BLAST Resut
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eca_sc000117.1_g0970.1
(697 letters)
Database: ./nr
95,329,361 sequences; 35,143,497,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010257856.1 PREDICTED: serine protease SPPA, chloroplastic [N... 1077 0.0
OAY59330.1 hypothetical protein MANES_01G024300 [Manihot esculenta] 1036 0.0
XP_002268894.1 PREDICTED: serine protease SPPA, chloroplastic [V... 1036 0.0
>XP_010257856.1 PREDICTED: serine protease SPPA, chloroplastic [Nelumbo nucifera]
Length = 704
Score = 1077 bits (2785), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 535/710 (75%), Positives = 610/710 (85%), Gaps = 19/710 (2%)
Query: 1 MSKLLCTSHLNVVNICCSSSSSILSRSPLPIQLSKTLSLS--DSQS-----SSLTKRRNL 53
MSKLLCTS LN ++ S S++IL +S P+QL + D Q+ SSL++RR +
Sbjct: 1 MSKLLCTSRLNGLH-SLSFSATIL-KSSFPVQLGHSAYRPHLDYQTYVSLPSSLSRRRVI 58
Query: 54 HLSFSNSNPTRYLQIRAFESSSSSETTNQNLPNKEEN-EAENEVRAS---DENGSLKVNN 109
S S R L +RAFES+S ET N+ +P K+EN + + +A D+NGSL+
Sbjct: 59 LQSLYGSIRPRDLSVRAFESAS--ETKNEEIPRKDENISSSDSTKADTKLDDNGSLR--G 114
Query: 110 NKDEYPSGEFEFKEFNLWKSFVVKFRMLFAYPWERVRKGSVLSMKLRGEISDQLKSRFSS 169
+KDEYPSGEFEFKE + WK+F VK RML A+PWERVRKGSVLSMKLRG+ISDQLKSRFSS
Sbjct: 115 SKDEYPSGEFEFKEISGWKNFAVKLRMLIAFPWERVRKGSVLSMKLRGQISDQLKSRFSS 174
Query: 170 GLSLPQICENFIKAAYDPRISGIYLQIEPLNCGWGKVEEIRRHILDFRKSGKFVVAYVPI 229
GLSLPQICENFIKAAYDPRISGIYL IEPL+CGWGKVEEIRRHIL+FRKSGKF+V YVP
Sbjct: 175 GLSLPQICENFIKAAYDPRISGIYLHIEPLSCGWGKVEEIRRHILNFRKSGKFIVGYVPA 234
Query: 230 CQEKEYYIASACGELYCPPSAYFALYGLTVQSSFLGGIFEKVGIEPQVERIGKYKSAGDQ 289
C EKEYY+ AC ELY PPSAYFALYGL VQ++FLGGIFEKVGIEPQV+RIGKYKSAGDQ
Sbjct: 235 CGEKEYYLGCACEELYAPPSAYFALYGLAVQATFLGGIFEKVGIEPQVQRIGKYKSAGDQ 294
Query: 290 LARKSMSEENCEMLTALLDNIYGNWLDKISSTLGKKREEIENFVNEGVFQVERLKEDGWI 349
L RK+MS+ENCEMLTALLDNIYGNWLDK+SS+ GKKREEIE F+NEGV++V+RLKE+GWI
Sbjct: 295 LTRKNMSQENCEMLTALLDNIYGNWLDKVSSSKGKKREEIEAFINEGVYEVQRLKEEGWI 354
Query: 350 TNIQYDDEIIEMLKERLGQEKDKKIRMVDYKKYSRVRNWTLGLTGGKDQIAVIRASGSIS 409
T+I+YDDE+I MLKERLG +K+KK+ MVDY+KYSRVRNWTLGL+GGKDQIAVIRASGSI
Sbjct: 355 TDIRYDDEVISMLKERLGVKKEKKLSMVDYRKYSRVRNWTLGLSGGKDQIAVIRASGSII 414
