/671 (64%), Positives = 525/671 (78%), Gaps = 48/671 (7%)
Query: 3 SEKKFESMNSESIGQVVTEKEEDQAETKAKTLEENRILKGNNGDEYPSGEFEFKKFTSWK 62
S + F+S +SE+ VV+E +A K ++ + + +EYP+G+FEFK+ + W
Sbjct: 61 SLRAFDS-SSETKSDVVSE----EAGEKDYKDDDGALSSTSLAEEYPTGDFEFKEMSGWM 115
Query: 63 KFIVKLRMLVAYPWERVQNGSVLSIKLRGQVRFIIYFVFSLTLMYLLNVEAHNSFEFDPC 122
F+VKLRML+A+PWERV+ GSV ++KLRGQ
Sbjct: 116 SFVVKLRMLIAFPWERVRKGSVFTMKLRGQ------------------------------ 145
Query: 123 LICEHNSIQISDQLKSRFSSKLSLPRICENFNKAAYDPRVSGIYLQIESLKCGWAKLEEI 182
ISDQLKSRFSS LSLP+ICENF KAAYDPR+SGIYL IE L CGW K+EEI
Sbjct: 146 ---------ISDQLKSRFSSGLSLPQICENFIKAAYDPRISGIYLHIEPLSCGWGKVEEI 196
Query: 183 RRHIVDFRKSGKFIVAYVPICHEKDYYIGSACGELYCPPSAYFSLYGLTVQASFFGGVFE 242
RRHI+DF+KSGKFIVAY P C EK+YY+GSAC ELY PPSAYFSLYGLTVQASF GGVFE
Sbjct: 197 RRHILDFKKSGKFIVAYAPACGEKEYYLGSACDELYAPPSAYFSLYGLTVQASFLGGVFE 256
Query: 243 KVGVEPQVERIGKYKSYGDRRTRKSMSEENREMMTAVLDSRYGNWLDTIASTLGKRREEI 302
KVG+EPQV+RIGKYKS GD+ TRK+MSEEN EM+TA+LD+ YGNWLD I+S GK+RE+
Sbjct: 257 KVGIEPQVQRIGKYKSAGDQLTRKTMSEENCEMLTALLDNIYGNWLDKISSAKGKKREDT 316
Query: 303 EDFVNEGAYQVERLKEDGWITDIQYDDEIIQMLKERLGQDKDKNIRMVDYKKYSRVKNWT 362
E+F+NEG YQVE+LKE+GWIT+I YDDE+I +LKERLGQ KDKN+ MVDY+KYS+V+ WT
Sbjct: 317 ENFINEGVYQVEKLKEEGWITNINYDDEVISILKERLGQPKDKNLPMVDYRKYSKVRKWT 376
Query: 363 LGLSGGKKQIAVIRASGSITRVRN--SIFSSGIVSEQFIKKIRRVRESKKYKAVILRIDS 420
LGLSGGK QIAVIRASGSI+RVR+ SI SGI SEQFI+KIR VR+SK+YKAVI+RIDS
Sbjct: 377 LGLSGGKDQIAVIRASGSISRVRSPFSIPGSGITSEQFIEKIRSVRDSKRYKAVIIRIDS 436
Query: 421 PGGEALASDLMWREIKLLAASKPVVASMSDVAASGGYYMAMAAGCIVAENLTLTGSIGVV 480
PGG+ALASDLMWREI+LLAASKPV+ASMSDVAASGGYYMAM AG IVAENLTLTGSIGVV
Sbjct: 437 PGGDALASDLMWREIRLLAASKPVIASMSDVAASGGYYMAMGAGTIVAENLTLTGSIGVV 496
Query: 481 IENYNAGKLHEKIGFNKEIISRGKYADFRAT-NRSFRPDEAELFTKTAQTIYKRFRDKAA 539
+N G L+EKIGFNKEIISRG++A+ A R FRPDEAELF K+AQ YK+FRDKAA
Sbjct: 497 TGKFNLGTLYEKIGFNKEIISRGRFAELTAAEQRPFRPDEAELFAKSAQNAYKQFRDKAA 556
Query: 540 LSRSMTIDEMEEFAQGRVWSGNDAACRGLIDALGGFSRAVAIAKQKAGIAQDKQVKLVEL 599
SRSM +D+MEE AQGRVW+G DAA RGL+DA+GG SRAVAIAKQKA I QD+ V LVEL
Sbjct: 557 FSRSMAVDKMEENAQGRVWTGKDAASRGLVDAIGGLSRAVAIAKQKADIPQDRPVTLVEL 616
Query: 600 SKPPSPTLLGLLKGTLIKFIGLNGTMRELLQNITTSEGVQAKMEGLGFEGLEGASYVNPI 659
S+ PSPT+ +L G +G+ T++ELLQ++T S GVQA+M+G+ F+ LE AS NPI
Sbjct: 617 SR-PSPTVSEILTGIGSSIVGVERTLKELLQDLTFSNGVQARMDGILFQKLEEASDSNPI 675
Query: 660 FAVIEDYISSI 670
F +++DY+SS+
Sbjct: 676 FTLVKDYLSSL 686