Query: 410 RVRSPLS--SSGVVSEQFIEKIHRVKESKRYKAVVIRIDSPGGDALASDLMWREIKLLAA 467
RVRSPLS SSG+++EQFIEK+ V+ESKRYKAV+IRIDSPGGDALASDLMWREI+LLAA
Sbjct: 415 RVRSPLSLPSSGIIAEQFIEKVRSVRESKRYKAVIIRIDSPGGDALASDLMWREIRLLAA 474
Query: 468 SKPVIASMADVAASGGYYMAMAAGCIVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEII 527
SKPVIASM+DVAASGGYYMAMA G IVAENLTLTGSIGVVTGKFNLG LYEKIGFNKEII
Sbjct: 475 SKPVIASMSDVAASGGYYMAMATGTIVAENLTLTGSIGVVTGKFNLGNLYEKIGFNKEII 534
Query: 528 SRGKYAELTAAEQRSFRPDEAELFAKSAQNAYKLFRDKAALSRSMSADKMEEFAQGRVWT 587
SRGKYAEL AAEQR FRPDEA+LFAKSAQNAYK FRDKAA SRSM D+ME AQGRVWT
Sbjct: 535 SRGKYAELNAAEQRPFRPDEAKLFAKSAQNAYKQFRDKAAFSRSMDVDQMENVAQGRVWT 594
Query: 588 GNDAASRGLVDVLGGFSRAVAIAKQKAGIPQDKQVTLVEISRPSTSLPEILSGVGASLLG 647
GNDAASRGLVD +GG SRAVAIAKQKA IPQD+QV+LVE+SRPS +LPEILSG+G S++G
Sbjct: 595 GNDAASRGLVDAIGGLSRAVAIAKQKANIPQDRQVSLVELSRPSPTLPEILSGIGNSIIG 654
Query: 648 LNGTVKELLRDLTPSGGIQARMDGIMFERFEGASYANPIFNLIKDYISSI 697
++ TVKELL+DLT S G+QARMDGIMFER EG S NPIF L+KDY+SS+
Sbjct: 655 VDRTVKELLQDLTFSDGVQARMDGIMFERLEGTSNTNPIFTLMKDYLSSL 704
>OAY59330.1 hypothetical protein MANES_01G024300 [Manihot esculenta]
Length = 690
Score = 1036 bits (2679), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 510/706 (72%), Positives = 587/706 (83%), Gaps = 25/706 (3%)
Query: 1 MSKLLCTSHLNVVNICCSSSSSILSRS-------PLPIQLSKTLSLSDSQSSSLTKRRNL 53
MSKLL + + + +S+LS+S PLP+ + L + S +L
Sbjct: 1 MSKLLLIHSPHFTPVYRRTITSVLSKSHLCLSSTPLPLHSTSCLLHPLTHPSLFRPFPSL 60
Query: 54 HLSFSNSNPTRYLQIRAFESSSSSETTNQNLPNKEENEAENEVRASDENGSLKVNNNKDE 113
LS R L +RAF+SSS ++T Q E +E + ENGS+K N++D+
Sbjct: 61 ILS-------RNLSVRAFDSSSDTKTQKQE-------ETSDEKVSQSENGSIK--NSQDD 104
Query: 114 YPSGEFEFKEFNLWKSFVVKFRMLFAYPWERVRKGSVLSMKLRGEISDQLKSRFSSGLSL 173
YP+G+FEFKE W F+VK RML A+PWERVRKGSVL+MKLRG+ISDQLKSRFSSGLSL
Sbjct: 105 YPTGDFEFKEIGAWNRFLVKLRMLIAFPWERVRKGSVLTMKLRGQISDQLKSRFSSGLSL 164
Query: 174 PQICENFIKAAYDPRISGIYLQIEPLNCGWGKVEEIRRHILDFRKSGKFVVAYVPICQEK 233
PQICENFIKAAYDPRISGIYL IEPLNCGWGKVEEI+RHIL+F++SGKF+V Y+P C+EK
Sbjct: 165 PQICENFIKAAYDPRISGIYLHIEPLNCGWGKVEEIQRHILNFKQSGKFIVCYIPACREK 224
Query: 234 EYYIASACGELYCPPSAYFALYGLTVQSSFLGGIFEKVGIEPQVERIGKYKSAGDQLARK 293
EYY+A AC E+Y PPSAYF+LYGLTV++SFLGG+ EKVGIEP+V+RIGKYKSAGDQL RK
Sbjct: 225 EYYLACACDEIYVPPSAYFSLYGLTVEASFLGGVLEKVGIEPEVQRIGKYKSAGDQLTRK 284
Query: 294 SMSEENCEMLTALLDNIYGNWLDKISSTLGKKREEIENFVNEGVFQVERLKEDGWITNIQ 353
SMSE NCEMLT LLDNIYGNWLDKISST GKKREE+ENF+NEGV++VERLKE+G ITNI
Sbjct: 285 SMSEANCEMLTTLLDNIYGNWLDKISSTKGKKREELENFINEGVYEVERLKEEGLITNIH 344
Query: 354 YDDEIIEMLKERLGQEKDKKIRMVDYKKYSRVRNWTLGLTGGKDQIAVIRASGSISRVRS 413
YDDE+I MLKE+LG EKDK + +VDY KYSRVRNWTLGLTGG DQIAVIRASGSISRVRS
Sbjct: 345 YDDEVISMLKEKLGIEKDKILPVVDYGKYSRVRNWTLGLTGGADQIAVIRASGSISRVRS 404
Query: 414 PLS--SSGVVSEQFIEKIHRVKESKRYKAVVIRIDSPGGDALASDLMWREIKLLAASKPV 471
PLS SSG++ EQFIEKI +V+ESKRYKAVVIRIDSPGGDALASDLMWREI+LLA KPV
Sbjct: 405 PLSLPSSGIIGEQFIEKIRQVRESKRYKAVVIRIDSPGGDALASDLMWREIRLLAEKKPV 464
Query: 472 IASMADVAASGGYYMAMAAGCIVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEIISRGK 531
+ASM+DVAASGGYYMAMA G IVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEIISRGK
Sbjct: 465 VASMSDVAASGGYYMAMATGTIVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEIISRGK 524
Query: 532 YAELTAAEQRSFRPDEAELFAKSAQNAYKLFRDKAALSRSMSADKMEEFAQGRVWTGNDA 591
YAEL AAEQR RPDEAELFA+SAQNAYK FRDKAA SRSM+ DKMEE AQGRVWTG DA
Sbjct: 525 YAELLAAEQRPLRPDEAELFARSAQNAYKQFRDKAAFSRSMAVDKMEEVAQGRVWTGKDA 584
Query: 592 ASRGLVDVLGGFSRAVAIAKQKAGIPQDKQVTLVEISRPSTSLPEILSGVGASLLGLNGT 651
ASRGLVD GG +RA+AIAKQKA IPQDKQVTLVE+SRPS +LPEILSG+G+S+ G++ T
Sbjct: 585 ASRGLVDATGGLTRAIAIAKQKANIPQDKQVTLVELSRPSPTLPEILSGIGSSIAGVDRT 644
Query: 652 VKELLRDLTPSGGIQARMDGIMFERFEGASYANPIFNLIKDYISSI 697
+KELL+DL+ S G+QARMDGIMF+ EG+SYANPI +LIKD +SS+
Sbjct: 645 LKELLQDLSFSDGVQARMDGIMFQSLEGSSYANPILSLIKDILSSL 690
>XP_002268894.1 PREDICTED: serine protease SPPA, chloroplastic [Vitis vinifera]
Length = 686
Score = 1036 bits (2679), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 513/699 (73%), Positives = 592/699 (84%), Gaps = 15/699 (2%)
Query: 1 MSKLLCTSHLNVVNICCSSSSSILSRSPLPIQLSKTLSLSDSQSSSLTKRRNLHLSFSNS 60
MSKLL HL +I S ++ILS+ P++ + +S Q S +K +N FS S
Sbjct: 1 MSKLLHGCHLT--SIHRRSCAAILSK---PLRSIRVVSSLQPQFSP-SKPQNALSYFSPS 54
Query: 61 NPTRYLQIRAFESSSSSETTNQNLPNKEENEAENEVRASDENGSLKVNNNKDEYPSGEFE 120
R L +RAF+SSS T ++ ++E E + + D++G+L + +EYP+G+FE
Sbjct: 55 LHRRNLSLRAFDSSSE---TKSDVVSEEAGEKDYK----DDDGALSSTSLAEEYPTGDFE 107
Query: 121 FKEFNLWKSFVVKFRMLFAYPWERVRKGSVLSMKLRGEISDQLKSRFSSGLSLPQICENF 180
FKE + W SFVVK RML A+PWERVRKGSV +MKLRG+ISDQLKSRFSSGLSLPQICENF
Sbjct: 108 FKEMSGWMSFVVKLRMLIAFPWERVRKGSVFTMKLRGQISDQLKSRFSSGLSLPQICENF 167
Query: 181 IKAAYDPRISGIYLQIEPLNCGWGKVEEIRRHILDFRKSGKFVVAYVPICQEKEYYIASA 240
IKAAYDPRISGIYL IEPL+CGWGKVEEIRRHILDF+KSGKF+VAY P C EKEYY+ SA
Sbjct: 168 IKAAYDPRISGIYLHIEPLSCGWGKVEEIRRHILDFKKSGKFIVAYAPACGEKEYYLGSA 227
Query: 241 CGELYCPPSAYFALYGLTVQSSFLGGIFEKVGIEPQVERIGKYKSAGDQLARKSMSEENC 300
C ELY PPSAYF+LYGLTVQ+SFLGG+FEKVGIEPQV+RIGKYKSAGDQL RK+MSEENC
Sbjct: 228 CDELYAPPSAYFSLYGLTVQASFLGGVFEKVGIEPQVQRIGKYKSAGDQLTRKTMSEENC 287
Query: 301 EMLTALLDNIYGNWLDKISSTLGKKREEIENFVNEGVFQVERLKEDGWITNIQYDDEIIE 360
EMLTALLDNIYGNWLDKISS GKKRE+ ENF+NEGV+QVE+LKE+GWITNI YDDE+I
Sbjct: 288 EMLTALLDNIYGNWLDKISSAKGKKREDTENFINEGVYQVEKLKEEGWITNINYDDEVIS 347
Query: 361 MLKERLGQEKDKKIRMVDYKKYSRVRNWTLGLTGGKDQIAVIRASGSISRVRSPLS--SS 418
+LKERLGQ KDK + MVDY+KYS+VR WTLGL+GGKDQIAVIRASGSISRVRSP S S
Sbjct: 348 ILKERLGQPKDKNLPMVDYRKYSKVRKWTLGLSGGKDQIAVIRASGSISRVRSPFSIPGS 407
Query: 419 GVVSEQFIEKIHRVKESKRYKAVVIRIDSPGGDALASDLMWREIKLLAASKPVIASMADV 478
G+ SEQFIEKI V++SKRYKAV+IRIDSPGGDALASDLMWREI+LLAASKPVIASM+DV
Sbjct: 408 GITSEQFIEKIRSVRDSKRYKAVIIRIDSPGGDALASDLMWREIRLLAASKPVIASMSDV 467
Query: 479 AASGGYYMAMAAGCIVAENLTLTGSIGVVTGKFNLGKLYEKIGFNKEIISRGKYAELTAA 538
AASGGYYMAM AG IVAENLTLTGSIGVVTGKFNLG LYEKIGFNKEIISRG++AELTAA
Sbjct: 468 AASGGYYMAMGAGTIVAENLTLTGSIGVVTGKFNLGTLYEKIGFNKEIISRGRFAELTAA 527
Query: 539 EQRSFRPDEAELFAKSAQNAYKLFRDKAALSRSMSADKMEEFAQGRVWTGNDAASRGLVD 598
EQR FRPDEAELFAKSAQNAYK FRDKAA SRSM+ DKMEE AQGRVWTG DAASRGLVD
Sbjct: 528 EQRPFRPDEAELFAKSAQNAYKQFRDKAAFSRSMAVDKMEENAQGRVWTGKDAASRGLVD 587
Query: 599 VLGGFSRAVAIAKQKAGIPQDKQVTLVEISRPSTSLPEILSGVGASLLGLNGTVKELLRD 658
+GG SRAVAIAKQKA IPQD+ VTLVE+SRPS ++ EIL+G+G+S++G+ T+KELL+D
Sbjct: 588 AIGGLSRAVAIAKQKADIPQDRPVTLVELSRPSPTVSEILTGIGSSIVGVERTLKELLQD 647
Query: 659 LTPSGGIQARMDGIMFERFEGASYANPIFNLIKDYISSI 697
LT S G+QARMDGI+F++ E AS +NPIF L+KDY+SS+
Sbjct: 648 LTFSNGVQARMDGILFQKLEEASDSNPIFTLVKDYLSSL 686